15. Serum Iron and Iron Binding Capacity Flashcards
What is Iron an Essential Component of?
Haemoglobin
Myoglobin
Certain Enzymes
What is Functional Iron?
2/3 or MORE of Body’s Total Iron
IN Normoblasts + Erythrocytes
What is Iron Involved in?
Enzymatic Reactions = Redox Reactions
What Transports Iron?
Plasma Transferrin
1 Molecule BINDS to 2x Atoms of Ferric Ion
What are the 2 Storage Irons, and
Where are they Located?
- Ferritin
- Hemosiderin
- In MFs of Reticuloendothelial System
1| What is Ferritin?
Water-Soluble Complex of:
- Ferric Salt
- Apoferritin (Protein)
2| What is Hemosiderin?
Water INSOLUBLE
Consists of Aggregates of Ferric Oxyhydroxide q/ Degraded Protein Shell
Which Specimen is Used for Iron Investigation?
Serum
Why is Plasma UNACCEPTABLE Iron Investigation?
Anticoagulant Interferes with Most Procedures
What is the Reference Range of Serum IRON in MALES?
11.6 – 31.7 µmol / L
What is the Reference Range of Serum IRON in FEMALES?
9 – 30.4 µmol / L
What is the Reference Range of Total Iron Binding Capacity, TIBC?
45 - 66 µmol / L
What is the Reference Range of Serum FERRITIN in MALES?
20 - 250 µg / L
What is the Reference Range of Serum FERRITIN in FEMALES?
15 - 150 µg / L
What is the Reference Range of Serum TRANSFERRITIN in MALES?
2 - 4 g / L
What are the Indications for Investigating Serum Iron?
Tiredness
Fatigue
Anaemia
What are the Analytical Methods for Serum Iron?
- Photometry
2. Ref = Atomic Absorption Spectrophotometry
What are the Analytical Methods for Total Iron Binding Capacity?
- Chemical Methods = Saturation with Transferrin w/ Iron
2. Immunochemical = Tf as Protein
What are the Analytical Methods for Ferritin?
- ELISA
2. Immunofluoresence
What are the Levels of Iron AND TIBC / Transferrin in IRON DEFICIENCY?
LOW Iron
HIGH TIBC / Transferrin
What are the Levels of % Transferrin Saturation AND Ferritin in IRON DEFICIENCY?
LOW % Transferrin Saturation
LOW Ferritin
What are the Levels of Iron AND TIBC / Transferrin in HEMOCHROMATOSIS?
HIGH Iron
LOW TIBC / Transferrin
What are the Levels off % Transferrin Saturation AND Ferritin in HEMOCHROMATOSIS?
HIGH % Transferrin Saturation
HIGH Ferritin
What are the Levels of Iron AND TIBC / Transferrin in CHRONIC ILLNESS?
LOW Iron
LOW TIBC / Transferrin
What are the Levels off % Transferrin Saturation AND Ferritin in CHRONIC ILLNESS?
LOW % Transferrin Saturation
Normal / HIGH Ferritin
What are the Levels of Iron AND TIBC / Transferrin in HEMOLYTIC ANEMIA?
HIGH Iron
Normal / LOW TIBC / Transferrin
What are the Levels off % Transferrin Saturation AND Ferritin in HEMOLYTIC ANEMIA?
HIGH % Transferrin Saturation
HIGH Ferritin
What are the Levels of Iron AND TIBC / Transferrin in SIDEROBLASTIC ANEMIA?
Normal / HIGH Iron
Normal / LOW TIBC / Transferrin
What are the Levels off % Transferrin Saturation AND Ferritin in SIDEROBLASTIC ANEMIA?
HIGH % Transferrin Saturation
HIGH Ferritin
What are the Levels of Iron AND TIBC / Transferrin in IRON POISONING?
HIGH Iron
Normal TIBC / Transferrin
What are the Levels off % Transferrin Saturation AND Ferritin in IRON POISONING?
HIGH % Transferrin Saturation
Normal Ferritin
