1S [LAB]: ALP Flashcards
Optimal pH level for alkaline phosphataes
9-10
Clinical significance of alkaline phosphatase
Hepatobiliary
Bone disorders
In ALP isoenzyme differentiation, heat stability is tested at what temperature and how long?
56C for 10 mins
In ALP isoenzyme differentiation, if after heating the remaining activity is <20%, the source of ALP elevation is the ___
bone
In ALP isoenzyme differentiation, if after heating the remaining activity is >20%, the source of ALP elevation is the ___
Liver
Phenylalanine inhibits ___% of intestinal and ___% of placental ALP
75
80
Most heat stable ALP isoenzyme
Regan isoenzyme
Regan isoenzyme can resist denaturation up to what temperature and how long?
65C for 30 mins
T/F: Regan isoenzyme is both stable under heat and phenylalanine
False (it is inhibited by phenylalanine)
The variant of Regan isoenzyme that is inhibited by L-leucine
Nagao-variant of Regan
ALP isoenzyme seen in metastatic CA of pleural surfaces
Nagao-variant of Regan
ALP isoenzyme seen in adenocarcinoma of the pancreas and bile duct
Nagao-variant of Regan
T/F: An increase in ALP can be non-pathologic
True (in the case of healing bone fractures and pregnancy)
Identify the ALP method:
B-glycerophosphate or Na-glycerophosphate
Bodansky method
Identify the ALP method:
Phenylphosphate or disodium phenylphosphate
King-Armstrong or Gutman-Gutman Method
Identify the ALP method:
A continuous monitoring technique of ALP activity
Bowers and McComb
Identify the ALP method:
Based on the molar absorptivity of p-nitrophenol
Bowers and McComb
Diet may elevate ALP level in which group/s of secretors
B and O
ALP determination requires what ions as activators?
Mg2+ and Mn2+
Buffer used in ALP determination
Diethanolamine buffer (pH 7.5)
Activator used in ALP determination
Magnesium chloride
Substrate used in ALP determination
P-nitrophenyl phosphate
Factor to compute ALP
2750
