Adhesives/ Floatation/ Drying

Question 1

Adhesives

Answer 1

• Mayer’s Egg Albumin
• Dried albumin
• Starch paste
• Plasma
• 1% Gelatin
• 1% Methyl Cellulose
• Poly-L Lysine
• Sodium silicate
• Resins

Question 2

Adhesives

Most common;
Never used with Pap Staining Method as it will retain OG stain;

Answer 2

Mayer’s Egg Albumin

Question 3

Composition of mayer’s egg whites

• -> main adhesive
• -› increases viscosity and prevents drying
• › prevents mold formation

Answer 3

Egg white

Glycerol

Thymol crystals

Question 4

Adhesives

Outdated blood stored in blood banks, dispensed into sterile tubes of 0.5 ml each.

Not ideal because there might be the presence of pathogens.

Answer 4

Plasma

Question 5

Adhesives

Better adhesion than albumin;
Heated prior to using;
Added to floatation bath;
Stains with many dyes.

Answer 5

1% Gelatin

Question 6

Adhesives

• Does not retain the stain.

Answer 6

1% Methyl Cellulose

Question 7

Adhesives

• No background staining:
• Most commonly used IHC adhesive.

Answer 7

Poly-L Lysine

Question 8

Adhesives

Strong adhesive;
Slight dye retention, not affected by mild alkaline solutions 3 months.

Disadvantage: Blackening in silver impregnation staining, reticulin methods, red staining in methyl green pyronin technique.

Answer 8

Sodium silicate

Question 9

Adhesives

Diluted 1:10 with acetone;

Has the greatest adhesion;

One kind is araldite (made of epoxy resins).

Answer 9

Resins

Question 10

Sections are floated out on a water bath set at

Answer 10

45-50°C.

Question 11

The temperature of the water should be between____ below the melting point of the paraffin wax.

Answer 11

5 and 10°C

Question 12

may be added for reducing surface tension and allowing the section to flatten out with greater ease.

Answer 12

Alcohol or small quantities of detergent

Question 13

Drying

With a temperature setting at the melting point of the wax no obvious damage is done to the sections and drying is complete in…

Answer 13

30 minutes.

Question 14

True or False

A _____may also be used instead of a drying oven.

Answer 14

hot plate

Question 15

For more delicate tissues such as brain, a lower drying temperature is used to avoid splitting and cracking of the section due to excessive heat. In such cases,____ to ____ hours or longer is recommended.

Answer 15

37o C for 24

Question 16

Slide Dryer: _____above the melting point of paraffin

Answer 16

5-10°C

Question 17

• These tools are needed for handling sections during cutting, and for removing folds and creases on the sections during “floating out” in water bath.

Answer 17

Forceps (fine pointed or curved) and squirrel hair brush

Question 18

…slides are generally used, where the identification number of the section can be inscribed with a pencil.

Answer 18

Frost-ended

Question 19

Equipment such as a____ is made on the assumption that regular slides have been used

Answer 19

slide rack

Question 20

The quality of sections cut on a microtome suffer badly from several (avoidable) causes. Things to avoid include

Answer 20

Fecal material
Hair