1.6. The development of the action potential in excitable cells: similarities and differences between distinct cells. Conduction of the action potential. Flashcards
I. Basics
1A. Definition of graded (electrotonic) potential?
A localized change in the membrane potential in response to a stimulus, which its size and duration proportional to the stimulus
- The distance over which the change in the potential decreases to a factor of 1/e of its maximal value is called the length or space constant
I. Basics
1C. What are the definitions of depolarization, hyper polarization and repolarization?
1/ Depolarization -> bring the membrane potential closer to firing (more positive)
2/ Hyperpolarization -> brings the membrane potential less likely to depolarize (more negative)
3/ Repolarization -> restores the difference in charge between the inside and outside of the cell membrane following depolarization
I. Basics
2A. What is the definition of AP?
a rapid, transient, self-propagating electrical excitation in the plasma membrane of an excitable cell (ex: nerve or muscle cells)
I. Basics
2B. What are the characteristics of AP?
It is characterized by a rapid depolarization followed by repolarization to the resting membrane potential
I. Basics
3. What the 3 ways that AP differ from other responses?
1) AP has a much larger amplitude
2) AP is generally propagated down the entire length of the axon without decrement (dying out)
3) AP is an ‘’all-or-none’’ response (becomes either a full-sized AP or fails to become one)
I. Basics
4. Make a comparison between AP and graded potential
I. Basics
5. What is the length/space constant?
Space or length constant is the distance over which the change in the potential decreases to a factor of 1/e of its maximal value
II. Development of action potential
1. What is the General mechanism of AP development?
Generally, action potentials develop as the result of a graded (electrotonic) potential which reaches the threshold voltage and stimulates a coordinated sequence of opening and closing of voltage-gated ion channels
=> resulting in depolarization and repolarization
II. Development of action potential
2. What are the characteristics of voltage-dependent Na+ channel? (threshold voltage, activation and inactivation)
1/ Ethr = -50 mV
2/ Rapid activation
3/ Inactivation
II. Development of action potential
3. What are the characteristics of voltage-dependent K+ channel? (threshold voltage, activation and inactivation)
Voltage-dependent K+-channel (delayed = low activation)
1/ Ethr = -50 mV
2/ Slow activation
3/ No inactivation
II. Development of action potential
4A. How does AP develop in neurons? (7 steps)
1) At rest,K+- permeability is high and Na+-permeability is low, leading to a membrane potential of -70mV
2) Influx of positive charges causes a depolarization to reach the threshold potential of -50mV, and trigger an action potential
3) When the AP is generated, Na+-ions rush into the neuron as the voltage-gated Na+ channels open, while the voltage-gated K+ channels remain closed
During the depolarization phase, the neuron cannot transmit another impulse whatsoever = absolute refractory period (voltage-gated Na+ channels are inactivated)
4) When the Na+-ions almost stop rushing into the neuron, the voltage-gated Na+ channels become inactivated
5) At the top of the spike (+35mV to +40mV), repolarization occurs (restoring the negative charge inside the neuron) due to 2 mechanisms:
- Slow inactivation gates close on the same VG Na+-channels with a delay
- Opening of the VG K+-channels
6) The VG K+ channels remain opened
-> Causes excessive flow of K+-ions out of the neuron = causes only negative potential inside (hyperpolarization / afterhyperpolarization / undershoot) to -87mV
7) It can fire a 2nd action potential, but a stronger stimulus than normal is required = relative refractive period
- If there is no stimulus, the VG K+-channels close and membrane potential returns to resting value
II. Development of action potential
- AP development in neurons (7 steps)
4B. In step 5, At the top of the spike (+35mV to +40mV), repolarization occurs (restoring the negative charge inside the neuron) due to 2 mechanisms
=> What are these 2 mechanisms?
1/ Slow inactivation gates close on the same VG Na+-channels with a delay
2/ Opening of the VG K+-channels allow inward movement of K+
II. Development of action potential
- AP development in neurons (7 steps)
4C. In step 6, The VG K+ channels remain opened
=> What are the consequences?
Causes excessive flow of K+-ions out of the neuron = causes only negative potential inside (hyperpolarization / afterhyperpolarization / undershoot) to -87mV
II. Development of action potential
5. What is the definition of Absolute refractory period?
Due to closure of the inactivation gates of the voltage-gated Na+-channels, no new AP can form no matter how strong the stimulus
II. Development of action potential
6. What is the definition of Relative refractory period?
Due to hyperpolarization via high K+-permeability through the slow K+-channels responsible for repolarization, any new AP formation would require a stronger than normal stimulus to reach the threshold
III. Ionic basis of AP
1. What is the ion basis of AP?
I = current, g = conductance
Eion calculated by Nernst equation
I ion = g ion * (Em - Eion)