Unit 7 - review questions

Question 1

An organism with growth that is inhibited in the absence of oxygen is described as?

Answer 1

Facultative anaerobes

Question 2

An organism with growth that is enhanced in the presence of low oxygen tension is described as?

Answer 2

Microaerophilic

Question 3

An organism with growth that requires high levels of carbon dioxide is described as?

Answer 3

Capnophilic

Question 4

Most pathogenic bacteria are?

Answer 4

Mesophiles

Question 5

Rod-shaped bacteria are?

Answer 5

baccili

Question 6

Bacteria can be arranged in a “Chinese letter” pattern known as?

Answer 6

Palisades

Question 7

A type of culture media that contains additives that allow certain microorganisms to grow while inhibiting the growth of others is?

Answer 7

Selective

Question 8

A type of culture media that contains additives that detect certain biochemical reactions of the bacteria is?

Answer 8

Differential

Question 9

Which media is recommended for culture and sensitivity testing?

Answer 9

Mueller-Hinton

Question 10

Pathogenic bacteria exhibit ____ hemolysis on blood agar.

Answer 10

Beta-

Question 11

Acid-fast stains are used primarily for the identification of -__.

Answer 11

Mycobacterium

Question 12

For best results, microbiology samples must be collected?

Answer 12

aseptically

Question 13

The most common cause of diagnostic failure with microbiologic specimens is?

Answer 13

specimen contamination during collection

Question 14

The first step in collecting samples for dermatophyte testing is?

Answer 14

cleaning the area to minimize surface contamination

Question 15

Bacterial samples from hollow organs are usually collected by?

Answer 15

aspiration

Question 16

An example of an area that is likely to contain a single organisms is?

Answer 16

urine collected by cytocentesis

Question 17

The primary stain component for most Gram staining kits is?

Answer 17

Crystal violet

Question 18

The mordant component for most Gram staining kits is?

Answer 18

iodine

Question 19

The counter stain component for most gram staining kits is?

Answer 19

Safranin

Question 20

When Gram stain results are ambiguous, the ____ procedure is performed.

Answer 20

KOH

Question 21

The stain that is primarily used to detect Mycobacterium and Nocardia species is?

Answer 21

Ziehl-Neelsen

Question 22

The stain that is primarily used to detect spirochetes and rickettsiae is?

Answer 22

Giemsa

Question 23

Fluorescent stains are primarily used for the identification of?

Answer 23

Pseudomonas

Question 24

A bacterial culture that is used for endospore staining should be approximately ___ hours old.

Answer 24

48

Question 25

The technique that is designed to isolate pure cultures of bacteria is the?

Answer 25

Quadrant streak method

Question 26

Routine cultures are incubated for ___hours before initial examination.

Answer 26

18

Question 27

The purpose of the candle jar is to?

Answer 27

increase carbon dioxide levels

Question 28

Terms that describe the form of a colony on a plate include?

Answer 28

Rhizoid

Question 29

Agar slant tubes are usually inoculated with the?

Answer 29

slant s streak

Question 30

Culture and sensitivity tests are evaluated by measuring the?

Answer 30

zone of inhibition

Question 31

The preferred culture media for performing the sensitivity test is?

Answer 31

Mueller-Hinton

Question 32

The application of undiluted urine directly to the culture and sensitivity test plate is referred to as the?

Answer 32

direct sensitivity

Question 33

Inoculating the culture and sensitivity test plate with colonies from a pure culture is referred to as the ___ method.

Answer 33

indirect sensitivity

Question 34

The numbers of colony forming units on a culture plate of a sample of urine collected by cystocentesis are considered significant when more than ____ such units are present per milliliter of urine.

Answer 34

1000

Question 35

Which test requires the use of a microscope slide with a concave area?

Answer 35

Hanging drop motility test

Question 36

Kovac's reagent is used to determine whether bacteria produce_____.

Answer 36

Indole

Question 37

Hydrogen peroxide is the reagent for the ___ test.

Answer 37

Catalase

Question 38

A positive catalase test is indicated by the production of?

Answer 38

bubble on the slide

Question 39

If the milk/reagent mixture in the California Mastitis test thickens immediately, with some gel formation, the result is recorded as?

Answer 39

distinct positive

Question 40

Most fungi are composed of structures called?

Answer 40

Hyphae

Question 41

The culture media that is most often used for the growth of fungi and yeasts is?

Answer 41

Sabouraud agar

Question 42

A useful stain for the evaluation of fungal organisms is?

Answer 42

Lactophenol cotton blue

Question 43

The preferred method for the testing for dermatophytes is to?

Answer 43

pluck some hairs from the periphery of the lesions and perform a culture

Question 44

Culture specimens from Microsporum or Trichophyton should be collected from?

Answer 44

the periphery of the lesion