49) Biotechnology in animal production: embryo manipulations (OPU, IVM, IVF, IVC) Flashcards

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1
Q

OPU

A
  • Ovum pick up
  • Collection of ova by sonographic control
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2
Q

IVM

A
  • In vitro maturation
  • Special media are required for this
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3
Q

IVF

A
  • In vitro fertilisation
  • Swim up method
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4
Q

Advantages of embryo manipulations

A
  • Successful in many species
  • Shortened generation interval
  • Gene transfer possible in early embryonic phase
  • Increased calf production per year
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5
Q

How often can OPU occur?

A

Can be as often as twice a week

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6
Q

Oocyte deep-freezing

A
  • Slow freezing
  • Low success rate
  • High embryo mortality rate
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7
Q

Embryo deep-freezing

A
  • Slow freezing
  • Fast freezing
    • Rapid cooling
    • 60% survival rate
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8
Q

IVC

A

In vitro culture

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