Thrush- Tumor Immunology Flashcards

1
Q

cancer cells are ____ self cells. they may express diff markers and have abnormal growth control. (lack of apoptosis, and p53 mutations)

A

altered

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2
Q

what is a malignant cancer cell? what is metastasis

A

when cancer cells invade other cells and go where they are not supposed to be

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3
Q

what are the different mutations

A
  1. chemical carcinogens; nitrates, asbestos, nicotine/tar
  2. physical carcinogens; UV light (sun), radiation
  3. viruses; Human T cell lymphotrophic virus 1 (HTLV-1), Epstein Barr virus (EBV), hepatitis virus, human papillomavirus (HPV)
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4
Q

Because of the mutations, cancers are:

A
  1. complex genetic disease
  2. heterogenous; constant changes and evasion of host control
  3. often has lack of antigenicity; derived from host
  4. metastatic; able to move form one location to another
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5
Q

how do you increase gene expression

A

move regulatory gene next to a diff promoter region.

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6
Q

____ gets expressed in certain situations and not. if moves to a gene that is always turned on, the gene constantly gets turned on, B cell proliferates and you get burst lymphoma (8–> 14). If you move cyme next to T cell receptor enhancer, it won’t be a problem. (usually silenced)

A

c-myc

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7
Q

colon cancer is due to_____mutations in a stepwise fashion. You make a mutation in a tumor suppressor gene, then the protein that was encoded by the gene is not working well then you get mutation in oncogene and other TSG’s until you get to the stage of metastatic cancer.

A

multiple

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8
Q

There is a variable immune response, Since ____ derived (self), tumors may not be recognized as foreign = no immune response. Also if immunosuppressed, increased incidence of cancer formation. Some tumors do have significant antigenic determinants and can be recognized as “altered.” –>melanomas (uv light), or virally induced tumors. Tumor infiltrating lymphocytes are usually suppressed

A

host

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9
Q

what are tumor antigens

A

Ag expressed only by the tumor, therefore they are abnormal/foreign antigens. Reality is there aren’t a lot of these out there.

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10
Q

what are tumor associated antigens

A

not unique to tumor cells but there is something abnormal about them. Normal proteins, but not normal proteins for an adult. (expressing moleucules at abnormal time in stage of development). may be proteins expressed at low levels in normal cells but much higher on the cancer cell.

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11
Q

what are the two categories of tumor antigens

A
  1. tumor specific

2. tumor associated antigens

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12
Q

what are oncofetal antigens

A

normal proteins exp during fetal dev but during cancer formation they get formed again. CEA is found in some colon cancers and these levels of marker proteins could be used to measure cancer burden

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13
Q

what are markers of oncofetal antigens

A
  1. Carcinoembryonic antigen (CEA): found in some colon cancers and also can be used to measure cancer burden.
  2. Alpha fetoprotein (AFP): increased in hepatitis or pregnancy. It can also mean they have cancer; found in patients with liver and germ cell tumors.
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14
Q

___ antigens; you can have viral proteins by expressed and presented with MHC class I.

A

viral;

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15
Q

what are the oncoviral proteins associated with?

A

HPV type 16, E6, and E7 proteins
Nautre of antigen: viral transforming gene products.
Tumor type: cervical carcinoma

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16
Q

surface immunoglobulins are associated with ____

A

lymphoma; specific IG after gene rearrangements in B cell clone.

17
Q

if tumor antigens, why no immune recognition

A

tumor related immune evasion

  1. lack of antigenic epitope; self cells (not exp # of foreign antigens)
  2. decreased MHC expression; Class I (unless b cell lymphoma); deficient antigen processing
  3. lack of costimulation: cells reduce levels of B7 (imp in activating T cells)
  4. produce inhibitory substances: such as cytokines
  5. shedding of tumor antigens: tolerance induction due to a certain protein
18
Q

How to you detect tumors

A
  1. radioimmunoassay, immunoelectrophoreis, ELISA of secretion/sera/urine for:
  2. myeloma proteins: look for B cell tumors
  3. oncofetal antigens: CEA or AFP
  4. alkaline phosphatase: prostate and liver cancer
  5. prostate specfic antigen (PSA): normal protein expressed at higher levels in prostate cancers
19
Q

You see increased level of CEA in some types of cancer or pathology. These antigens are tumor associated but just bc you see increased level it could also be _______ not just cancer.

A

pathological

20
Q

_____ moniters certain tumor markers on cells in the blood (flow cytometry) or in situ (microscopy)

A

immunoflourescnce

21
Q

How do you detect minimal disease to see if the tumor will come back in the future

A
  1. PCR: detects cancer genes witihin cell

2. changes in cytokine level:

22
Q

what are conventional therapy of cancer

A
  1. surgery
  2. radiotherapy
  3. chemotherapy
23
Q

what is passive immunotherapy

A
  1. monoclonal Ab to tumor specific antigens–> to cause ADCC and/or C’ activation
  2. monoclonal Ab to signal apoptosis–> Ab binding to Fas protein
  3. monoclonal Ab to prevent angiogenesis: tumors need to stimulate blood vessel formation if they are to grow beyond 1mm
24
Q

_____ is a major marker on B cell

A

CD20

25
Q

If you have Ab against Ag, Ab had to be gen in animal that rec protein as foreign. If you take mouse Ab and put it ind it will bind to cancer cells and kill them, but you can’t use mouse An amymore cause it will recognize antigen as foreign, You can _____ these Ab –> molecular attach human Fc region. Amount of mouse foreign molecule is reduced.

A

humanize

26
Q

_____ is where you stick something to other proteins. Where you deliver toxicity specific to cancer cells.

A

conjugate

27
Q

what is active immunotherapy

A
  1. tumor infiltrating lymphocytes: Isolate T cells,a ctivate with IL-2 and put them back in.
  2. Dendritic cells pulsed with tumor antigens: allows for better antigen processing/presentation and costimulation.
  3. Bone marrow transplant: can be useful for some tumors of lymphoid origin.
  4. cytokines: to activate an immune response (“break” suppression).
  5. Vaccination: Good for viral infection; papilloma virus vaccine for cervical cancer
28
Q

What are select FDA approved cytokines for cancer therapy

A
  1. IL-2: helps CD8 T cells and NK cells (altered self cells); get cells activated to prevent suppression and respond to cancer. (inc. vascular permeability)
  2. IFN-alpha: stimules better immune recognition by enhancing MHC expression along with Ag on the surface of the tumor cell.
29
Q

_____ is an antiviral cytokine

A

IFN-alpha

30
Q

future includes new tumor _____ as well as ____ antibodies

A

vaccines; bispecific