1S [LEC]: Enzymes (GGT, AMS, LPS)

Question 1

Enzyme involved in peptide and protein synthesis, regulation of tissue glutathione levels

Answer 1

GGT

Question 2

Enzyme markedly elevated in biliary tract obstruction

Answer 2

GGT

Question 3

Enzyme responsible in the breakdown of starch and glycogen with products glucose, maltose, and dextrin

Answer 3

Amylase

Question 4

Cofactors require in amylase

Answer 4

Ca2+, Cl-

Question 5

In acute pancreatitis, the rise of AMS is observed ___ hours after onset of attack and peak at ___

Answer 5

2-12 hrs
24 hrs

Question 6

In acute pancreatitis, the AMS will return normal within ___

Answer 6

3-5 days

Question 7

Effect of oxalates and citrates in AMS determination

Answer 7

False decrease

Question 8

Effects of morphine and opiates in AMS determination

Answer 8

False increase

Question 9

Enzyme that hydrolyses that ester linkages of fats to produce alcohols and fatty acids

Answer 9

Lipase

Question 10

LPS elevation in pancreatic disorder lasts within ___

Answer 10

5 days

Question 11

The more specific enzyme in pancreatic disorder

Answer 11

LPS

Question 12

T/F: In LPS determination, the extant of elevation is proportional to the severity of pancreatic condition

Answer 12

False

Question 13

Identify the LPS method:

Fast in solution create cloudy emulsion

Answer 13

Turbidimetric enzyme reaction

Question 14

Activators required in LPS determination

Answer 14

Albumin or ionized calcium

Question 15

Inhibitors of LPS determination

Answer 15

Heavy metals like quinine

Question 16

The indicator reaction in LPS quantification is monitored through a ___ in turbidity

Answer 16

decrease

Question 17

Identify the LPS method:

Measures liberated fatty acids released by alkaline titration after 24 hours incubation using phenolphthalein as indicator

Answer 17

Cherry Crandall method

Question 18

Identify the LPS method:

Same as Cherry Crandall but uses thymolphthalein as indicator

Answer 18

Sigma Tietx

Question 19

Indicator of Cherry Crandall method of LPS determination

Answer 19

Phenolphthalein

Question 20

Reference range of LPS

Answer 20

<38 U/L

Question 21

Patients taking warfarin, phenobarbital, and phenytoin will have ___ increase in GGT

Answer 21

4x

Question 22

Alcoholism will increase GGT by how many fold?

Answer 22

2-3x

Question 23

T/F: The Szasz method of GGT determination is not affected by hemolysis

Answer 23

True

Question 24

Smallest enzyme that can be filtered by the glomerulus and is excreted in the urine

Answer 24

Amylase

Question 25

The level of AMS in intraabdominal diseases

Answer 25

<500 Somogyi units/dL

Question 26

Unit of measurement for AMS enzyme activity

Answer 26

Somogyi units

Question 27

Level of AMS in acute pancreatitis

Answer 27

250-1000 Somogyi units/dL

Question 28

Effect of hyperlipidemia in AMS measurement

Answer 28

False decrease

Question 29

LPS isoenzyme that is the most clinically significant

Answer 29

L2

Question 30

Enzyme used to differentiate the cause of AMS elevation

Answer 30

LPS

Question 31

Substrate used in LPS determination

Answer 31

Olive oil or triolein

Question 32

The titration method of LPS determination

Answer 32

Cherry Crandall method

Question 33

Effect of hemolysis in LPS

Answer 33

False decrease

Question 34

Stability of LPS at RT

Answer 34

1 week

Question 35

Enzyme that prevents the oxidation of hemoglobin

Answer 35

G6PD

Question 36

Deficiency of this enzyme can cause hemolytic anemia

Answer 36

G6PD

Question 37

Enzyme involved in peptide and CHON synthesis, regulation of tissue glutathione levels, transport of AA across CM

Answer 37

GGT