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Histopath LEC > STAINS_Other Info > Flashcards

STAINS_Other Info Flashcards

1
Q

Most sensitive technique for identifying DNA

A

In situ hybridization

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2
Q

DNA amplification

A

PCR

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3
Q

Pseudogout

A

Chondrocalcinosis

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4
Q

Pigment removal

70% ETOH + 28% NH 3 water

A

Kardasewitsch method

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5
Q

Remove yellow color of HNO3

A

0.1% urea + 5% NaSO4

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6
Q

Most definitive of malignancy

A

Metastasis

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7
Q

Most reliable indicator of prognosis of malignant tumors

A

Degree of localization

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8
Q

Hgb = emerald green

A

Dunn-Thompson

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9
Q

Removes excess melanin

A

K2 MnO4
H2 O2

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10
Q

Contains formalin, K2 CrO 4 and HAc

A

Helly’s

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11
Q

Fixative for CNS (gold/silver stain)

A

Formalin ammonium bromide

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12
Q

Increased tissue shrinkage

A

Alcohol as 1’ fixative

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13
Q

Not satisfactory for PAS

A

Glutaraldehyde

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14
Q

Nonaqueous fixative

A

Carnoy’s

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15
Q

Pheochromocytoma

A

Orth’s

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16
Q

PTAH for cross-striations
Wash tissue in water after fixation in Zenker’s

A

Zenker’s

17
Q

Combines w/ amino group

A

Formaldehyde

18
Q

Nonadditive fixative

A

Ethanol

19
Q

Prevent brown microcrystalline deposits in H & E stain

A

pH >6.0 (formalin)

20
Q

Not easily decolorized w/ acidic solutions

A

Weigert’s hematoxylin

21
Q

Inherently acidic

A

Natural resins (mounting)

22
Q

Microincineration

A

Formalin-alcohol

23
Q

Substance that can bind silver but need a chemical reducer

A

Churukian-Schenk technique

24
Q

Substance that can both bind & reduce silver

A

Masson-Fontana

25
Q

Isopentane at -150’C If isopentane is low, dust muscle w/ talc then freeze in liquid nitrogen

A

Muscle biopsies

26
Q

Naphthol AS-D chloroacetate esterase

A

Paraffin sections

27
Q

Specimens may remain indefinitely

A

Zamboni’s PAF

28
Q

Glutaraldehyde

Specimens may be removed after…

A

2-4 hrs

29
Q

10% NBF

pH

A

7.2-7.4

30
Q

Pure polymer of formaldehyde

A

Paraformaldehyde

31
Q

Calibrate pH meter to 7.0

A

Warthin-Starry stain

32
Q

Criteria for the diagnosis of normal pregnancy

A

Marked progesterone effect
At least 50% of intermediate cells in clusters
At least some typical pregnancy cells present
<30% of matured superficial cells
Doderlein-filled dirty BG

33
Q

Chromatin stain

A

Carmine

34
Q

Best Carmine

A

Carmine + Aluminum chloride = For glycogen

35
Q

Mucicarmine

A

Carmine + Aluminum hydroxide = For C. neoformans and mucin

36
Q

Picrocarmine

A

Carmine + Picric acid = for neuropathological studies

37
Q

One of the most frequently applied for staging individual tumors

A

Duke’s staging for neoplasia of the rectum

Histopath LEC (37 decks)

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