week 3 lec 1 Flashcards

1
Q

atopic dermatitis causes

A

familial transmitted skin disease
–> genetic, immune, environmental risk factors

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2
Q

when does atopic dermatitis usually begin

A

childhood

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3
Q

hallmark symptom of atopic dermatitis

A

itch (can effect sleep, cause excoriations and infections)

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4
Q

co-morbidities with atopic demaitits

A

allergic asthma and allergic rhinitis

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5
Q

major features of atopic dermatitis

A

chronic or chronically relapsing

  • Pruritus
  • Eczematous dermatitis (acute, subacute, or chronic) -
  • Facial and extensor involvement in infancy
  • Flexural eczema or lichenification in children and adults
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6
Q

atopic dermatitis is commonly associate qith

A

family history of atopy (allergic rhinitis, Asthma, AD)

xerosis or skin barrier dysfunction

IgE reactivity

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7
Q

pathogenesis of atopic dermatitis is driven by

A

skin barrier defect (FLG gene)

environment

altered immunologic responses in T cells, antigen processing, inflammatory cytokines, host defense proteins, allergen sensitivity, and infection.

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8
Q

types of atopic dermatitis

A

eczema

contact dermatitis

seborrheic dermatitis

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9
Q

atopic dermatitis etiology

A

3x prevalence since 1960s

more in industrialized countries 10-20%

more females

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10
Q

acute lesion characteristics in a topic dermatitis

A

erythematous papulovesciles (small red bumps or blisters)

surface changes; pinpoint crusting or weeping from exudation

sxz: prurits –> itchy

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11
Q

subacute or chronic lesions in atopic dermatitis

A

dry, scaly plaques

excoriation and lichenification

pruritis, less erythema than acute

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12
Q

atopic dermatitis lesions

A

can have acute and chronic lesions

multiple areas

indistinguishable clinically or historically from other eczematous conditions like allergic contact dermatitis and nummular dermatitis

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13
Q

patients with darker skin and atopic dermatitis

A

follicular accentuation

flat-topped papule in lichenified areas

hyperpigmentation

(rare) vitiligo like depigmentation

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14
Q

infancy - which area on body for atopic dermatitis

A

face, scalp, extensors

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15
Q

adolescent with atopic dermatitis is usually on which area

A

flexural folds
(lichenification and chronic)

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16
Q

adults primary location for atopic dermatitis

A

hand eczema

also more flexural and around neck

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17
Q

1/3 of patients with atopic dermatitis have features of _____ deficiency

A

filaggrin deficiency

–> ichthyosis vulgaris, keratosis pilaris, and hyperlinear palms.

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18
Q

other features in atopic dermatiits

A

allergic shiners below eyes

conjunctivitis

elevated IgE

facial pallor

etc

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19
Q

comorbidities of atopic dermatitis

A

Th2 immune activation

high IgE

eosinophilia

predisposed to other allergies

psychosocial (anxiety/depress)

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20
Q

complications of atopic dermatitis

A

Bacterial, Viral, Fungal infections

Ocular Issues (eyelid dermatitis, etc)

Hand dermatitis

Exfoliative dermatitis

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21
Q

what is exfoliative dermatitis (complication of AD)

A

generalized (90% of body) redness, scaling, weeping, crusting, systemic toxicity, lymphadenopathy, and fever. Although this complication is rare, it is potentially life threatening

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22
Q

skin barrier function decreased in atopic dermatitis

A

-downregulate cornified enveloped genes (keratin, filaggrin, loricrin)

-reduced ceramide levels

-increase endogenous proteolytic enzyme activity

-enhance trans-epidermal water loss

scratching and exogenous proteases (ie. dust mites and s. aureus) impact barrier too

increase allergen absorption

lack endogenous protease inhibitors

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23
Q

soap and detergents effect skin barrier in AD

A

raise pH, increase endogenous protease activity –> further breakdown of epidermal barrier function

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24
Q

genes in atopic dermatitis

A

loss of function in filaggrin (chromosome 1q21)

–> effect barrier function: increase trans-epidermal water load, increase allergen and chemical entry, inflammatory response

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25
Q

filaggrin mutation in

A

Atopic dermatitis but also can be in normal people and be fine

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26
Q

3 characterizations of AD lesions

A

non-lesional AD

acute AD lesions (3 or fewer days after onset),

chronic skin lesions (>3 days’ duration)

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27
Q

non lesiona atopic dermatitis immunopathology

A

mild epidermal hyperplasia (thicken)

sparse perivascular T cell infiltrate in dermis

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28
Q

acute lesions in atopic dermatitis immunopathology

A

epidermal spongiosis (intracellular edema in epidermis disrupts keratinocytes)

T cell activation and skin-homing cutaneous lymphocyte- associated antigen (CLA)

sometimes mast cells (degranulation), eosinophils, basophils, neurophils

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29
Q

nonlesional and lesion atopic dermatitis have which dendritic antigen presenting cells

A

langerhan cells

inflammatory dendritic epidermal cells

macrophages

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30
Q

difference between non lesional and lesional atopic dermatitis for dendritic cells

A

non lesional has dendritic cells with fewer surface bound IgE

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31
Q

chronic lichenified lesions in atopic dermatitis

A

hyperplastic epidermis with elongation of rate ridges

hyperkeratosis

minimal spongiosis (reduced intercellular edema)

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32
Q

immune cells in chronic lichenified lesions of atopic dermatitis

A

increase langerhans cells with IgE

macrophages dominante dermis

mast cells

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33
Q

are there neutrophils and eosinophils in atopic dermatitis

A

no neutrophils - even if staph aureus infects

eosinophils: increased

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34
Q

allergic inflammation in atopic dermatitis from

A
  • cytokine and mediator secretion

-ROS intermediates

-toxic granule proteins

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35
Q

atopic dermatitis epidermis vs normal epidermis differences

A

-thymic stromal lymphopoeitin (TSLP) increased in AD which activates dendritic cells and cause Th2 response

IL-33 released by damaged keratinocytes causes type 2 immunity

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36
Q

normal keratinocytes lack something that is seem in atopic dermatitis and cause inflammation

A

Normal keratinocytes lack significant levels of TSLP and IL-33

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37
Q

TSLP and IL-33 is secreted by _____ and induces _____ to have a _____ response

A

secreted by epithelial cells

induce dendrite cells to drive Th0 cells into Th2

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38
Q

cytokines in nonlesional and acute atopic dermatitis

A

IL-4, IL-5, IL-13, IL-25, IL-31, IL-33.

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39
Q

cytokines in all stages of atopic dermatitis

and what cells their secreted by

A

type 2 cytokines

innate lymphoid type 2 cells
mast cells
basophils

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40
Q

key functions of type 2 cytokines in atopic dermatitis

A

Elevated IgE responses.

Eosinophilia.

Skin barrier dysfunction.

Allergic skin inflammation

Itching.

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41
Q

other cytokines outside of Th2 in atopic dermaatitis

A

IL-22, IL-17, IL-4, IL-13: inhibit terminal keratinocyte differentiation and inhibit filaggrin expression

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42
Q

IL-22, IL-17, IL-4, IL-13: inhibit what

A

inhibit terminal keratinocyte differentiation and inhibit filaggrin expression

IL-4 and IL-13 also enhance IL-23 from dendritic cells

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43
Q

IL-23 in atopic dermatitis is from what cell and enhances what

A

Dendritic cell–derived IL-23 enhances IL-22–IL-17 differentiation.

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44
Q

when going from acute to chronic atopic dermatitis what cytokines and IL’s increase

A

Th1 cytokine increases with IFN-y

IL-5 (promotes eosinophil survival)

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45
Q

TSLP in early atopic dermatitis

A

can be detected in infants before AD onset

TSLP-Th2-ILC2 pathway initiates AD

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46
Q

which chemokine are in atopic dermaitits

A

CTACK (CCL27)

CCR4 and CCL17

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47
Q

CTACK (CCL27) chemokine in atopic dermatitis

A

Attracts skin-homing CLA+ CCR10+ T cells into the skin.

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48
Q

CCR4 and CCL17 chemokine in atopic dermatitis

A

CCR4, expressed on skin-homing CLA+ T cells, binds to CCL17 on cutaneous venules.

Facilitates targeted T-cell migration into inflamed skin.

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49
Q

CCR4 expressing Th2 cells are recruited by what in AD

A

Macrophage-derived chemokine (MDC).

Thymus and activation-regulated cytokine (TARC).

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50
Q

severity of atopic dermatitis is correlated with

A

levels of thymus and activation-regulated cytokine (TARC).

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51
Q

Th1 cell migration in chronic AD is driven by which upregulated keratinocyte chemokines

A

Fractalkine

IFN-γ–inducible protein 10 (IP-10)

Monokine induced by IFN-γ (MIG)

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52
Q

immune cell infiltration in AD from which elevated CC chemokine

A

Macrophage chemoattractant protein-4 (MCP-4)

Eotaxin

RANTES (regulated on activation, normal T-cell expressed and secreted)

Recruit macrophages, eosinophils, and T cells into skin lesions.

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53
Q

immune effects on epithelial differentiation complex (in AD)

A

dry skin increases transepidermal water loss

minority of patient have filaggrin null mutations

most patients have immune mediated reduction in epidermal differentiation

activate proteases and lipases

defective epidermal barrier

allergens and microbes can penetrate

54
Q

effects of reduced epidermal differentiation in atopic dermatitis

A

Decreased production of:

Epidermal structural proteins.

Filaggrin breakdown products.

Epidermal lipids.

Antimicrobial peptides (AMPs).

55
Q

cytokines that downregualte filagrin expression in AD

A

TSLP, IL-4, IL-13

synergistic cytokines to IL-4 and IL-13: IL-17, IL-22, IL-25, and IL-33

56
Q

minority and majority of patients with AD have which changes that cause pathophysiology

A

minority of patient have filaggrin null mutations

most patients have immune mediated reduction in epidermal differentiation

57
Q

most prominent feature of AD

A

pruritus (itch)

58
Q

pruritus in AD is worse after

A

allergen exposure, humidity changes, excessive sweating, low concentrations of irritants

59
Q

pruitits in AD and mechanical injury from scratching can induce

A

pro- inflammatory cytokine and chemokine release

60
Q

what can cause pruritis in AD possibly?

A

histamine from mast cell

but H1 antihistamines dont help

topical corticosteroid and calcineurin inhibitor help so histamine may play a role

61
Q

molecules implicated in pruritis

A

histamine

IL-31 (cytokines from T cells)

stress-induced neuropeptides (substance P, CGRP)

proteases (act on protease-activated receptors; PAR-2)

eicosanoids (lipid mediator from arachidonic acid- i.e. prostaglandins, leukotrienes)

eosinophil derived proteins (major basic protein, eosinophil derived neurotoxin)

activate sensory nerves and inflame

62
Q

allergic contact dermatitis is a cell mediated ______ reaction

A

hypersensitivity; Type IV (delayed)

63
Q

what triggers allergic contact dermatitis

A

skin contact with environmental allergen

64
Q

how does allergic contact dermatitis work

A

immune system exposed to allergen

allergen/ hapten binds skin proteins and forms complex = foreign

sensitization leads to T cell activation and inflammatory response upon subsequent exposure

(prior sensation required)

65
Q

clinical presentation of allergic contact dermatitis

A

eczematous dermatitis

red, itch, swell, vesicles

chronic: lichenifcation and scaling

66
Q

acute phase of allergic contact dermatitis sx

A

where area was exposed:

pruritus
erythema
edema
vesicles

67
Q

chronic allergic contact dermatitis with repeated exposure to allergens causes lichenified erythematous plaques with

A

Hyperkeratosis.

Fissuring.

Pigmentary changes.

Lesions may spread beyond areas of direct exposure.

68
Q

key features of allergic contact dermatitis

A

itch and swell

69
Q

diagnose allergic contact dermaitits

A

patch testing is gold standard

70
Q

treat allergic contact dermatitis

A

avoid allergen

provide alternatives

71
Q

examples of allergens for allergic contact dermatitis

A

nickle, nail polish, fragrance, cosmetics, textiles, rubber related chemicals, adhesives, …

72
Q

allergic contact dermatitis presentation

A

pruritic, eczematous dermatitis @ site of allergen

geometric, linear or focal patterns (i.,e. poison ivy is linear)

73
Q

acute vs subacute vs chronic lesions in allergic contact dermatitis

A

acute: edema, erythema, vesicles or papules

subacute: rupture vesicle ooze, scaly juicy papule with weeping and crusting

chronic: scale, fissure, lichenification

74
Q

**SLIDE 57 chart for different types of allergic contact dermatitis

A

pigmented ACD on clothed portions of body from textile dyes and fragrances

i.e. eryhtema multiform like ACD on site of allergen from woods, medicament

etc

75
Q

allergic contact dermatitis is what type of hypersensitivity rxn

A

type IV; delayed

76
Q

features of allergic contact dermatitis

A

Erythema, edema, and papulovesiculation.

77
Q

2 phases of allergic contact dermatitis

A
  1. sensitization phase
  2. elicitation phase
78
Q

sensitization and elicitation phase of allergic contact dermatitis

A

sensitization: initial contact with allergen activates immune system to develop memory

elicitation: subsequent re-exposure triggers a complex inflammatory rxn

79
Q

what are haptens? how do they become activated in allergic contact dermatitis?

A

unprocessed allergen that need modification to become immunogenic

form hapten-protein complex –> hapten penetrate skin barrier and bind epidermal carrier proteins –> forms complete antigen that can trigger immune response

80
Q

how is the innate immune system activated in allergic contact dermatitis?

what cytokines do keratinocyte release when theres hapten exposure?

A

IL-1, IL-8, IL-18

TNFalpha

GM-CSF

81
Q

which cells uptake the hapten protein complex (“antigen”) and express on surface bound to HLA in allergic contact dermatitis

A

Langerhans cells (LCs) and dermal dendritic cells

82
Q

APC migration in allergic contact dermatitis

A

migrate via lymphs

present HLA-antigen complex (with hapten) to naive T cell

Naive T cell differentiate into memory T cells which expand clonal and aqeuire skin-specific homing antigens and go into circulation and act on cells with antigen in future exposure

83
Q

sensitization phase in allergic dermatitis lasts how long and sx

A

The sensitization phase lasts 10–15 days and is typically asymptomatic

84
Q

elicitation phase in allergic contact dermatitis

A

subsequent exposure to an allergen that the px is already sensitized to

inflam + TLR activation + nucleotide binding oligomerization domain like receptors

leads to neutrophil then T cell recruitment

85
Q

what happens when T cells get recruited in allergic contact dermatitis

A

antigen specific effector T cells go to skin

interact with APCs (PCs and dendritic cells) around post capillary venules

86
Q

Inducible Skin-Associated Lymphoid Tissue (iSALT) in allergic contact dermatitis elicitation phase

A

induced by inflammation

87
Q

what do T cells release to amplify the immune response in allergic contact dermatitis

A

IFN-y
TNF-a

which then recruit more inflammatory cells, stimulate macrophages, and keratinocytes to release more cytokines

88
Q

what suppresses the allergic contact dermatitis elicitation phase

A

regulatory T cells

Las can help develop reg T cells

89
Q

if px presents with eczematous dermatitis they might have

A

allergic contact dermatitis

90
Q

allergic contact dermatitis misconceptions

A

not alway bilateral even if antigen exposure is bilateral

even if exposure to allergen is uniform the eczematous manifestation can still be patchy

ACD can effect palms and soles

91
Q

irritant dermatitis if from

A

wet-to-dry cycling in cold seasons

92
Q

what is a predisposition to allergic and atopic dermatitis

A

Innate immune signals from irritant dermatitis

93
Q

how to help irritant dermatitis

A

decrease duration and frequency of irritant contact

while allergic contact dermatitis needs complete avoidance

94
Q

what helps irritant dermatitis

A

emollients

but emollient on normal skin over long periods of time can cause irritant dermatitis

hardening and sx disappear with continued irritate exposure without treatment

95
Q

what is it called when sx disappear in irritant dermatitis and prevent progression to allergic or atopic dermatitis

96
Q

when does irritant dermatitis manifest and resolve vs allergic contact dermatitis

A

irritant dermatitis manifests within hours of the causative exposure, and resolves within days of cessation of exposure.

In contrast, allergic contact dermatitis may manifest days after exposure and persist for weeks

97
Q

dermatitis definition

A

inflammatory disruption of the epidermis related to physical or immunologic provocation.

98
Q

dermatitis and ____ are used interchangeably

99
Q

histology of dermatitis

A

spongiosis

100
Q

dermatitis in general had impairment in what

A

barrier functions of the skin, which results in increased transepidermal water loss.

101
Q

irritant dermatitis from

A

bacteria, yeast, chemicals

combination of factors

I,e, detergent, rubber gloves, candida, malassezia, food, staph

102
Q

irritant dermatitis is caused by

A

physical damage to epidermis

103
Q

severe irritant dermatitis have what type of keratinocytes

A

necrotic keratinocytes

104
Q

variables influencing irritant dermatitis

A

climate and season, occlusion, frequency of exposure to the irritant, and concentration of the irritant

105
Q

common irritants for irritant dermatitis

A

solvents including water

detergents

disinfectants

anti-wrinkle medicaments

106
Q

risk factors for irritant dermatitis

A

wet work, detergents, gloves occlusion, occupation, water occlusion, solvents

107
Q

3 steps in irritant dermatitis pathogenesis

A
  1. epidermal insult
  2. danger signals
  3. potential fro allergic sensitization
108
Q

atopic dermatitis from what mutatitons

109
Q

kids will get irritant dermatitis in which areas (which leads to allergic contact dermatitis usually)

A

flexural areas

110
Q

step 1- epidermal insult in irritant dermatitis

A

solvents diminish epidermal barrier and remove lipids or desiccate stratum corneum from repeated rapid drying

genetic- filaggrin mutations lowers threshold to minor friction damaging epidermis

111
Q

step 2- danger signals in irritant dermatitis

A

damaged keratinocytes release alarmins (danger signals) (i.e. defensins and uric acid)

alarmins bind TLRs and promote inflammatory pathways like nuclear factor-KB that links to adaptive immune response and leads to allergic dermatitis

112
Q

step 3- potential for allergic sensitization in irritant dermatitis

A

chemical allergens and microorganism stimulate innate immune system and TLRs

predispose to allergic contact dermatitis

113
Q

complications of irritant dermatitis

A

food allergy

allergic contact dermatitis

114
Q

seborrheic dermatitis features and location

A

greasy, scaling patches and plaques appear on scalp, face, ears, chest, and intertriginous areas.

115
Q

cause of seborrheic dermatitis

A

abnormal immune mechanism, Malassezia, sebaceous glands, and individual susceptibility.

116
Q

who is seborrheic dermatitis most common in

A

adolescent and young adults from hormones increasing sebaceous gland activity

also > 50yoa

3 month of infants

-more in males
-seasonal (cold and dry)
-sun may decrease severity

117
Q

causes of seborrheic dermatitis

A

many….

sebaceous glands

immune status (I.e. AIDS and Parkinson, immunosuppressed)

malassezia (antifungals)

seasonal changes and sun

118
Q

immune system in seborrheic dermatitis

A

defective CD4+ and CD8+ T cells caused SD in mice

increase IgA and IgG

decrease B cells

increase NK cells

119
Q

which cytokines in seborrheic dermatitis

A

IL-1α, IL-1β, IL-4, IL-12, tumor necrosis factor-α, and interferon (IFN)- γ

histamine

120
Q

dandruff in seborrheic dermatitis

A

induced inflammatory genes and repressed lipid metabolism genes

oxidative stress

121
Q

microbe for seborrheic dermatitis

A

malassezia globosa and malassezia restricta

Pityriasis versicolor and Pityrosporum folliculitis, both induced by Malassezia, are often associated with SD

122
Q

key features of malazzesia in seborrheic dermatitis

A

lipase and phospholipase enzymes that make them lipophilic

these enzymes metabolize lipids into fatty acids for fungal cell wall formation

123
Q

what enzymes in malassezia and their effect in seborrheic dermatitis

A

lipase and phosphilpase contribute to

invading skin layers

dissemination across affected areas

124
Q

malassezia furfur effects in seborrheic dermatitis

A

disrupt protective skin barrier and induce inflammation

125
Q

malassezin is made by which malassezia and does what in serborrheci dermatitis

what does this receptor do

A

Malassezin, generated by M. furfur or M. restricta, acts as an agonist to the aryl hydrocarbon receptor (AhR).

AhR: differentiate Th-17 cells and mediate contact sensitivity (TH-17)

126
Q

what does aryl hydrocarbon receptor (AhR) via malassezia do in seborhhersic dermatitis

A

differentiate Th-17 cells

127
Q

what can cause dandruff in susceptible individuals

A

Oleic acid, produced by Malasseiza globosa lipase

128
Q

hyper or hypo proliferation in seborrheic dermatitis>

A

increased epidermal turnover; hyperproliferation

129
Q

DDX for seborrheic dermatitis

130
Q

seborrheic dermatitis treatment

A

here are case reports that keratolytics and antiinflammatory medications were successful in the treatment of the patients with SD whose treatment with amphotericin B had failed.

131
Q

what might alter epidermis and cause hyperproliferation in seborrheic dermatitis

A

increased activity of calmodulin