5.2. Hemostasis and the role of thrombocytes. Blood coagulation. Fibrinolysis. Physiological anticoagulant mechanisms. Flashcards
I. Hemostasis
1. Definition of hemostasis
prevention of bleeding from a damaged vessel (prevention of hemorrhage)
I. Hemostasis
2. What are the stages of hemostasis?
PRIMARY HEMOSTASIS
1. Vasoconstriction
2. Increased tissue pressure
3. Formation of a platelet plug
SECONDARY HEMOSTASIS
Coagulation (formation of a blood clot)
I. Hemostasis
3A. What are the mechanism and role of vasoconstriction (primary hemostasis)?
1/ Mechanism:
- Upon injury of the blood vessel wall, there is an immediate response called vasoconstriction
- Vasoconstriction will decrease blood flow towards injured area, leading to decrease in blood loss
2/ Role: to give more time for more effective hemostatic processes to take place
I. Hemostasis
3B. What are the causes of vasoconstriction (primary hemostasis)?
1/ Neurogenic reflex
2/ Endothelin release
3/ Release of vasoconstrictor substances
I. Hemostasis
3B1. How does neurogenic reflex participate in vasoconstriction (primary hemostasis)?
produces vasoconstriction
I. Hemostasis
3B2. How does Endothelin release participate in vasoconstriction (primary hemostasis)?
- Endothelin Binds to to ET-receptor on SMC (smooth muscle cell), leading to vasoconstriction
I. Hemostasis
3B3. What are the vasoconstrictor substances that are released by thrombocytes? Consequences?
Chemical released by thrombocytes
- Thromboxane A2 (TXA2, synthesized by platelets)
=> Ca2+ signal
=> Vasoconstriction - Serotonin (synthesized by platelets)
- Epinephrine (not synthesized, taken up by platelets) => ⍺1 adrenergic receptor acting on smooth muscle contraction
I. Hemostasis
3C. Mechanism and role of Increased tissue pressure?
Increased tissue pressure contributes to hemostasis because:
- It decreases radius, which causes significant decrease in blood flow; decrease r by a factor of 2 would diminish flow by a factor of 16 according to - Poiseuille law.
E.g, We do this naturally by pressing a finger against a small cut to stop the bleeding.
I. Hemostasis
3D. What is the role of Platelet plug formation (step 3 in primary hemostasis)?
platelets plug small ruptures in the endothelium
I. Hemostasis
3E. What are the stages of Platelet plug formation (step 3 in primary hemostasis)?
1/ Adhesion
2/ Activation
3/ Aggregation
I. Hemostasis
3F. What are platelets?
platelets are fragments of megakaryocytes
I. Hemostasis
3G. What are characteristics (structure) with corresponding functions of platelets
1/ Have a dense tubular system
-> Acts as a Ca2+ storage
2/ Have dense granules containing ADP, serotonin and polyphosphates to activate platelets
3/ Have alpha granules which contain clotting factors
4/ Have actin + myosin for later contraction to reduce the size of the clot
5/ Have adhesion glycoproteins found on their surface
ex: GPIa/IIa, GPVI
I. Hemostasis - Stages of platelet formation
3H1. Why is platelet adhesion necessary? When will platelet adhesion occur?
1/ Platelets do not adhere to endothelium directly because platelets have negative surface charge and so do the endothelium (proteoglycans – heparan sulfate).
2/ Platelet adhesion occurs in response to ↑ shear stress at platelet surface or endothelial cells, and in response to vessel injury or humoral signals.
I. Hemostasis - Stages of platelet formation
3H2. What are the steps af platelet adhesion?
1/ Platelets first adhere to the injured space via glycoprotein-collagen binding with glycoprotein GP VI and GP Ia – IIa
=> This is a Direct platelet interaction
2/ Endothelial cells also release the von Willebrand factor (vWF) which also binds to exposed collagen and then create an indirect interaction between GP Ib – IX – B
I. Hemostasis - Stages of platelet formation
3I1. How are platelets activated?
Binding of GP VI to collagen initiates tyrosine phosphorylation cascade -> Phospholipase C (PLC) activation -> IP3 -> Ca2+-release = platelet is activated
*Note: Inositol trisphosphate or inositol 1,4,5-trisphosphate abbreviated InsP3 or Ins3P or IP3
I. Hemostasis - Stages of platelet formation
3I2. What are the 5 consequences of increased concentration of intracellular Ca2+?
- Exocytosis of granules
→ ADP, 5-HT, epinephrine, Ca2+, procoagulant factors, fibrinogen…
→ Positive feedback - Phospholipase A2 (PL2) activation
→ TXA2-production → positive feedback → platelet activation increased - Cytoskeletal rearrangement
→ platelets undergo shape change, increased surface - Exposure of GPIIb-IIIa integrin complex
→ fibrinogen binding, platelet aggregation - Phospholipid externalization
→ coagulation cascade
I. Hemostasis - Stages of platelet formation
3I3. The 5 consequences of increased contraction of intracellular Ca2+
-> What are the consequences of Exocytosis of granules?
→ ADP, 5-HT receptors (serotonin receptors), epinephrine, Ca2+, procoagulant factors, fibrinogen…
→ Positive feedback
I. Hemostasis - Stages of platelet formation
3I4. The 5 consequences of increased contraction of intracellular Ca2+
-> What are the consequences of PLA2 activation?
→ TXA2-production → positive feedback → platelet activation increased
I. Hemostasis - Stages of platelet formation
3I5. The 5 consequences of increased contraction of intracellular Ca2+
-> What are the consequences of 3. Cytoskeletal rearrangement?
→ platelets undergo shape change, increased surface