46) Preservation methods of genetic resources in breeding: in vitro

Question 1

In vitro

Answer 1

In glass

Question 2

Ex situ in vitro conservation

Answer 2

• Conservation external to the living animal
• In an artificial environment
• Under cryogenic conditions

Question 3

Give examples of in vitro preservation

Answer 3

• Cryoconservation of:
  
  • Embryos
  • Semen
  • Oocytes
  • Somatic cells

Question 4

In vitro preservation can last for…

Answer 4

Decades to Centuries

Question 5

Cryopreservation of semen

Answer 5

• Freezing
• Sufficient amount must be stored to produce the no. of backcross generations required

Question 6

Cryopreservation of oocytes

Answer 6

• Embryos can be produced in vitro from mature oocytes
• Frozen for prolonged periods prior to IVF to produce embryos

Question 7

Cryopreservation of embryos

Answer 7

• Frozen, thawed, transferred into females
• Successful in Ruminants
• Experimental in Equine
• Swine require the death of the female
• Allow full recovery of the genome