Other Stains

Question 1

• Basic nuclear stain; diagnosis of
Diphtheria; Fresh sputum; Aerogenes bac

Answer 1

METHYLENE BLUE

Question 2

• MB is heated with fixed alkali/alkali carbonate

Answer 2

METHYL VIOLET

Question 3

Sub for Thionine in FFT
• For Nissl granules

Answer 3

TOLUIDINE BLUE

Question 4

• Amyloid in FFT; Platelets
• For Hematology

Answer 4

CRYSTAL VIOLET

Question 5

• Counterstaining of epithelial sections

Answer 5

ANILINE BLUE

Question 6

• Plasma stain for Acid-fast organism

Answer 6

BASIC FUCHSIN

Question 7

Ascaris eggs and erythrocyte; Bacterial spore stain
Both decolorizer and counterstain
• Usually for Parasitology

Answer 7

MALACHITE GREEN

Question 8

• Contrast stain for Gran’s staining

Answer 8

BISMARCK BROWN

Question 9

• Utilized for manufacture of paints

Answer 9

PRUSSIAN BLUE

Question 10

• Acid mucopolysaccharide

Answer 10

ALCIAN BLUE

Question 11

o For Embryos

Answer 11

CONGO RED

Question 12

• For Mitochondria

Answer 12

JANUS GREEN

Question 13

• Substitute for Carbole Fuchsin

Answer 13

NIGHT BLUE

Question 14

is used to identify deposits of protein in tissue called amyloid.

Answer 14

Congo red

Question 15

• is commonly used in histology to stain nervous tissues.

stains the acidic components of the neuronal cytoplasm (specifically Nissl bodies) a violet color.

Answer 15

CRESYL VIOLET

Question 16

intercalates and stains DNA, providing a fluorescent red-orange stain.

Although it will not stain healthy cells, it can be used to identify cells that are in the final stages of apoptosis –such cells have much more permeable membranes

Answer 16

ETHIDIUM BROMIDE

Question 17

is often used as a marker for apoptosis in cells populations and to locate bands of DNA in gel electrophoresis.

Answer 17

ethidium bromide

Question 18

– consists of a mixture of methylene-blue and eosin, and it is used for staining blood to differentiate leukocytes.

Answer 18

GIEMSA STAIN

Question 19

• is the stain used for metallic impregnation, made up of gold chloride and mercuric chloride

Answer 19

GOLD SUBLIMATE

Question 20

• is probably the oldest of all stains, originally used for microscopic study of starch granules

Answer 20

IODINE

Question 21

• Used to stain lipids or fats
• “Sudan” is specific for lipids

Answer 21

LYSOCHROMES

Question 22

LYSOCHROMES

Answer 22

Sudan black
Sudan IV
Sudan lll

Question 23

• For Phospholipids & Neutral Lipids
• 0.5% solution boiled in 70% ethanol

Answer 23

SUDAN BLACK

Question 24

• For neutral lipids only
• 2% Benzoic acid in 70% Alcohol

Answer 24

SUDAN IV

Question 25

Fat stain for CNS

Answer 25

SUDAN Ill

Question 26

CHIEF SOLVENTS USED FOR STAINS

Answer 26

Water
Alcohol
Aniline water
Phenol

Question 27

1. • should always be distilled unless otherwise stated.
2. • Ethyl alcohol may be used in various concentrations. Methyl alcohol, if to be used, is usually absolute, and is indicated especially in the preparation of blood stains, for which reason, it should be acetone free.
3. -Ten ml. of aniline is added to every 1/2 to 1 liter of hot distilled water, shaken, cooled, and filtered.
4. • is used in aqueous solution of 0.5 - 5%.

Answer 27

WATER

ALCOHOL

ANILINE WATER

PHENOL

Question 28

OTHER MATERIALS USED IN STAINING

Answer 28

COPLINIAR

SLOTTED STAINING DISHES

METAL/GLASS STAINING
RACK/CARRIES

Question 29

OTHER MATERIALS USED IN STAINING:

COPLINIAR

SLOTTED STAINING DISHES

METAL/GLASS STAINING
RACK/CARRIES

Answer 29

Holes 5-9 slides

Holds 5-19 slides

Holds 10-30 slides