Virology L7 & 1/2 8 Flashcards

1
Q

FOR RNA VIRUSES dont memorize the polarity “only know if they are ss or ds”

Retroviridae

Characteristics
§ Linear (+)sense ? or ds? RNA
§ shape? capsid
§ Envelope with ?-proteins
§ Release by ?

Transmission
§ ?-to-cell
§ ?
§ ? (e.g. Jaagsiekte)

** Pseudodiploid ? = 2 identical shape? (+)sense ss RNA strands **

Retro = reverse/backward
§ RNA is ?-transcribed to DNA-intermediates via ? (RT)
§ Retrovirus DNA is inserted in host genome as ?

A

FOR RNA VIRUSES dont memorize the polarity “only know if they are ss or ds”

Retroviridae

Characteristics
§ Linear (+)sense ss RNA
§ icosahderal capsid
§ Envelope with gag-proteins
§ Release by budding

Transmission
§ cell-to-cell
§ fluid
§ airborne (e.g. Jaagsiekte)

** Pseudodiploid genome = 2 identical linear (+)sense ss RNA strands ** (note: the strands aren’t connected to each other so they aren’t considered a double stranded virus)

Retro = reverse/backward
§ RNA is reverse-transcribed to DNA-intermediates via reverse transcriptase (RT)
§ Retrovirus DNA is inserted in the host genome as provirus

below IMP to understand:
PICTURE:
ENTRY: “entry” occurs through membrane fusion and then the uncoating step - release from capsid and then RNA virus moves to nucleus and will have replication of their genome following actions of the reverse transcriptase which makes the double-stranded intermediate

the double-stranded piece are integrated with the help of another enzyme called “integrase” which cut open the host genome literally and inserts viral genome into it

EXIT: during stress, RNA release occurs where RNA pol II (helps in proteolytic cleavage during virion maturation), another enzyme that helps in releasing that provirus
and then we have transcription and splicing thus, back to single-stranded RNA strands (until this happens in the nucleus)

then outside the nucleus, the virus is built and replication occurs and then proteins are made (such as Gag proteins and envelope proteins as seen in pic), and then all are assembled and released.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

IMP SLIDE! ! !

Retroviridae

Three important viral enzymes
§ ?
§ ?: integration of viral DNA into host’s genome
§ ?: proteolytic cleavage during virion maturation

§ ? cells are required for building in the genetic material
-> EXCEPT genus ?: can integrate in non-dividing host cells

§ Retroviridae infect a wide range of animals including humans
§ Associated with ? syndromes and cancer

§ Oncovirus = any virus with ? or ? genome causing ?
§ Proto-oncogene = gene that encodes for proteins regulating cell ? and differentiation and that can become an ? (gene potentially causing cancer)

A

IMP SLIDE! ! !

Retroviridae

Three important viral enzymes
§ reverse transcriptase
§ Integrase: integration of viral DNA into host’s genome
§ RNA pol II: proteolytic cleavage during virion maturation

§ active cells are required for building in the genetic material
-> EXCEPT genus Lentivirus: can integrate in non-dividing host cells

§ Retroviridae infect a wide range of animals including humans
§ Associated with immunodeficiency syndromes and cancer

§ Oncovirus = any virus with DNA or RNA genome causing cancer
§ Proto-oncogene = gene that encodes for proteins regulating cell growth and differentiation and that can become an oncogene (gene potentially causing cancer)

(note: smtms protogene transforms into protooncogene thus producing cancer; WHY we can pinpoint why cancer develops - many diff. rzns also included
altho they belong to the same family have diff. ways of replication strategies (so approach also differs)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

IMP SLIDE/pic !!

(GOOGLE: A proto-oncogene is a healthy gene found in the cell. Each proto-oncogene is responsible for making a protein involved in cell growth, division, and other processes)

§ Proto-oncogenes mistakenly incorporated into provirus

§ Disruption of cellular proto-oncogenes (e.g. viral promoter triggers overexpression)

ACUTELY transforming retrovirus:
- faster development of cancer cells
- infection of cells and integration of proto-oncogene
- infection of new host cell
- integration and expression of viral oncogene
(many virions are produced here and can affect many cells thus called acute and so cancer develops faster
the moment they build a provirus, they kind of cut the proto-oncogene into pieces and they take a part of that oncogene in their pro-virus, smtms happens randomly
As a result, you will have virus particles when this cell again releases its provirus (called ONCOGENE) and creates new variants,
you will have new variants that hold a little bit of that proto-oncogene in here

VIRAL ONCOGENE = provirus incorporating natural protooncogene
When these virions (blue ones) infect in a new host cell that will lead to the expression of the viral oncogene and to a transformation of that newly infected cell So the newly infected cell becomes a cancer cell!)

SLOWLY transforming retrovirus:
- just 1 here
- infection with retrovirus carrying promoter gene
- integration of promoter gene adjacent to proto-oncogene
- conversion of proto-oncogene to oncogene
(just 1 virion causing cancer so only 1 cancer cell; sometimes can take years)

(notes: here in slowly tran. retrovirus: virus (blue one) incorporates a promoter gene;
A promoter gene that’s kind of like an extra boost. Gene promotes the translation of certain genes. This is a viral genome that holds already promoter genes, and that is integrated next to the promoter oncogene.

So we have the proto-oncogene present in our genome and the provirus pushes itself next to it. And that provirus holds a promoter gene.
At this point, that promoter gene will induce the conversion of your natural proto-oncogene to an oncogene and that 1 cell becomes an infected cell.)

THEREFORE
SLOWLY TRAN. V. = 1 virus = 1 infected cell
ACUTELY TRAN V. = release of many diff. virions that infect many cells (1st infection ability to more quickly infect cells than slowly transforming virus)

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

AVIAN LEUKOSIS (look at slides for pics)

genus Alpharetrovirus
** species: Avian ? ? virus (ASLV or ALV) **
host: ?

§ ? leukosis and ? (blood cell tumor from lymphocytes)

A

AVIAN LEUKOSIS

genus Alpharetrovirus

** species: Avian sarcoma leucosis virus (ASLV or ALV) **
host: poultry

§ Lymphoid leukosis and lymphoma (blood cell tumor from lymphocytes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Enzootic nasal tumor

§ Genus ?,
species Enzootic ? ? virus (ENTV)

§ Host: sheep, ?

§ Tumors in the ? epithelium

Jaagsiekte (same genus as enzootic nasal tumor)

Genus Betaretrovirus,
species Jaagsiekte ? retrovirus (JSRV)

Host: sheep, ?
Contagious lung cancer or ovine pulmonary adenocarcinoma: ? distress when u chase animals

(massive sputum = characteristic of jaagsiekta (chasing)

A

Enzootic nasal tumor

§ Genus Betaretrovirus,
species Enzootic nasal tumor virus (ENTV)

§ Host: sheep, goat

§ Tumors in the nasal epithelium

Jaagsiekte

Genus Betaretrovirus,
species Jaagsiekte sheep retrovirus (JSRV)

Host: sheep, goats
Contagious lung cancer or ovine pulmonary adenocarcinoma: respiratory distress when u chase animals

(massive sputum = characteristic of jaagsiekta (chasing)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Bovine leukosis

§ Genus ?retrovirus,
species Bovine ? Virus
§ Host: ?
§ Enlargement of ? nodes (e.g. retro-ocular -> protrusion of ? membrane)

A

Bovine leukosis

§ Genus Deltaretrovirus,
species Bovine Leukemia Virus
§ Host: cattle
§ Enlargement of lymph nodes (e.g. retro-ocular -> protrusion of conjunctival membrane)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Equine Infectious Anemia, Swamp Fever

§ Genus ?virus,
species Equine ? ? virus

§ Host: ?
§ ?, thrombocytopenia, ?, ? loss, ? of legs
§ Mechanical vectors: stable ?!

A

Equine Infectious Anemia, Swamp Fever

§ Genus Lentivirus,
species Equine Infectious Anemia virus

§ Host: equidae
§ anemia, thrombocytopenia, fever, weight loss, swelling of legs
§ Mechanical vectors: stable flies!

(anemia - episodes (chronic) so is stable; can developed after several years; over have more and more and then more strong clinical signs
-flies can play a role as mechanical vector; viruses don’t have an active replication cycle inside the fly hence mechanical vector; ZIKA virus (active replication cycle in mosquitoes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Feline Leukemia (IMP SLIDE!!!)

§ Genus ?retrovirus, species Feline Leukemia Virus (FeLV)
§ Host: ?

§ Transmission: ** close contact with ? or ? secretions **, to a lesser extent through milk & blood
§ Infection in ? possible
§ ? carriers (0.5% of pet cats) -> risk to others!

A

Feline Leukemia

§ Genus gammaretrovirus, species Feline Leukemia Virus (FeLV)
§ Host: felids

§ Transmission: ** close contact with nasal or saliva secretions **, to a lesser extent through milk & blood
§ Infection in utero possible
§ asymptomatic carriers (0.5% of pet cats) -> risk to others! (can be transmitted vertically (offspring)!!

right pic:
early immune response = ABORTIVE INFECTION: effective immune response, replication halted, rarely diagnosed as infection gets cleared in local lymph nodes (includes going through oropharynx, then tonsils & then clears the infection at local lymph nodes)

late immune response = REGRESSIVE infection: effective immune response, transient viraemia, viral reactivation possible (bone marrow)
- here we see spread of virus in the blood thus “active viraemia”; from blood they can move to bone marrow where they can effect the precursors of your blood cells
- - here they can remain asymptomatic in regressive infection

poor/absent immune response = progressive infection: continuously viraemic, disease development, poor diagnosis
- However, what we do see is that there’s a large chunk of animals that can become more progressive and they will be continuously viraemic. at this point -> high loads of virus particles in the blood, but also secondary infections. And so you will not only have these clinical signs of leukemia, you will have the anemia, look scraggly (epithelial problems susceptible to bacterial infection)

(if a cat has FLV and asymptomatic then can ask the O to not let it be around other cats and monitor it closely and any other health issues to get an idea of which stage of FLV it might be in)

left pic: virus shed in the saliva and other bodily fluids of an infected cat

primarily transmitted from queens to kittens (some risk of transmission from prolonged contact or bite wounds)
- can lead to:
1. abortive infection

  1. regressive infection
    a. may remain asymptomatic for life b. can become progressive with inciting cause
  2. progressive infection -> will develop clinical signs
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Feline Leukemia

§ Clinical signs: ?, fever, ?, opportunistic ?, bone ? disorders
§ ? or in final stage ?
§ More progressive than ?
§ Vaccines and more intense testing -> decline of prevalence of ? infections

A

Feline Leukemia

§ Clinical signs: lethargy, fever, opportunistic infections, stomatitis, bone marrow disorders
§ immunosuppression or in final stage lymphomas
§ More progressive than FIV
§ Vaccines and more intense testing -> decline of prevalence of FeLV infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Feline immunodeficiency - IMP SLIDE!!!

§ Genus ? (same genus as equine infectious anemia virus),
species Feline ? Virus (FIV)
§ Host: ? (2.2-4.4% infected worldwide), endemic in African lions

§ Transmission: ** ? injuries (saliva) **
§ Virus infects CD4-T cells, CD8-T cells, B cells and macrophages -> depletion of CD4-T cells

§ Clinical signs: attack on ? system -> anemia, low ? blood cell count -> secondary infections, cancer, ? diseases (pic in Q)

Feline immunodeficiency
§ Diagnosis: detection of antibodies -> seropositive
§ Control: ? or ? cats (no need for ? – consensus USA)

A

Feline immunodeficiency

§ Genus Lentivirus (same genus as equine infectious anemia virus),
species Feline leukomia Virus (FIV)
§ Host: cats (2.2-4.4% infected worldwide), endemic in African lions

§ Transmission: ** BITE injuries (saliva) **
§ Virus infects CD4-T cells, CD8-T cells, B cells and macrophages -> depletion of CD4-T cells

§ Clinical signs: attack on immune system -> anemia, low white blood cell count -> secondary infections, cancer, neurological diseases (pic in Q)

Feline immunodeficiency
§ Diagnosis: detection of antibodies -> seropositive
§ Control: spaying or neutering cats (no need for euthanasia – consensus USA)

note: pics are imp!! go through them!

right pic shows HIV but FIV similar HIV
1. read

in the left pic:
1. virus shed in the saliva of an infected cat (note: in FeLV also in body fluids as well as in saliva)
2. primarily transmitted via a deep wound
3. asymptomatic
4. from asymptomatic -> either remain asymptomatic for life OR progression to clinical disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Rhabdoviridae

§ Characteristics
§ Linear (-)sense ss or ds RNA ?
§ ** IMP Helical ? **
§ Enveloped or non-enveloped?
§ ** ? -shaped **
§ Release by ?

§ Ribonucleoprotein complex: RNA bound by viral ?
§ ? body: eosinophilic, inclusion bodies found in the ? of infected nerve cells

§ Hosts: (in)vertebrates
§ Cell tropism: neurons -> ? virus

§ Transmission: animal bite -> ? (rabies)
§ Present in all continents except Antarctica and ?

A

Rhabdoviridae

§ Characteristics
§ Linear (-)sense ss RNA
§ ** IMP Helical nucleocapsid **
§ Enveloped
§ ** BULLET -shaped **
§ Release by budding

§ Ribonucleoprotein complex: RNA bound by viral nucleoprotein
§ NEGRI body: eosinophilic, inclusion bodies found in the cytoplasm of infected nerve cells

§ Hosts: (in)vertebrates
§ Cell tropism: neurons -> neurovirulent virus

§ Transmission: animal bite -> saliva (rabies)
§ Present in all continents except Antarctica and Australia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Rabies

§ Genus ?, species ? Virus
§ Reservoir: dogs, cats, wild ? & ?, bats, ?
§ Animal bite -> initial replication in myocytes -> transport through peripheral nerves -> central nervous sytem -> ** neuronal infection! **
§ ** Virus is shed in ? of infected animals **
§ fast or slow?-moving virus (~ distance bite to brain)
§ Incubation time: 3-8 days or weeks? in dogs

A

Rabies

§ Genus Lyssavirus, species Rabies Virus
§ Reservoir: dogs, cats, wild canids & fields, bats, mongooses
§ Animal bite -> initial replication in myocytes -> transport through peripheral nerves -> central nervous sytem -> ** neuronal infection! **
§ ** Virus is shed in the saliva of infected animals **
§ slow-moving virus (~ distance bite to brain)
§ Incubation time: 3-8 weeks in dogs

(virus NOT found in blood!! as they are only in myocytes (so distance from origin of replication to CNS) so luckier to be bitten on toe than closer to CNS (can take weeks for it to reach CNS) however if closer to CNS then bad as it will reach CNS faster)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Rabies

§ After the virus reaches brain, three phases are defined:

§ ? Phase (2-3 days): nervousness, anxiety, solitude, different behavior (friendly -> irritable; aggressive -> affectionate). Licking of the ? side is mostly observed.

§ ? Phase (1-7 days): ?, irritable, hyperresponsive to ? and auditory stimuli, roaming, ?.

§ ? Phase: ? (inability to swallow), deep labored breathing, ? facial muscles, ? failure and death

Rabies
- direct fluorescent antibody test: look for presence of rabies virus ? in brain tissue
- not in the ?!!!

A

Rabies

§ After the virus reaches brain, three phases are defined:

§ prodomal Phase (2-3 days): CHANGE IN BEHAVIOUR; nervousness, anxiety, solitude, different behavior (friendly -> irritable; aggressive -> affectionate). Licking of the bite side is mostly observed.

§ furious Phase (1-7 days): restless, irritable, hyperresponsive to visual and auditory stimuli, roaming, disorientated.

§ paralytic Phase: salivate (inability to swallow), deep labored breathing, paralyzed facial muscles, respiratory failure and death

Rabies
- direct fluorescent antibody test: look for presence of rabies virus antigens in brain tissue
- not in the BLOOD!!!
- ZOONOTIC!!!

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

IMP SLIDE!!

Orthomyxoviridae

Characteristics
§ Segmented ? (-)sense ss RNA
(remember!! SEGMENTED. - has a big devlopement on how they develop, replicate etc.)
§ Enveloped with ** ? **
§ Release by ?

§ Glycoprotein spikes
§ Neuraminidase = NA = N
§ Haemaglutinin = HA = H

Three genera are defined by antigenic differences in nucleoprotein and matrix protein:
§ Influenza virus A: humans, birds, ? (pandemics!)
§ Influenza virus B: humans, ?
§ Influenza virus C: humans, ?, dogs

International nomenclature of infuenza viruses

A

IMP SLIDE!!

Orthomyxoviridae

Characteristics
§ Segmented linear (-)sense ss RNA
(remember!! SEGMENTED. - has a big devlopement on how they develop, replicate etc.)
§ Enveloped with ** spikes **
§ Release by budding

§ Glycoprotein spikes
§ Neuraminidase = NA = N
§ Haemaglutinin = HA = H

Three genera are defined by antigenic differences in nucleoprotein and matrix protein:
§ Influenza virus A: humans, birds, mammals (pandemics!) - influenza A most common one
§ Influenza virus B: humans, seals
§ Influenza virus C: humans, pigs, dogs

pic: THGSYS (virus type, host of origin (if humans no host of origin), geographic origin, strain number, year of isolation, virus subtype

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Flu epi - & pandemics

  • influenza A subtypes are defined by H & N ?
  • Multiple strains within a subtype

regular epidemic and pandemic (don’t memorize the years)

  • Reassortment = mixing of ? material of species into new ?, resulting in a ? virus
A

Flu epi - & pandemics

  • influenza A subtypes are defined by H & N virus
  • Multiple strains within a subtype

regular epidemic and pandemic (don’t memorize the years)

  • Reassortment = mixing of the genetic material of species into a new combination, resulting in a reassortment virus (can include different species)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Flu epi- & pandemics IMP SLIDE!!!!

Antigenic ? = small changes in the genes that happen continually over time as the virus replicates (Influenza A, Influenza B)
§ Closely related viruses share ? properties -> ?
§ HOWEVER when accumulation of changes over time -> then immune system does no longer recognize virus (renew influenza vaccines!)

§ Antigenic ? = abrupt, major genetic changes in Influenza A viruses, resulting in new HA and/or new NA proteins -> new Influenza A subtype

A

Flu epi- & pandemics IMP SLIDE!!!!

Antigenic drift = small changes in the genes that happen continually over time as the virus replicates (Influenza A, Influenza B)
§ Closely related viruses share antigenic properties -> cross-protection
§ HOWEVER when an accumulation of changes over time -> then the immune system does no longer recognize the virus (renew influenza vaccines!)

§ Antigenic shift = abrupt, major genetic changes in Influenza A viruses, resulting in new HA and/or new NA proteins -> new Influenza A subtype

17
Q

Swine flu

§ Influenza A virus (common or rare??) & Influenza C virus (rare or common??)
§ Influenza B virus has not been reported
§ Transmitted via ? contact (virus survives up to 3 months in pigs)

Prevent spread to humans: vaccination of pigs & ? control

intestinal: diarrhea

respiratory: coughing, sore throat

psychological: lethargy, lack of appetite

nasopharynx: sneezing, mucous: nose/eye

systemic: fever, weight loss and poor growth

A

Swine flu

§ Influenza A virus (common) & Influenza C virus (rare)
§ Influenza B virus has not been reported
§ Transmitted via direct contact (virus survives up to 3 months in pigs)

Prevent spread to humans: vaccination of pigs & sanitary control

intestinal: diarrhea

respiratory: coughing, sore throat

psychological: lethargy, lack of appetite

nasopharynx: sneezing, mucous: nose/eye

systemic: fever, weight loss and poor growth

18
Q

Filoviridae

Characteristics
§ Linear (-)sense ss or ds? RNA
§ Pleomorphic or ** ? **
§ shape? nucleocapsid
§ Enveloped with ?

§ Reservoir: ? bats; occasional humans, ?
§ Geography: Central Africa and South Asia
§ Transmission: contact with body ?

§ Cytopathic infection (i.e. ? changes) in ? cells and in ? organs of the host

Ebola
§ Genus Ebolavirus
§ Haemorrhagic fever (an escape of blood from a ruptured blood vessel), fatality rate 50-90%
§ Infection of ? -> release of cytokines -> ? injury -> ?
§ Ebola outbreak 2014-2016 (Individual humanity!)
- ZOONOTIC!!!

Marburg haemorrhagic fever
§ Genus ?
§ ? fever in humans and non-human primates
§ ? all over the body

Who is at risk?
§ ? staff, ?, travelers
§ Close contact with ? bats and ?-human primates
§Transmission from animal to human: bat ? and ?
§Transmission from human to human: direct contact with ? fluids

  • RARE or COMMON? HUMAN DISEASE!
  • prevention:
    1. barrier ? techniques = prevent direct contact
    2. awareness
A

Filoviridae

Characteristics
§ Linear (-)sense ss RNA
§ Pleomorphic or ** filamentous **
§ helical nucleocapsid
§ Enveloped with spikes

§ Reservoir: reservoir bats; occasional humans, primates
§ Geography: Central Africa and South Asia
§ Transmission: contact with body spikes

§ Cytopathic infection (i.e. structural changes) in cultured cells and in target organs of the host

Ebola
§ Genus Ebolavirus
§ Haemorrhagic fever (an escape of blood from a ruptured blood vessel), fatality rate 50-90%
§ Infection of monocytes -> release of cytokines -> endothelial injury -> haemorrhage
§ Ebola outbreak 2014-2016 (Individual humanity!)
- ZOONOTIC!!!

Marburg haemorrhagic fever
§ Genus Marburgvirus
§ haemorrhagic fever in humans and non-human primates
§ Bleeding all over the body

Who is at risk?
§ Hospital staff, vets, travelers
§ Close contact with fruit bats and non-human primates
§Transmission from animal to human: bat faeces and aerosols
§Transmission from human to human: direct contact with body fluids

  • RARE HUMAN DISEASE!
  • prevention:
    1. barrier nursing techniques = prevent direct contact
    2. awareness
19
Q

Overview of DNA & RNA viruses

REOVIRIDAE
Characteristics
§ ** shape? ss or ds RNA **
§ ? capsid
§ Non-enveloped or enveloped?

§ Transmission: fecal-oral route

§ Hosts: (in) ?

“Respiratory ? ?” (note REO in reoviridae stands for this)
§ Tropism for mucosal ? and ? cells

§ Disease: ? tract disease, ?

BLUETONGUE (reoviridae)
- Genus ?, species Bluetongue virus
- Host: ?
- Transmission: ? bite
- Acute disease with high ? and mortality in susceptible breeds, but not ?
- Torticollis -> “? disease” (occurrence of tilting of head (neurological) thus called ? disease)

Viral Diarrhea
- Genus ? virus, different species
- Host: ?, infants, ? of many animals
- Transmission: ?-oral
- Economic losses due to rota viral enteritis in intensively-reared piglets and calves

A

Overview of DNA & RNA viruses

REOVIRIDAE
Characteristics
§ ** linear ds RNA **
§ double capsid
§ Non-enveloped

§ Transmission: fecal-oral route

§ Hosts: (in) vertebrates

“Respiratory Enteric Orphan” (note REO in reoviridae stands for this)
§ Tropism for mucosal gut and respiratory cells

§ Disease: respiratory tract disease, gastroenteritis

BLUETONGUE (reoviridae)
- Genus Orbivirus, species Bluetongue virus
- Host: sheep
- Transmission: arthropod bite
- Acute disease with high morbidity (diseases) and mortality in susceptible breeds, but not contagious
- Torticollis -> “dancing disease” (occurrence of tilting of head (neurological) thus called DANCING disease)

Viral Diarrhea
- Genus Rota virus, different species
- Host: children, infants, young of many animals
- Transmission: fecal-oral
- Economic losses due to rota viral enteritis in intensively-reared piglets and calves

20
Q

PARAMYXOVIRIDAE

Characteristics
§ Linear (-)sense ss or ds? RNA or DNA?
§ Non-enveloped or enveloped?
§ Release by ?

§ Transmission: ? of small ?

§ Hosts: ?, humans

Difference between orthomyxo- and paramyxoviruses! (they look similar tho)
§ Orthomyxo: segmented genome allows for ?
§ ** IMP !! Paramyxo: ? genome, one ? -> long-term immunity **

(serology: the scientific study or diagnostic examination of blood serum, especially with regard to the response of the immune system to pathogens)

Epidemiology
§ Humans: ?, ?
§ Animals: Canine ?, ?, ? disease

A

PARAMYXOVIRIDAE

Characteristics
§ Linear (-)sense ss RNA
§ Enveloped
§ Release by budding

§ Transmission: inhalation of small droplets

§ Hosts: vertebrates, humans

Difference between orthomyxo- and paramyxoviruses! (they look similar tho)
§ Orthomyxo: segmented genome allows for antigenic shift
§ ** IMP !! Paramyxo: non-segmented genome, one serotype -> long-term immunity **

(serology: the scientific study or diagnostic examination of blood serum, especially with regard to the response of the immune system to pathogens)

Epidemiology
§ Humans: mumps, measles
§ Animals: Canine distemper, Rinderpest, Newcastle disease

21
Q

PARAMYXOVIRIDAE examples

CANINE DISTEMPER
§ Genus ?, species Canine ? virus
§ Hosts: ? (puppies!!), ?, wolves, foxes, ?, skunks, ? and other wild carnivores
§ Transmission: ? (through sneezing or coughing)
§ Attacks ?, ? & ? system
§ Symptoms: ?, loss of ?, vomiting, ? discharge, coughing, ? behaviour, muscle ?, seizures, ?
§ Hardening of foot pads -> “hard ? disease” (neurological sign more common; hyper keratinization)
§ ? is essential for prevention!
(no cure for disease)

(convulsions: a sudden, violent, irregular movement of a limb or of the body, caused by involuntary contraction of muscles and associated especially with brain disorders such as epilepsy, the presence of certain toxins or other agents in the blood, or fever in children:)

A

CANINE DISTEMPER
§ Genus Morbillivirus, species Canine distemper virus
§ Hosts: dogs (puppies!!), coyotes, wolves, foxes, ferrets, skunks, raccoons and other wild carnivores
§ Transmission: airborne (through sneezing or coughing)
§ Attacks respiratory, gastrointestinal & nervous system
§ Symptoms: fever, loss of appetite, vomiting, nasal discharge, coughing, circling behavior, muscle twitches, seizures, convulsion
§ Hardening of foot pads -> “hard pad disease” (neurological sign more common; hyperkeratinization)
§ Vaccination is essential for prevention!
(no cure for disease)

  • canine distemper is asymptomatic, has a pattern of symptoms and neurological disease
    (similar to epileptic seizures)
22
Q

PARAMYXOVIRIDAE (another example)

Rinderpest
§ Genus ?, species Rinderpest virus
§ “can wipe out a cattle herd in a matter of days”
§ 3D’s: Dead, ?, ?

§ Host: ?
§ ? virus emerged from rinderpest as a zoonotic disease between the 11th and 12th centuries.
§ Global ?! (2011)-stamping out & ?

Newcastle Disease
§Genus ?, species ? disease virus
§ Hosts: ? and other ? species
§ Transmission: ? discharges and ?
§ ? bird disease (domestic and wild avian species): acute ? disease, with ?, ? manifestations, or ? in the clinical form (e.g. tremors, torticollis, swelling of tissue)
? egg production and misshapen eggs

§ Control: Vaccination and ? measures

A

(note genus is same as distemper)

PARAMYXOVIRIDAE (another example)

Rinderpest
§ Genus morbillivirus, species Rinderpest virus
§ “can wipe out a cattle herd in a matter of days”
§ 3D’s: Dead, discharge, and diarrhea

§ Host: cattle
§ Measles virus emerged from rinderpest as a zoonotic disease between the 11th and 12th centuries.
§ Global eradication! (2011)-stamping out & vaccination

Newcastle Disease
§Genus Avulavirus, species Newcaastle disease virus
§ Hosts: poultry and other bird species
§ Transmission: respiratory discharges and feces
§ Contagious bird disease (domestic and wild avian species): acute respiratory disease, with depression, nervous manifestations, or diarrhea in the clinical form (e.g. tremors, torticollis, swelling of tissue)
Reduced egg production and misshapen eggs

§ Control: Vaccination and misshapen measures

all 3 examples of paramyxomiridae = have vaccination for them!

23
Q

RNA viruses

Coronaviridae
Characteristics
§ Linear (+)sense ss or ds? RNA
§ Enveloped with “ ** ? **” (petal-shaped spikes)
§ Release by ?

§ Hosts: ?, humans

Transmission:
§ ? of small droplets
§ ?-oral route

§ Primary site of infection: ? cells of respiratory or ? tract

§ Epidemiology
§ Feline ? coronavirus (FECV) & Feline ? ? virus (FIPV)
§ Transmissible ? virus swine (TGEV)

A

RNA viruses

Coronaviridae
Characteristics
§ Linear (+)sense ss RNA
§ Enveloped with “ ** PEPLOMERS **” (petal-shaped spikes)
§ Release by exocytosis

§ Hosts: vertebrates, humans

Transmission:
§ inhalation of small droplets
§ fecal-oral route

§ Primary site of infection: epithelial cells of the respiratory or intestinal tract

Epidemiology
§ Feline enteric coronavirus (FECV) & Feline infectious peritonitis virus (FIPV)
§ Transmissible gastroenteritis virus swine (TGEV)

blue ones are virion particles being released in the pic

24
Q

CORONAVIRIDAE

Feline infectious peritonitis (FIP) virus - FIPV

§Genus ?coronavirus, species ? coronavirus (FCoV)

Two forms of the virus
§ Feline ? Coronavirus (FECV)
§ Feline ? Peritonitis virus (FIPV)

§ Transmission: ?-oral (catteries, animal shelters!) -> highly ?
§ Hosts: ?

A

CORONAVIRIDAE

Feline infectious peritonitis (FIP) virus - FIPV

§Genus alphacoronavirus, species feline coronavirus (FCoV)

Two forms of the virus
§ Feline enteritis Coronavirus (FECV)
§ Feline infectious Peritonitis virus (FIPV)
(- can see more of feline enteric coronavirus (but when it mutates it can become feline infectious p v.)
- feline enteric coronavirus: has a predilection for enterocytes, given in name

predilection: a preference or special liking for something)

§ Transmission: fecal-oral (catteries, animal shelters!) -> highly contagious
§ Hosts: Felidae

25
Q

IMP SLIDE!!!

FIP
FECV mutates in body and becomes FIPV
- FIPV has tropsim for macrophages (the turning of all or part of an organism in a particular direction in response to an external stimulus).

  • asymptomatic carriers shed the virus in faeces
  • FIPV is incurable and fatal

(notes:
- v regularly transmitted from cat to cat through the fecal-oral route.
- shed from faeces but can’t see it clinically
- often have asymptomatic carriers of feline enteric coronavirus, so it maintains itself in that cat population.

  • problem is smtms the virus undergoes mutation and thus it changes its tropism from intestinal cells to macrophages
  • and it is this change in tropism that will lead to a different clinical pattern; unlike feline enetero virus, FIPV can be seen
  • one of the most common infectious diseases in v young cats and is in the stage is INCURABLE and fatal.
A
26
Q

FIP (1-3%)
- resistant (5-10%)
- transient infection (70%) mild diarrhea
- persistent carrier (5-10%)

(And so a lot of asymptomatic infections or transient infections with your feline, in theory, corona virus, transient infections, self-limiting diarrhea - this is a self-limiting infection - DONT TREAT IT WITH ANTIBOITICS!

so animals will overcome this however persistent carriers, they are a problem because the resistant carriers, they will not show clinical signs, but they will have a continuous excretion of high loads of these virus particles.

So they will be continuously leading to a high infection pressure in the environment.
So if you have a couple of these in the cattery, that’s not good bc then you have a higher chance of an individual developing FIP.

A
27
Q

Feline infectious peritonitis (FIP)

Dry or non-effusive FIP
§ Loss of ?
§ ? loss
§ ? inside eyes
§ ? color change
§ 15% ? signs

Wet or effusive FIP
§ Damaged and leaking ?
§ ?
§ ? breathing

A

Feline infectious peritonitis (FIP)

Dry or non-effusive FIP
§ Loss of weight
§ appetite loss
§ yellowing inside eyes
§ iris color change
§ 15% neurological signs

Wet or effusive FIP
§ Damaged and leaking blood vessels
§ ascites (build-up of fluid in the abdomen)
§ Difficult breathing due to mechanical pressure from the diaphragm due to ascites

TO PREVENT THIS - PRACTICE GOOD HYGIENE

28
Q

CORONAVIRIDAE

SEVERE ACUTE RESPIRATORY SYNDROME (SARS)

  • Genus ?, species ? coronavirus
  • initial flu-like symptoms: fever, ?, ? pain followed within 14 days by ? symptoms e.g. dyspnea, pneumonia
  • not recorded in ? before outbreak in China end of 2002

COVID-19
- Genus ?, species ? (SARS-CoV-2)
- Hosts: humans, some ?
- not recorded in humans before outbreak in China in dec 2019
- transmission via ? and indirect contact with ?-contaminated materials

  • coronavirus variants due to gene ? are not new nor unexpected
  • mild symptoms: fever, cough
  • severe symptoms: shortness of breath, ?, heart and ? failure, death
A

CORONAVIRIDAE

SEVERE ACUTE RESPIRATORY SYNDROME (SARS)
- Genus betacoronavirus, species SARS coronavirus
- initial flu-like symptoms: fever, headache, muscle pain followed within 14 days by respiratory symptoms e.g. dyspnea, pneumonia
- not recorded in humans before outbreak in China end of 2002

COVID-19
- Genus Betacoronavirus, species SARS coronavirus 2 (SARS-CoV-2)
- Hosts: humans, some animals
- not recorded in humans before outbreak in China in dec 2019
- transmission via droplets and indirect contact with droplet-contaminated materials

  • coronavirus variants due to gene mutations are not new nor unexpected
  • mild symptoms: fever, cough
  • severe symptoms: shortness of breath, pneumonia, heart and kidney failure, death

covid has also spread to animals however their covid hasn’t been spread to humans