Week 4 - Bacteria and Biofilm Flashcards
What are the oral habitats/surfaces
- lips, cheeks, palate
- Tongue
- Teeth
Explain lips, cheek and palate as a oral habitat
They are:
- Shedding surfaces meaning that they don’t tend to build up a lot of bacteria as they shed the bacteria along with the bacteria constantly
- Keratinised - the hard palate is keratinised which makes it quite difficult for bacteria to attach there so there is only a certain types of bacteria that grow on the hard palate
Explain the tongue as a oral habitat
- Highly papillated
- Reservoir
Tongue is highly papillated (lots of bumps) where down in the low part of the papilla is a low oxygen environment where the bacteria can hide away in there and then replenish the rest of the mouth when they need to acting as a reservoir for certain types of bacteria
Explain the teeth as a oral habitat
- Number of surfaces
- Gingival crevice
- Non-shedding surface
- Plaque build up
Teeth is a
non-shedding surface which is why plaque can build up, so bacteria attaches and
bacteria attaches upon that.
How does bacteria adhere to surfaces
Adherence to a habitat in the oral cavity is due to interactions between a molecule that is found on the bacterium called the adhesion and a molecule on the host surface called a ligand, usually some sort of receptor, which interacts in a lock and key interaction.
What are the main oral microbiota
- Streptococci
- Veillonella
- Gram-positive Diptheroids (Corynebacterium)
- Gram-negative anaerobic bacilli
What are biofilms
Biofilms are free floating bacteria attached to a solid surface which contains multiple species of bacteria.
They are embedded within an extracellular polysaccharide slime layer which acts as a form of protection as its hard for antibiotics to get through
When nutritional needs re not met in the environment the biofilm will go into a latent state, where the bacteria cell’s aren’t dividing. Cell division will resume when sufficient nutrients are provides.
Where do biofilms form
- plaque on teeth
- Around dental implants
- Contaminated waterlines
What is dental unit water biofilms
Biofilm which forms where water is left to settle in the tubing and ridges of dental equipment.
This contamination with biofilm poses major issues:
- Infection risk (e.g. pseudomonas)
- Equipment breakdown from occluding the lines and also corroding metal components
What is pellicle
Pellicle are lots of glycoproteins in the saliva which settles on the teeth, its an automatic process and even after brushing they will resettle on the teeth.
Pellicle contains:
- mucins
- Salivary glycoproteins
- minerals
- immunoglobulins
- proline-rich proteins (PRPs)
Explain the steps for the development of plaque
- Pellicle - salivary glycoproteins attach to the tooth enamel,
- First pioneer bacteria species naturally present in the oral cavity, (streptococcus mutans, streptococcus sanguinis, Strep gordonii) adhere to the pellicle on the teeth enamel
- Once attached the bacteria starts to multiply and produces a slimy, sticky substances called extracellular polymeric substance (extracellular polysaccharide slime layer) which helps them adhere to each other and to the tooth, also has antibacterial characteristics
- Secondary colonisers which is more bacteria adheres to the biofilm and various species of bacteria coexist in a structured microbial community. Forms channels which enable the flow of oxygen and nutrients in, and the flow of waste out.
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- Overtime the plaque can mineralise and harden through the process of calcification. Calcium and phosphate ions from saliva contribute to the formation of a hard, mineralized deposit of the teeth known as dental calculus. Calculus provides a rough surface that makes it even easier for additional plaque to accumulate and attach to.
- If plaque is not adequately removed through oral hygiene practices, it can lead to inflammation of the gums, known as gingivitis. If left untreated, gingivitis can progress to periodontitis, a more severe form of gum disease that affects the supporting structures of the teeth
What are examples of first pioneer bacteria
streptococcus mutans,
streptococcus sanguinis,
Strep gordonii
What is the slime layer produced in the formation of plaque and what is its function
extracellular polysaccharide slime layer which helps them adhere to each other and to the tooth, also protectsit from antibiotics
What are the 3 types of subgingival plaque
- tooth-attached plaque
- Epithelial-attached plaque
- Unattached plaque (bacteria not apart of the biofilm)
What is the most dangerous type of subgingival plaque
tooth-attached plaque as it’s detrimental to periodontal tissue
What is calculus
Calculus is calcified plaque. It develops when dental plaque is not effectively removed through oral hygiene and they undergo calcification where minerals, primarily calcium and phosphate ions present in saliva, are deposited in the plaque. Overtime these minerals harden the plaque, transforming it into dental calculus.
Dental calculus has a rough porous surface which makes it even easier for bacteria to grow and adhere to it.
Dental calculus has to be removed by scaling instruments.
How is calculus removed
scaling instruments
What are the dangers of hard water areas
Hard water has a higher concentration of calcium and phosphate salts which causes the calcification of plaque