1S [LAB]: ACP

Question 1

A hydrolase that catalyzes hydrolysis of various phosphosmonoesters

Answer 1

Acid phosphatase

Question 2

Optimal pH level of acid phosphatase

Answer 2

5

Question 3

Acid phosphatase converts phosphomonoester and water to ___ and ___

Answer 3

alcohol
phosphate ion

Question 4

Richest source of ACP

Answer 4

Prostate

Question 5

Diseases associated with ACP

Answer 5

Paget’s disease
Gaucher’s disease

Question 6

ACP isoenzyme inhibited by tartrate

Answer 6

Prostatic ACP

Question 7

T/F: Non-prostatic ACP are not inhibited by tartrate

Answer 7

True

Question 8

T/F: Prostatic ACP is directly measured

Answer 8

False (Prostatic ACP is a derived measure)

Question 9

Identify the ACP method:

Orthophosphoric monoesters; same with that of ALP except for pH

Answer 9

Substrates

Question 10

Identify the ACP method:

B-glycerophosphate or Na-glycerophosphate

Answer 10

Bodansky method

Question 11

Identify the ACP method:

Phenylphosphate or disodium phenylphosphate

Answer 11

King-Armstrong or Gutman-Gutman method

Question 12

Identify the ACP method:

a-1-naphthyl phosphate as substrate

Answer 12

Babson and Reed method

Question 13

Identify the ACP method:

Substrate of choice for quantitative end point reactions

Answer 13

Thymolphthalein monophosphate