1S [LAB]: AMS Flashcards
A hydrolase enzyme that catalyzes the breakdown of starch and glycogen with products glucose, maltose, dextrin
Amylase
Smallest enzyme and can be filtered by glomerulus
Amylase
Ions required to activate amylase
Ca2+, Cl-
Major sources of amylase
Pancreas
Salivary gland
Minor sources of amylase
Skeletal muscle
Liver
Fallopian tube
A condition where the AMS combines with Ig to form a large complex that is not filterable by the glomerulus
Macroamylasemia
Identify the AMS isoenzyme:
Predominates in urine
P-type
Identify the AMS isoenzyme:
Markedly elevated in acute pancreatitis
P3
Identify the AMS isoenzyme:
Predominates in serum
S-type
T/F: P-type AMS migrates faster than S-type AMS
False (S-type migrates faster)
S-type AMS is inhibited by ___
Wheat germ lectin
Identify the amylase method:
Measures the disappearance of starch substrate
Amyloclastic
Identify the amylase method:
Measures the appearance of the product
Saccharogenic
Identify the amylase method:
Measures the increasing color from production of product coupled with chromogenic dye
Chromogenic
Identify the amylase method:
Coupling of several enzyme systems to monitor amylase activity
Continuous monitoring
Identify the amylase method:
Measures the time required to reach the chromic point
Amyloclastic/ Iodometric method
In amyloclastic or iodometric method of AMS, fast disappearance means ___ AMS activity
high
Identify the amylase method:
Starch substrate is hydrolyzed by AMS to its constituent carbohydrate molecule that has reducing property like sugars
Saccharogenic
In saccharogenic method of AMS, the amount of reducing sugar is reported in what unit?
Somogyi units
The number of mg of glucose in 30 mins at 37C at specific assay conditions
Somogyi units
Identify the amylase method:
Employs a starch substrate with chromogenic dye that forms an insoluble dye-substrate complex
Chromolytic/ chromogen labelled substrate method (colorimetric)
T/F: The endpoint of colorimetric method of AMS, smaller dye-substrate, is water soluble
True
In coupled enzyme reaction or continuous monitoring method, the change in absorbance of NAD+ is measured at what wavelength and pH?
340 nm
pH 6.9
Substrate used in coupled enzyme reaction method of AMS
Maltotetrase or maltopentoase
In the AMS determination, the increase in absorbance is measured at what wavelength and temperature?
405 nm
37C
Factor to compute amylase
9227
