Unit 7 - review questions Flashcards

1
Q

An organism with growth that is inhibited in the absence of oxygen is described as?

A

Facultative anaerobes

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2
Q

An organism with growth that is enhanced in the presence of low oxygen tension is described as?

A

Microaerophilic

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3
Q

An organism with growth that requires high levels of carbon dioxide is described as?

A

Capnophilic

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4
Q

Most pathogenic bacteria are?

A

Mesophiles

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5
Q

Rod-shaped bacteria are?

A

baccili

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6
Q

Bacteria can be arranged in a “Chinese letter” pattern known as?

A

Palisades

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7
Q

A type of culture media that contains additives that allow certain microorganisms to grow while inhibiting the growth of others is?

A

Selective

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8
Q

A type of culture media that contains additives that detect certain biochemical reactions of the bacteria is?

A

Differential

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9
Q

Which media is recommended for culture and sensitivity testing?

A

Mueller-Hinton

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10
Q

Pathogenic bacteria exhibit ____ hemolysis on blood agar.

A

Beta-

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11
Q

Acid-fast stains are used primarily for the identification of -__.

A

Mycobacterium

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12
Q

For best results, microbiology samples must be collected?

A

aseptically

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13
Q

The most common cause of diagnostic failure with microbiologic specimens is?

A

specimen contamination during collection

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14
Q

The first step in collecting samples for dermatophyte testing is?

A

cleaning the area to minimize surface contamination

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15
Q

Bacterial samples from hollow organs are usually collected by?

A

aspiration

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16
Q

An example of an area that is likely to contain a single organisms is?

A

urine collected by cytocentesis

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17
Q

The primary stain component for most Gram staining kits is?

A

Crystal violet

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18
Q

The mordant component for most Gram staining kits is?

A

iodine

19
Q

The counter stain component for most gram staining kits is?

A

Safranin

20
Q

When Gram stain results are ambiguous, the ____ procedure is performed.

A

KOH

21
Q

The stain that is primarily used to detect Mycobacterium and Nocardia species is?

A

Ziehl-Neelsen

22
Q

The stain that is primarily used to detect spirochetes and rickettsiae is?

A

Giemsa

23
Q

Fluorescent stains are primarily used for the identification of?

A

Pseudomonas

24
Q

A bacterial culture that is used for endospore staining should be approximately ___ hours old.

A

48

25
Q

The technique that is designed to isolate pure cultures of bacteria is the?

A

Quadrant streak method

26
Q

Routine cultures are incubated for ___hours before initial examination.

A

18

27
Q

The purpose of the candle jar is to?

A

increase carbon dioxide levels

28
Q

Terms that describe the form of a colony on a plate include?

A

Rhizoid

29
Q

Agar slant tubes are usually inoculated with the?

A

slant s streak

30
Q

Culture and sensitivity tests are evaluated by measuring the?

A

zone of inhibition

31
Q

The preferred culture media for performing the sensitivity test is?

A

Mueller-Hinton

32
Q

The application of undiluted urine directly to the culture and sensitivity test plate is referred to as the?

A

direct sensitivity

33
Q

Inoculating the culture and sensitivity test plate with colonies from a pure culture is referred to as the ___ method.

A

indirect sensitivity

34
Q

The numbers of colony forming units on a culture plate of a sample of urine collected by cystocentesis are considered significant when more than ____ such units are present per milliliter of urine.

A

1000

35
Q

Which test requires the use of a microscope slide with a concave area?

A

Hanging drop motility test

36
Q

Kovac’s reagent is used to determine whether bacteria produce_____.

A

Indole

37
Q

Hydrogen peroxide is the reagent for the ___ test.

A

Catalase

38
Q

A positive catalase test is indicated by the production of?

A

bubble on the slide

39
Q

If the milk/reagent mixture in the California Mastitis test thickens immediately, with some gel formation, the result is recorded as?

A

distinct positive

40
Q

Most fungi are composed of structures called?

A

Hyphae

41
Q

The culture media that is most often used for the growth of fungi and yeasts is?

A

Sabouraud agar

42
Q

A useful stain for the evaluation of fungal organisms is?

A

Lactophenol cotton blue

43
Q

The preferred method for the testing for dermatophytes is to?

A

pluck some hairs from the periphery of the lesions and perform a culture

44
Q

Culture specimens from Microsporum or Trichophyton should be collected from?

A

the periphery of the lesion