Week 2 - Microbiology Flashcards
What is a microorganism
An organism/replicating entity that is of microscopic size
What is microbiology
The study of micro-organisms (or macroscopic for example some worms) their relationships with humans
3 types of cellular microorganisms
Bacteria
Protozoa/other parasites
Fungi
2 types of acellular microorganisms
Viruses and prions (are more like deviated proteins)
5 features of prokaryotes
No membrane bound nucleus
Transcription and translation are coupled (i.e not in ribosome and nucleus)
Single, circular chromosomal DNA
Additional DNA carried in plasmids
Usually bacteria
4 features of eukaryotes
DNA is held in chromosomes inside membrane bound nucleus
Translation and transcription processes are compartmentalised
Cytoplasm is rich in membrane bound organelles
Cellular organisms other than bacteria
How is DNA found in bacteria
Long, circular form sometimes with plasmids in addition
What else surrounds the membrane around the bacteria
Cell wall
Thick capsule
How do bacteria reproduce
Via binary fission (asexual reproduction by separation into 2 bodies)
What makes bacteria gram-positive
Thick layer of peptidoglycan on cell wall so crystal violet remains
What makes bacteria gram-negative
Thin layer of peptidoglycan on cell wall
What makes bacteria gram-stain unreliable
Very small bacteria, atypical life cycle, atypical structure, don’t have complex wall
What can be used when bacteria are gram stain unreliable
PCR
Microscopy
Alternative staining/culture techniques
What counter-stains the gram negative bacteria
Safranin
2 examples of gram positive coccus bacteria
Streptococcus aureus
Streptococcus pneumoniae