TISSUE TYPES- EPITHELIA Flashcards

1
Q

Describe the basic stages of tissue section preparation

A
1. Tissue collection
Fixation (10% formalin and trimming)
Dehydration (series of alcohols increasing conc to 100%)
Paraffin embedding
Sectioning with microtome (3-4 micrometers)
Straitening sections on water bath
Transferring sections to a slide
Staining and cover slipping
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2
Q

What does Haematoxylin stain

A

(Blue)

Stains mainly genetic material

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3
Q

What does eosin stain

A

PINK

Stains mainly proteins (mainly cytoplasm)

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4
Q

What are the types of simple epithelium

A

Simple squamous epithelium
Simple cuboidal epithelium
Simple columnar epithelium
Simple pseudostratifed epithelium

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5
Q

What are the types of Stratified epithelium

A

Stratified squamous epithelium
Stratified columnar epithelium
Stratified cuboidal epithelium
Transitional epithelium

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6
Q

Where is simple squamous epithelium found

A
Alveoli
Vessels (called endothelium)
Body cavities ( called mesothelium)
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7
Q

What does simple squamous epithelium look like and what is it best adapted for. Disadvantage of it

A

Single thin layer of cells. Cells are very flat
Best suited for passive transport of substances across the cytoplasm.
Provide little protection and thus are not found on surfaces subjected to high stress

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8
Q

Where are simple cuboidal cells found

A
Thyroid
Kidney 
Lung
Ovary
Ducts and secretory portions of many glands
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9
Q

Where are simple columnar cells found

A

Intestine
Female reproductive tract
Many exocrine glands

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10
Q

What do simple cuboidal cells look like and what do they do

A

Are a single layer of cells
Aren’t as long as simple columnar cells
Usually associated with secretion and /or absorption

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11
Q

What do simmple columnar cells look like and what do they do

A

A single layer of cells
Are longer than simple cuboidal cells
Usually associated with secretion and / or absorbtion

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12
Q

What does simple pseudostratified epithelium look like and what does it do

A

Looks like multiple layers of cells but nuclei aren’t in rows
Secretion and movement of particles along tubular organs

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13
Q

Where is simple pseudostratified epithelium found

A

Trachea
Bronchi
Epididymis
Van deferens

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14
Q

Where is stratified squamous epithelium found

A

Covers most of the external and some internal body parts

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15
Q

Where are stratified cuboidal and columnar epithelia found

A

Found in areas of transition between simple and stratified epithelial
Respiratory tract
Ducts of exocrine glands

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16
Q

Where is transitional epithelium found

A

Only in the urogenital system ( ureter, renal pelvis )

17
Q

What are endocrine glands

A

Ductless glands that have no connections o external or internal surfaces
Produce hormones
Are near blood vessels
High diversity of morphology

18
Q

What are exocrine glands

A

Glands that secrete its product into a lumen or free space

19
Q

What specialisations does epithelium have

A

Basal membrane
Apical modifications
Cell junctions

20
Q

What are examples of apical modifications

A

Cilia - movie , long cell processes
Microvilli - no-motile , minute projections found in the epithelia specialised for absorption
Stereocilia - long microvilli

21
Q

What are the different types of cell junctions

A

Adherent junctions
Tight junctions
Gap junctions

22
Q

What are adherent junctions

A

Anchoring

Cells must bind to each other and to connective tissue to assure tissue cohesion

23
Q

What are tight junctions

A

Occluding
Involved in the control of what enters the body
Control diffusion of nutrients from intestinal lumen to blood vessels
Limit the paracellular movement of water

24
Q

What are gap junctions

A

Communicating
Cell must communicate with one another
Are aqueous channels allowing smal molecules to pass between two adjacent cells