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Microbiology > 4.1: Microbial Growth and Control > Flashcards

4.1: Microbial Growth and Control Flashcards

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1
Q

______ is a liquid or solid formulated to support microbial growth.

A

Growth media

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2
Q

What is a Growth media?

A

Growth media is a liquid or solid formulated to support microbial growth.

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3
Q

The Growth media contains essential ___1___ to provide the microbe with a source of ___2___, which, in turn, encourages its ___3___.

A
  1. Nutrients
  2. Carbon/Energy
  3. Expansion
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4
Q

The Growth media contains essential……

A

……nutrients to provide the microbe with a source of carbon/energy, which, in turn, encourages its expansion.

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5
Q

The nutrient composition of Growth Media’s often includes a rich source of ___1___, ___2___, and ___3___.

A
  1. Sugars
  2. Amino Acids
  3. Vitamins
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6
Q

Such media can also be referred to as a ___1___ and is commonly used to grow microbes in a ___2___.

A
  1. Nutrient Broth
  2. Suspension
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7
Q

What is the most common nutrient broth in the lab?

A

LB media (lysogeny broth)

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8
Q

“LB” media stand for?

A

Lysogeny Broth

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9
Q

What is LB media (lysogeny broth)?

A

LB media (lysogeny broth) is a nutritionally rich liquid known for its ability to grow a vast array of microbes.

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10
Q

______ is a nutritionally rich liquid known for its ability to grow a vast array of microbes.

A

LB media (lysogeny broth)

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11
Q

A researcher wanting to establish conditions where various types of bacteria will grow, while others will not would require a specialized media called what?

A

Selective

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12
Q

What does being Selective mean in regards to Growth Media?

A

When a researcher wants to establish conditions where various types of bacteria will grow, while others will not.

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13
Q

A researcher wanting to establish conditions where several types of microbes can be grown simultaneously while being able to distinguish one type from another would require a specialized media called what?

A

Differential

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14
Q

What does being Differential mean in regards to Growth Media?

A

A researcher wanting to establish conditions where several types of microbes can be grown simultaneously while being able to distinguish one type from another.

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15
Q

The two general classifications of specialized media are what?

A

Selective
Differential

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16
Q

______ allows for only the growth of certain microbes and, by extension, restricts the growth of all others.

A

Selective media

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17
Q

Selective media allows for……

A

……only the growth of certain microbes and, by extension, restricts the growth of all others.

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18
Q

Using the Selective Media approach can be accomplished in a variety of ways including……….

A

…….using limiting amounts of nutrients, varying degrees of pH (being either very acidic or very basic media), or various chemical additives that limit unwanted microbial growth (i.e. antibiotics).

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19
Q

______ is often used in medical laboratories for the cultivation of human pathogens such as Neisseria meningitides, the potential causative agent of meningitis.

A

Selective Media

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20
Q

Selective media is often used in medical laboratories for the cultivation of human pathogens such as ______, the potential causative agent of meningitis.

A

Neisseria meningitides

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21
Q

What is Neisseria meningitides?

A

the potential causative agent of meningitis

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22
Q

What is the potential causative agent of meningitis?

A

Neisseria meningitides

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23
Q

______ is a relatively slow-growing organism, other bacteria, mold, or fungi often outgrow the sample

A

Neisseria meningitides is a relatively slow-growing organism, other bacteria, mold, or fungi often outgrow the sample

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24
Q

Neisseria meningitides is a relatively……

A

……slow-growing organism, other bacteria, mold, or fungi often outgrow the sample

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25
Q

______ distinguishes between two, often related, microbes.

A

Differential media

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26
Q

The two microbes ___1___ and ___2___ are both Gram-negative but can be distinguished by the presence (or absence) of lactose fermentation.

A
  1. Escherichia coli
  2. Salmonella
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27
Q

The two microbes Escherichia coli and Salmonella are both ___1___ but can be distinguished by the presence (or absence) of ___2___.

A
  1. Gram-negative
  2. lactose fermentation
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28
Q

If grown on the same differential media, ___1___ ferments lactose and turns the culture red. In contrast, ___2___ does not ferment lactose, and the culture remains white/tan in color.

A
  1. E. coli
  2. Salmonella
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29
Q

______ is used to grow fastidious microorganisms—organisms with complex growth requirements such that if absent will not grow.

A

Enriched media

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30
Q

Enriched media is used to grow…….

A

……fastidious microorganisms—organisms with complex growth requirements such that if absent will not grow.

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31
Q

What are fastidious microorganisms?

A

Organisms with complex growth requirements such that if absent will not grow.

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32
Q

What is are organisms with complex growth requirements such that if absent will not grow.

A

fastidious microorganisms

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33
Q

True or False: Medias can only ever be a single instance of a selective, differential, and enriched media. These approaches cannot be combined.

A

False. Medias can be a combination of selective, differential, and enriched.

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34
Q

Growth refers to what?

A

The number of organisms in population

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35
Q

The number of organisms in population

A

Growth

36
Q

Culture refers to what?

A

The process of growing microbes in a laboratory or collection of isolated microbes.

37
Q

The process of growing microbes in a laboratory or collection of isolated microbes..

A

Culture

38
Q
A
39
Q
A
40
Q

What are Trace Elements?

A

Minuscule amount necessary to support metabolism

41
Q

Minuscule amount necessary to support metabolism

A

Trace Elements

42
Q

What are Growth Factors?

A

Organic Chemical needed in small quantities to support metabolism

43
Q

Organic Chemical needed in small quantities to support metabolism

A

Growth Factors

44
Q
A
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46
Q
A
47
Q
A
48
Q
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49
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A
50
Q

Extreme Thermophiles survive at what Temperatures(Celsius)?

A

65-120

51
Q

Thermophiles survive at what Temperatures(Celsius)?

A

40-75

52
Q

Mesophiles survive at what Temperatures(Celsius)?

A

10-50

53
Q

Psychrotrophs survive at what Temperatures(Celsius)?

A

0-30

54
Q

Psychrophiles survive at what Temperatures(Celsius)?

A

-20-10

55
Q

Human Body temperature is what Temperature(Celsius)?

A

37

56
Q

Room Temperature(Celsius) is what?

A

20

57
Q

Water freezes at what temperature(Celsius)?

A

0

58
Q

Water boils at what temperature(Celsius)?

A

100

59
Q

What is note worthy about Extreme Thermophiles in regards to a lab setting?

A

They can withstand Autoclaving

60
Q

What organisms survive at Temperatures(Celsius) of 65-120?

A

Extreme Thermophiles

61
Q

What organisms survive at Temperatures(Celsius) of 40-75?

A

Thermophiles

62
Q

What organisms survive at Temperatures(Celsius) of 10-50?

A

Mesophiles

63
Q

What organisms survive at Temperatures(Celsius) of 0-30?

A

Psychrotrophs

64
Q

What organisms survive at Temperatures(Celsius) of -20-10?

A

Psychrophiles

65
Q

______ confirms an infection diagnosis by isolating and growing the microorganism for identification purposes

A

Culturing

66
Q

Culturing does what?

A

Confirms an infection diagnosis by isolating and growing the microorganism for identification purposes

67
Q

______ is a sample of the microbe

A

Inoculum

68
Q

What is an Inoculum?

A

a sample of the microbe

69
Q

______ can be Broth(liquid) or Solid media

A

Medium

70
Q

The Medium can be ___1___ or ___2___ media.

A
  1. Broth(liquid)
  2. Solid media
71
Q

______ are a cluster of cells arising from one parent cell

A

Colonies

72
Q

Colonies are a………

A

cluster of cells arising from one parent cell

73
Q

____ is the time the microbes are being cultured

A

Incubation Period

74
Q

What is Incubation Period?

A

Is the time the microbes are being cultured

75
Q

True or False: You can see colonies start growing on solid mediums.

A

True

76
Q

What is a DEFINED Growth Media?

A

Known (exact) quantities of all ingredients

77
Q

What is an UNDEFINED Growth Media?

A

Contains mixtures of unknown ration (yeast or beef extract)

78
Q

Adding blood to a Differential Media turns it into a ______.

A

Blood Agar

79
Q

What type of blood is usually used in a Blood Agar?

A

Sheep Blood

80
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A
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85
Q
A

Microbiology (39 decks)

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