wk 10 lec 2 Flashcards

1
Q

volatile vs non volatile acids

A

volatile= can breath off as carbon dioxide
- via bicarbonate buffer (non volatile can also be buffered by bicarbonate to minimize large pH changes but cant breath off- still need to secrete protonated part via kidneys)

non volatile- cant breath off

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2
Q

examples of nonvolatile acids

A

lactic acid, ketone bodies, sulphuric acid, HcL from protein metabolism or consumptions

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3
Q

orange juice vs cranberry juice acids

A

cranberry- non volatile bc benzoic acid

orange juice is citric acid which turns into citrate into CO2 so its volatile

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4
Q

3 mechanisms of hydrogen ion regulation

A
  1. fluid chemical buffers (IC and EC)
    –> rapid: bicarbonate, phosphate, ammonia, proteins
  2. respiratory system
    –> rapid, Co2, NOT nonvolatile
  3. renal system
    –> slower, non-volatile, secrete H+, reabsorb and generate new HCO3-
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5
Q

phosphate, proteins, bicarbonate, ammonia

where are theses buffer systesm

A

phospahte: renal tubular

proteins: intracellular (i.e. hemoglobin)

bicarbonate: ECF

ammonia: renal tubular

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6
Q

protein intraceullar buffer

A

donate or accept amino acids (bind or release H+)

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7
Q

most important amino acids at physiological pH

A

histidine and cysteine

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8
Q

why is hemoglobin a good intraceullar buffer

A

38 histidine residues

carries CO2 through blood when O2 is low

move co2/hco3 across RBC membrane

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9
Q

phosphate buffer system for

A

intraceullar buffer

urinary buffer to stabilize pH

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10
Q

most important ECF buffer

A

bicarbonate

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11
Q

normal values for HCO3, CO2 and pH

A

HCO3= 24 (22-26)
CO2= 40 (35-45)
pH= 7.40 (7.35-7.45)

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12
Q

what to carry H+ out into urine

A

ammonia (regulated)

also a bit of phospahte

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13
Q

where is ammonia made

A

proximal tubule

then secreted into tubular urine

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14
Q

ammonium reaborbed

A

NH4+ reabsorbed in thick ascending limb

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15
Q

pH of tubular urine along tubule

what happens in collecting duct and via what

A

continues to decrease (get more acidic)

NH3 goes into acidic collecting duct and trapped as NH4+ and eliminate H+

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16
Q

how does 80% of HCO3 get reabsorbed in proximal tubule

A

not directly (bc not permeable to luminal membranes)

combines with H+ to make H2CO3 via carbonic anhydrase and then dissociate to CO2 and H2o

then secrete H+ via Na/H countertrasnprot

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17
Q

production of new HCO3 via what amino acid and what process

A

in PCT make new bicarbonate and ammonia

from glutamine metabolism

ammonia to carry H+

gluconeogenesis – PEP from
glutamine is converted to
glucose

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18
Q

how to excrete lots of acid at pH of 4.5 and not too acidici

A

buffer with phospahte and ammonium

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18
Q

how to make urine acidic

which ion
which part

A

in collecting duct

H+ ATPase

and H2CO3 dissociate to HCO3 and H+

19
Q

produce ammonium if ECF to acidic. where

20
Q

urinary buffer

A

ammonia in PCT (to balance H+)

21
Q

alpha and beta intercalated cells in collecting duct impacts

A

alpha (acidosis) secret H+, reabsorb HCO3- (and exchange K+ for H+ too)

beta (alkalosis): secret HCO3, absorb H+

22
Q

acidosis vs alklosis

A

acid:: <7.35 pH
alk: >7.45 pH

23
Q

respiratory acidosis

examples

A

pCO2 >45

hypoventilate –> decrease pH

not exhaling CO2 enough

apnea, obstructive lung disease, V/Q mismatching, right to left shunts

24
Q

respiratory alkalosis

examples

A

pCO2 <35mmHg

hyperventilate –> increase pH

anxiety, aspirin, stroke, oxygen deficiency from high altitude or pulmonary disease

25
Q

metabolic acidosis causes

A

low pH from loss of HCO3- or cant deal with new non-volatile acids

kidney failure to excrete metabolic acids (renal tubular acidosis)

form excess metabolic acids (diabetes, lactic acid)

add metabolic acids via ingestion (aspirin)

loss of base from body fluids (diarrhea, vomit)

26
Q

metabolic alkalosis examples

A

increase HCO3- (or loss of H+)

diuretics, excess aldosterone, vomit, alkaline drugs

27
Q

how do kidneys and lungs response if respiratory or metabolic alkalosis/acidosis

A

i.e. if respiratory acidosis then kidneys will excrete more H+

respiratory alkalosis then kidneys increase HCO3- excretion

ie. if metabolic acidosis alveolar hyperventilate so kidneys can increase H+ excretion

28
Q

anion gap

A

normal: 10-12mmol/L

from Cl- and HCO3-

to find cause of metabolic acidosis –> if too high might have too many acids i.e. ketones, lactate, salicylates (conjugate bases)

29
Q

causes of normal and high anion gap for metabolic acidosis

A

normal: diarrhea, renal tubular acidosis

high: uremia, lactic acid, ketoacidosis, methanol intoxication

30
Q

where is most of potasium

A

intracellular

dont want extraceullarl

31
Q

what 5 things shift potassium into cells

A

insulin (via Na+/K+ pump post prandial)

aldosterone

beta-adrenergic stimulation (NE, and epinephrine increase NA/K pump)

acid base imbalance (alkalosis shift K+ into cells, acidosis is out)

hyperkalemia (an increase in K+ in ECF shifts in via na/k pump)

32
Q

what 3 things shift potassium out of cell

A

cell lysis
strenuous exercise
increase ECF osmolarity
acidosis

33
Q

early vs late K+ shifts

A

early: Na/K pump activated by insulin, catecholamines, aldosterone

late: aldosterone via principal cells for renal excretions of K+

34
Q

PCT absorbption of k+

35
Q

K+ handing in tubules

A

reabsorb 90%

in distal nephron secrete it depending on aldosterone and pH

36
Q

K+ in PCT

A

reabsorb, link to Na+ and H2O (solvent drag)

ROMK channel on basolateral side
–> via decerase intraceullar ATP
–> also increase Na/K pump

37
Q

K+ in thick ascending limb

A

K+ and Cl- permeable, paraceullar

NKCC channel pull K+ into cell

38
Q

K+ in dif parts of tubule

A

PCT- ROMK channel

thick ascending limb: NKCC channel

distal: principal cells and alpha itnercalated, ENaC channel

39
Q

K+ in distal nephon

A

principal cells

aldosterone fro K+ secretion and Na+ reasborb

increase ENaC, Na/K pump

KCC transporter

alpha intercalated cells rescue K+ if hypokalemia
(H+/K+ countertrasnport via ATP, alkalosis)

40
Q

potassium excretory region

A

Most daily variation in potassium excretion is
caused by changes in potassium secretion in
distal convoluted tubule and early collecting
tubules/ducts

41
Q

cells for K+ and Na+ fine-tune via aldosterone

A

principal cells in collecting duct

also alpha intercalated cells

42
Q

luminal Na+ and K+ secretion

A

principal cells

secrete K+ and absorb Na+ in collecting duct

43
Q

increase K+ secretion

A

osmotic diuresis, volume
expansion, loop diuretics

44
Q

Na+ depreviation

A

increases aldosterone secretion

but K+ doesnt drop that much because theres decreasedGFR –> slow tubular fluid delivery –> less K+ washout

45
Q

pH impacting K+

A

alkalosis increases K+ transport into cells (hypokalemia)

acute acidosis reduced K+ secretion, but chronic increases it