Topic 1 - Rate Calculations For Enzyme Activity Flashcards
What is the formula used for rate calculations in enzyme activity?
Rate = Change / Time
This formula helps determine how fast an enzyme is working.
In the context of enzyme activity, what does ‘Change’ refer to?
‘Change’ refers to the change in the substance being measured, such as the enzyme
In this case, it is the amount of substrate that is converted.
What does ‘Time’ represent in the rate calculation for enzyme activity?
‘Time’ refers to the time taken for the change to occur
It is the duration over which the enzyme activity is measured.
What type of enzyme is a protease?
A protease is an enzyme that breaks down proteins
Proteases catalyze the hydrolysis of peptide bonds in proteins.
If 5g of protein is completely used up in 30 minutes by protease enzymes, what is the rate of reaction?
Rate = 5g / 30 minutes = 5g / 0.5 hours
The rate of reaction quantifies the efficiency of the enzyme.
Fill in the blank: The rate of reaction can be calculated using the equation Rate = Change / _______.
Time
True or False: The rate of reaction is independent of the amount of substrate used.
False
The rate of reaction depends on the amount of substrate being converted.
What is the rate at which the enzyme catalyzes the reaction?
10g / hour
What factors may change the enzyme’s reaction rate?
Temperature, pH, substrate concentration