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Histopath LEC > H&E Staining > Flashcards

H&E Staining Flashcards

1
Q

1-3

A

Xylene 3mins

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2
Q

4,5,6

A

100% ETOH - 2mins and 30secs

100% ETOH - 2mins

95% ETOH - 2mins and 30secs

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3
Q

7

A

Running tap water - 3mins

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4
Q

8

A

Hematoxylin - 5mins

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5
Q

9

A

Running tap water - 2mins

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6
Q

10

A

Acid alcohol - 1min and 30secs

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7
Q

11

A

Bluing agent - 1min and 30secs

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8
Q

12

A

95% ETOH - 1min

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9
Q

13

A

Eosin - 4 mins

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10
Q

14,15,16

A

95% ETOH - 2mins

100% ETOH - 3mins

100% ETOH - 3mins

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11
Q

17,18

A

Xylene - 3mins

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12
Q

Purpose of first 3 xylene

A

Deparaffinization

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13
Q

What is not included during frozen section biopsy?

A

First 3 xylene

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14
Q

Purpose of decreasing concentration of ETOH?

A

Rehydration

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15
Q

Purpose of rehydration

A

To make hematoxylin penetrate better by opening the tissue pores

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16
Q

What happens if rehydration is not done appropriately

A

Hematoxylin will not be absorbed

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17
Q

Primary stain

A

Hematoxylin

18
Q

Purpose of acid alcohol

A

Differentiator or Decolorizer

Enhance the performance of BLUING agent and remove excess hematoxylin

19
Q

Enhance the performance of BLUING agent and remove excess hematoxylin

A

Acid alcohol

20
Q

Bluing agent can be:

A

Scott’s tap water
Ammonia water

21
Q

Scott’s tap water

A

Sodium bicarbonate NaHCO₃ + Magnesium sulfate MgSO4

22
Q

is a bluing solution used in histology to intensify the staining of cell nuclei and basophilic structures in tissue sections after hematoxylin staining

A

Scott’s solution

23
Q

Purpose of bluing agent

A

Enhances primary stain (hematoxylin)

24
Q

Enhances eosin stain

25
Q

Acidic stain
Counter stain
Secondary stain
Background stain

26
Q

Purpose of increasing concentrations of ETOH

A

Dehydration

27
Q

Purpose of dehydration

A

Remove water in preparation for mounting with xylene

28
Q

Purpose of last batch of xylene

A

Increase the refractive index

Important for easier mounting

29
Q

is the corner stone of tissue-based diagnosis

The process stains thin tissue sections so that pathologists can visualize tissue morphology

A

Hematoxylin and Eosin (H&E) staining

30
Q

The process uses a hematoxylin dye to stain cell nuclei (and other parts)_____ and an eosin dye to stain other structures____

A

blue

pink or red

31
Q

binds strongly to acids and consequently binds to nuclear DNA and stains nuclei blue.

Properly applied, this technique provides exceptional detail of tissue structure and the makeup of the cells.

This detail is required for tissue-based diagnosis, particularly in the detection and classification of infection, cancer or metabolic disease

A

Hematoxylin

32
Q

plays a significant role in tissue-based diagnosis by coloring otherwise transparent tissue sections, and allowing cell structures including the cytoplasm, nucleus, and organelles and extra-cellular components to be clearly visible under the microscope

A

Routine H&E staining

33
Q

In a histology laboratory, all specimens are initially stained with_____ and additional stains are only ordered if additional information is needed to provide a more detailed analysis.

34
Q

The term______ is used by pathologists to indicate any area of damage, infection, inflammation, tumor, necrosis or otherwise abnormal tissue.

35
Q

ROUTINE H&E STAINING in Paraffin Embedded Section

Progressive or Regressive?

A

Regressive Staining

36
Q

Routine H&E staining

Fixation: Most fixatives can be used except_____ solutions which inhibit hematoxylin.

A

osmic acid

37
Q

Differentiate in_______ (________)

A

1% acid-alcohol

1 ml concentrated HCl to 99 ml of 80% ethyl alcohol

38
Q

Bluing agent

Blue in_______ (average of 5 minutes) or ________until the sections appear blue (about 30 seconds).

A

ammonia water

1% aqueous lithium carbonate

39
Q

Counterstain with ______for 5 minutes. If alcoholic eosin is used, the time can be reduced to 30 seconds or 1 minute

A

5% aqueous eosin

40
Q

For tissues fixed with_______, the staining time in hematoxylin should be increased slightly while duration of eosin staining should be reduced

A

mercuric chloride

41
Q

The following staining methods are commonly employed for frozen sections, the choice depending upon the personal preference of the pathologist and the type of tissue section to be stained.

A
  1. Hematoxylin-Eosin method
  2. Thionine method
  3. Polychrome Methylene Blue method
  4. Alcoholic Pinacyanol method
42
Q

H & E staining of Frozen Sections for Rapid Diagnosis

Progressive or Regressive?

A

Progressive Staining

Histopath LEC (37 decks)

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