1S [LEC]: Minor Enzymes Flashcards
Minor enzyme present in the serum, pancreas, liver, heart, and white matter of CNS
Pseudocholinesterase
Enzyme that hydrolyzes acetylcholine to choline and acetic acid
Cholinesterase
Enzyme important in the transmission of nerve impulse
Cholinesterase
T/F: An increased cholinesterase value is significant in liver disease, metastatic carcinoma, and insecticide poisoning
False (no clinical significant if increased, only if decreased)
Identify the method of cholinesterase determination:
Measures decrease in pH resulting from the liberation of acetic acid
Michel Method (electrometric method)
Identify the method of cholinesterase determination:
Measures the liberation of CO2 from the formation of acetic acid as acetylcholine is hydrolyzed
Manometric method
Identify the method of cholinesterase determination:
Uses butylcholine (thiolester) as substrate
Ellman technique (photometric enzyme reaction)
Minor enzyme responsible in the splitting of fructose 1,6-diphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate
Aldolase
Enzyme responsible in the hydrolysis of N-terminal residues from certain peptides and amides containing free amino groups
Leucine aminopeptidase (LAP)
Tissue sources of LAP
Urine, serum, blie
LAP method of determination using fluorometric and colorimetric method
Goldbarg and Rutenberg
OCT method of determination that uses isotopic and microdiffusion technique
Reichard and Reichard
Tissue source of OCT
Liver
T/F: No elevations of G6PD is seen in hepatic disorders
True
Ornithin carbamoyl transferase is responsible in the reversible conversion of ornithine to ___
Citrulline
The conversion of ornithin to citrulline, which is the primary responsibility of the OCT is also referred to as the synthesis of ___
Urea
Enzyme found in the erythrocytes, nerve tissues/cells, brain
True cholinesterase
Cholinesterase enzyme is normally synthesized in the ___
Liver
A decrease enzyme that decreases in insecticide/ organophosphorus poisoning
Cholinesterase
Elevated minor enzyme in skeletal disorder such as muscular dystrophy
Aldolase (ALS)
Minor enzymes elevated in hepatobiliary jaundice
OCT, LAP
