2.13 Transcription Flashcards
Process of synthesizing RNA from a DNA template
Transcription
Transcription is the first step of ___
Gene expression
Runs from 3’ -> 5’ direction
Template/antisense/non-coding strand
Synthesized RNA appears similar to the ____ strand, which runs from 5’-> 3’ direction
Non-template/sense/coidng strand
Corresponds to the first nucleotide expressend in the 5’ end of the synthesized RNA
+1
Serves as the apoenzyme
Protein component of the RNA polymerase
Core enzyme
Core enzyme is composed of 5 units: __
alpha-alpha-beta-beta’-omega
Assembly of subunits
alpha (1)
Promoter-binding site
Recognizes the promoter region
alpha (2)
Nucleotide binding site
beta
Catalytic site
Possesses inherent DNA-DNA helicase activity in the breakage of hydrogen bonds
Also binds incoming NTPs to the growing mRNA strand via phosphodiester bonds
beta’
Stabilizes RNA molecule
omega
The only responsible for RNA elongation
5’->3’ RNA polymerase
Non-protein component that activates the core enzyme
Coenzymes
Increase RNA polymerase affinity for promoter region
Unique for each RNA synthesized
Sigma factor
Signal end of transcription only for rho-dependent termination
Termination factors
Termination factors are also called the ___
rho factor
3 processes of prokaryotic transcription
Initiation
Elongation
Termination
Occurs at the promoter region
Initiation
Usually located -8 to -10 bases to the left of +1 (downstream)
Pribnow Box
Base sequence of Pribnow box
5’-TATAAT-3’
Usually located -35 bases to the left of +1 (downstream)
-35 sequence
Base sequence of -35 sequence
5’-TTGACA-3’
Sigma factor is released
Takes place through the RNA polymerase
Elongation
Recognizes the C-rich regions near the 3’ end
rho-dependent termination
rho factors has the ____, to separate the template DNA stand and the synthesized RNA strand at the end of transcription
RNA-DNA helicase activity
RNA forms a hairpin turn via palindromic sequences
rho-independent temination
Bonds have high melting point and as strong and stable
G-C rich regions
Facilitates the separation of newly synthesized RNA strand
U-A rich regions
Binds to and changes the morphology of beta subunits of bacterial RNA polymerase
Blocks or suppresses the initiation step
Rifampin