Topic 1 -Enzymes : Mechanisms,z Denaturation And Factors Affecting Enzyme Activity Flashcards

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1
Q

What are enzymes?

A

Biological catalysts that increase the rate of reaction without being used up

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2
Q

What roles do enzymes play in reactions?

A

They can break up large molecules and join small ones

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3
Q

What type of molecules are enzymes?

A

Protein molecules

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4
Q

Why is the shape of an enzyme important?

A

The shape of the enzyme is vital to its function

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5
Q

What is the active site of an enzyme?

A

The uniquely shaped area where the substrate binds

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6
Q

What does the Lock and Key Hypothesis explain?

A

How enzymes work by matching the shape of the substrate to the active site

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7
Q

What forms when a substrate binds to an enzyme?

A

An enzyme-substrate complex

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8
Q

What must happen for enzymes to catalyze reactions?

A

They must bind to a substrate with a complementary shape

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9
Q

What is enzyme specificity?

A

The ability of enzymes to only catalyze reactions with substrates that fit their active site

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10
Q

What environmental conditions do enzymes require for optimal activity?

A

Optimum pH and temperature, as well as optimum substrate concentration

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11
Q

Fill in the blank: Enzymes can only catalyze reactions when they bind to a substrate that has a _______.

A

complementary shape

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12
Q

What is the optimum temperature range for humans?

A

Around 37 degrees Celsius

This temperature varies in other organisms.

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13
Q

What happens to the rate of reaction as temperature increases up to the optimum?

A

It increases

Above the optimum temperature, the reaction rate rapidly decreases and can eventually stop.

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14
Q

What occurs when the temperature becomes too hot for an enzyme?

A

The bonds holding the enzyme together begin to break

This leads to denaturation.

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15
Q

What is the result of an enzyme being denatured?

A

The active site changes shape, preventing substrate binding

The enzyme can no longer function.

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16
Q

What is the optimum pH for most enzymes?

A

7 (neutral)

Some enzymes produced in acidic conditions have a lower optimum pH.

17
Q

What happens to enzymes if the pH is too high or too low?

A

The forces holding the amino acid chains are affected

This can lead to denaturation.

18
Q

What is the relationship between substrate concentration and the rate of reaction?

A

The rate of reaction increases with substrate concentration up to a saturation point

Beyond this point, increasing substrate concentration has no effect.

19
Q

What is the saturation point in enzyme activity?

A

The point at which increasing substrate concentration no longer affects the rate of reaction

The saturation point varies for different enzymes.