STAINS_Other Info

Question 1

Most sensitive technique for identifying DNA

Answer 1

In situ hybridization

Question 2

DNA amplification

Answer 2

PCR

Question 3

Pseudogout

Answer 3

Chondrocalcinosis

Question 4

Pigment removal

70% ETOH + 28% NH 3 water

Answer 4

Kardasewitsch method

Question 5

Remove yellow color of HNO3

Answer 5

0.1% urea + 5% NaSO4

Question 6

Most definitive of malignancy

Answer 6

Metastasis

Question 7

Most reliable indicator of prognosis of malignant tumors

Answer 7

Degree of localization

Question 8

Hgb = emerald green

Answer 8

Dunn-Thompson

Question 9

Removes excess melanin

Answer 9

K2 MnO4
H2 O2

Question 10

Contains formalin, K2 CrO 4 and HAc

Answer 10

Helly’s

Question 11

Fixative for CNS (gold/silver stain)

Answer 11

Formalin ammonium bromide

Question 12

Increased tissue shrinkage

Answer 12

Alcohol as 1’ fixative

Question 13

Not satisfactory for PAS

Answer 13

Glutaraldehyde

Question 14

Nonaqueous fixative

Answer 14

Carnoy’s

Question 15

Pheochromocytoma

Answer 15

Orth’s

Question 16

PTAH for cross-striations
Wash tissue in water after fixation in Zenker’s

Answer 16

Zenker’s

Question 17

Combines w/ amino group

Answer 17

Formaldehyde

Question 18

Nonadditive fixative

Answer 18

Ethanol

Question 19

Prevent brown microcrystalline deposits in H & E stain

Answer 19

pH >6.0 (formalin)

Question 20

Not easily decolorized w/ acidic solutions

Answer 20

Weigert’s hematoxylin

Question 21

Inherently acidic

Answer 21

Natural resins (mounting)

Question 22

Microincineration

Answer 22

Formalin-alcohol

Question 23

Substance that can bind silver but need a chemical reducer

Answer 23

Churukian-Schenk technique

Question 24

Substance that can both bind & reduce silver

Answer 24

Masson-Fontana

Question 25

Isopentane at -150’C If isopentane is low, dust muscle w/ talc then freeze in liquid nitrogen

Answer 25

Muscle biopsies

Question 26

Naphthol AS-D chloroacetate esterase

Answer 26

Paraffin sections

Question 27

Specimens may remain indefinitely

Answer 27

Zamboni’s PAF

Question 28

Glutaraldehyde

Specimens may be removed after…

Answer 28

2-4 hrs

Question 29

10% NBF

pH

Answer 29

7.2-7.4

Question 30

Pure polymer of formaldehyde

Answer 30

Paraformaldehyde

Question 31

Calibrate pH meter to 7.0

Answer 31

Warthin-Starry stain

Question 32

Criteria for the diagnosis of normal pregnancy

Answer 32

Marked progesterone effect
At least 50% of intermediate cells in clusters
At least some typical pregnancy cells present
<30% of matured superficial cells
Doderlein-filled dirty BG

Question 33

Chromatin stain

Answer 33

Carmine

Question 34

Best Carmine

Answer 34

Carmine + Aluminum chloride = For glycogen

Question 35

Mucicarmine

Answer 35

Carmine + Aluminum hydroxide = For C. neoformans and mucin

Question 36

Picrocarmine

Answer 36

Carmine + Picric acid = for neuropathological studies

Question 37

One of the most frequently applied for staging individual tumors

Answer 37

Duke’s staging for neoplasia of the rectum