1S [LEC]: Liver function pt. 2 Flashcards
In Muelengracht Method of determining icterus index, the serum is diluted with ___
NSS
In Muelengracht Method of determining icterus index, the diluted fluid is compared with ___
0.01% K2Cr2O7
In Newberger Method of determining icterus index, the serum is diluted with ___
Sodium citrate
Specimen of choice of the classic diazo reaction of bilirubin determination
First morning urine
In the Evelyn and Malloy method of bilirubin determination, which reagent acts as a buffer?
Sodium acetate
In the Evelyn and Malloy method of bilirubin determination, which reagent terminates the reaction by destroying the diazo reagent?
Ascorbic acid
Composition of Diazo rgt
Sulfanilic acid
HCl
Sodium nitrite
T/F: Jendrassik & Grof is more sensitive than Evelyn & Malloy
True
Accelerator of Evelyn & Malloy bilirubin determination method
50% Methanol
End color of Evelyn & Malloy bilirubin determination method
Pink to purple
Accelerator of Jendrassik & Grof bilirubin determination method
Caffeine Sodium Benzoate
End color of Jendrassik & Grof bilirubin determination method
Blue
One of the advantages of Jendrassik & Grof bilirubin determination method is that it is not affected by hemoglobin up to ___
750 mg/dL
Component of the Ehrlich’s reagent
p-dimethyl aminobenzaldehyde
Reagent in the Ehrlich’s method that stops the reaction and minimizes the combination of other chromogens
NaCH3COO
Product of Ehrlich’s method of urine urobilinogen determination
Red colored compound
Reducing agent in the Ehrlich’s method of urine urobilinogen that maintains urobilinogen in reduced state
Ascorbic acid
Specimen of choice in Ehrlich’s method of urine urobilinogen determination
2 hour-fresh urine
The bilirubin will decay by how many percent when exposed to light?
30-50%
Conversion factor for bilirubin determination
17.1
This reagent converts back urobilin to urobilinogen
Alkaline ferric hydroxide
Fecal urobilinogen is measured through what method/s?
Ehrlich’s aldehyde
Watsons’ Reaction
In the Hay test of bile salt determination, what reagent is sprinkled to 5mL of urine?
Sulfur powder
In the Hay test of bile salt determination, if the sulfur powder floats at the top, the result is ___
Negative
In the Hay test of bile salt determination, if the sulfur powder sinks at the bottom, the result is ___
Positive (obstructive jaundice)
Radioisotope used in the Rose Bengal Test of excretory function
Iodine 131
Methods used in testing for excretory function
Bromsulphalein test
Rose Bengal test
In the Quick’s test or Hippuric Acid test, the patient is given a ___
Sodium benzoate meal
