48) Biotechnology in animal production: semen deep freezing, sperm-sexing Flashcards
1
Q
How is semen frozen?
A
- Semen placed into ionic solution
- Cryoprotectants added to the solution
- Sample frozen in liquid nitrogen (196°C)
2
Q
Give a commonly used cryoprotectant
A
Glycerol
3
Q
Individual semen doses are frozen in…
A
Straws
Guarantees optimal sanitary condition and permanent ID of each dose
4
Q
Cryopreservation is common in…
A
- Bull
- Stallion
- Buck
- Ram
- Boar
- Dog
5
Q
Advantage of freezing
A
- Export-import
- International commercialisation of semen
- Gene preservation
- Establishment of gene banks
6
Q
Sperm sexing
A
Choosing:
- Healthier sperm
- Sexes of offspring
7
Q
Sperm sexing methods
A
- Sedimentation
- Centrifugation
- Fluoroscopic flow cytometry
- Lifespan + speed of spermatazoa
- SRY-gene
- Y-specific DNA
- DNA content
8
Q
The advantage of sperm sexing
A
Dramatic effect on the efficiency of:
- Milk production
- Meat production
9
Q
Female sperm have …% more DNA than males
A
3-4%
10
Q
Sperm cell DNA uptake of the fluorescent dye
A
Female-determining sperm give off more light as they have more DNA
11
Q
Sperm sexing has …% accuracy
A
90%
12
Q
Why isn’t sperm sexing widely used?
A
Expensive
