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Histopath LEC > STAINS_COLOR > Flashcards

STAINS_COLOR Flashcards

1
Q

PAS

A

Magenta red

CHO, Glycogen, Mucins, Bacteria & Fungi, basement membrane

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2
Q

PAS w/ diastase

A

Red

Glycogen

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3
Q

Best carmine

A

Bright red

Glycogen

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4
Q

Langhan’s iodine

A

Mahogany brown

Glycogen

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5
Q

Alcian blue

A

Blue

Acid mucin

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6
Q

Alcian blue-PAS

A

Acid mucin: blue
Neutral mucin: magenta

Any mucins (acid/neutral)

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7
Q

Gomori’s aldehyde fuchsin stain

Sulfated mucins:
Carboxylated mucins:

A

purple
blue

Acid MPS Sulfated mucins Carboxylated mucins

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8
Q

Mucicarmine stain

A

Red

Cryptococcus neoformans Mucins

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9
Q

Colloidal iron

A

Dark blue

Acid mucin

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10
Q

Acridine orange

Acid mps
Fungi

A

Black
Greenish red fluorescence

Acid mucins/MPS Fungi

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11
Q

Sudan black

A

Blue black

Lipids

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12
Q

Sudan IV

A

Red

Lipids (TAG)

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13
Q

Sudan lll

A

Red

CNS

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14
Q

Oil red O

A

Brilliant red

Lipids

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15
Q

Osmium tetroxide

A

Black

Lipids

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16
Q

Nile blue sulfate method

Neutral fat
Cholesterin esters
Cholesterin fatty acids
Fatty acids & soap
Cerebrosides

A

= Pinkish red
= Light red
= Light red
= Deep blue to violet
= Light blue

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17
Q

Toluidine blue-acetone mtd

A

Metachromatic redbrown or yellow

Sulfatide

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18
Q

Borohydride-Periodic-Schiff method

A

Red

Gangliosides

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19
Q

Alkaline fast-green method

A

Green

Histones
Protamines

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20
Q

Peracetic acid-Alcian blue

A

Blue-green

Cystine
Cysteine

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21
Q

Sakaguchi

A

Orange-r

Arginine

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22
Q

Gomori calcium

A

Brownish black

Alkaline phosphatase

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23
Q

Gomori lead method

A

Black

Acid phosphatase

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24
Q

Lead method

A

Blackish brown

5’-nucleotidase

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25
Q

Metal precipitation

A

Dark brownish-black ppt

ATPase

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26
Q

Calcium cobalt method

A

Cobalt phosphate ppt

ATPase

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27
Q

α-naphthyl acetate method

A

Reddish brown

Non specific esterase

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28
Q

Indoxyl acetate method

A

Blue

Nonspecific esterase

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29
Q

Tetrazolium method

A

Bluish black

Monoamine oxidase

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30
Q

Feulgen technique

A

Red-purple

DNA

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31
Q

Methyl green-pyronin

A

RNA (nucleoli): red
DNA (chromatin): green

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32
Q

Gomori’s silver impregnation stain

A

Black

Reticulin fibers

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33
Q

Van Gieson’s stain

A

Collagen = Pink/deep red

Muscle, cytoplasm, RBC, fibrin = Yellow

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34
Q

Masson’s trichrome stain

A

Collagen & mucus = Blue

Muscle, RBC & keratin = Red

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35
Q

Mallory’s aniline blue

A

Collagen fibers, cytoplasm, fibroglia fibrils, axon cylinders, neuroglia = Red

Elastic fibers = Pale pink/yellow

RBCs, myelin sheets = Yellow

36
Q

Azocarmine

A

CT Glomerular basement membrane Amyloid & mucous colloid

= Deep blue

37
Q

Weigert’s

A

Elastic fibers

Dark-blue/blue-black

38
Q

Verhoeff’s

A

Elastic fibers

Black

39
Q

Taenzer-Unna-Orcein mtd

A

Elastic fibers

Dark-brown

40
Q

Krajian’s technique

A

Elastic fibers = Bright red
Fibrin & CT = Dark blue
RBC = Orange-yellow

41
Q

Martius-Scarlet-Blue

A

RBCs = Yellow

Muscle = Red

Collagen = Blue

Fibrin = Red

42
Q

Mallory’s PTAH

A

Fibrin, muscle striations, neuroglia, amoeba = Dark blue

RBCs = Blue

Myelin = Lighter blue

Collagen, osteoid, cartilage, elastic fibers = Deep brownish-red

43
Q

Congo red

A

Amyloid

Red

44
Q

Methyl violet-crystal violet method

A

Amyloid

Purplish red

45
Q

Thioflavin-T fluorescent staining

A

Amyloid

Yellow fluorescence

46
Q

Modified Gomori’s Trichrome stain

A

Muscle fibers = Red

Collagen = Green

47
Q

Lissamine fast red

A

Muscles, RBC = Red
Collagen = Yellow

48
Q

Schmorl’s Picro-Thionin method

A

Lacunae & canaliculi = Dark brown-black

Bone matrix = Yellow/brownish-yellow

49
Q

Bielschowsky’s technique

A

Neurofibril, axons & dendrites = Black on a grayish BG

Neuroglia & collagen = Lightly stained

50
Q

Bodian’s stain

A

Nerve fibers & nerve endings

51
Q

Sevier-Munger technique

A

Peripheral neuritis = Black
Axons = Black
Myelin sheath = Light brown
Neuritic plaques & tangles = Black
Argentaffin granules = Black

52
Q

Toluidine blue

A

Nissl granules & nucleoli

Deep blue

53
Q

Polychrome methylene blue

A

Nissl granules & nucleoli

Deep blue

54
Q

Thionine

A

Nissl granuls & nucleoli

Purple

55
Q

Cresyl fast violet

A

Nissl substance = Purple-dark blue
Neurons = Pale purple blue

56
Q

Weigert-Pal technique

A

Myelin sheath

Blue black

57
Q

Luxol fast blue

A

Myelin

Blue-green

58
Q

Weil’s method

A

Myelin

Black

59
Q

Cajal’s gold sublimate method

A

Astrocytes

Black on a light brownish BG

60
Q

Perl’s Prussian blue

A

Hemosiderin

Deep blue

61
Q

Gomori’s Prussian blue

A

Iron pigments

Bright blue

62
Q

Turnbull’s blue

A

Ferrous iron (Hemosiderin)

Blue

63
Q

Benzidine-nitroprusside stain

A

Hemoglobin & oxidase granules

Dark blue

64
Q

Mod. Fouchet’s technique

A

Bile pigments

Emerald to blue green

65
Q

Gmelin technique

A

Bile & hematoidin

Blue-purple then green

66
Q

Stein’s iodine test

A

Bile pigments

67
Q

Schmorl’s ferric ferricyanide method

A

Bile, lipofuscins, melanin, argentaffin cells, chromaffin, thyroid colloid

Dark blue

68
Q

Gomori’s aldehyde fuchsin

A

Lipofuscin

Purple

69
Q

Hemofuscin

Red

A

Mallory’s fuchsin stain

70
Q

Masson Fontana technique

A

Melanin = Black
Argentaffin cell granules = Black

71
Q

Von Kossa’s silver nitrate method

A

Calcium

Black

72
Q

Lindquist modified rhodanine technique

A

Copper

Red to orange-red

73
Q

Gram-Twort stain

A

Gram (+) organisms = Blue-black
Gram (-) organisms = Pink-red
RBCs = Green
Elastic fibers = Black

74
Q

Brown & Brenn method

A

Gram (+) bacteria = Blue
Gram (-) bacteria = Red

75
Q

Wade-Fite technique

A

M. leprae = Golden yellow

76
Q

Toluidine blue

A

H. pylori = Dark blue against blue BG

77
Q

Cresyl violet acetate mtd

A

H. pylori = Blue-violet

78
Q

Dieterle method

A

L. pneumophila & spirochetes = Dark brown to black

79
Q

Levaditi’s method

A

Spirochetes = Black on a yellowish BG

80
Q

Modified Steiner & Steiner technique

A

Spirochetes, Donovan bodies, fungi, bacteria = Black

81
Q

Warthin-Starry method

A

Spirochetes = Black

82
Q

Grocott Methenamine Silver

A

Fungi = Sharply outlined I black
Mucin & glycogen = Gray-black
Mycelia & hyphae = Old rose
RBCs = Yellow

83
Q

Lendrum’s phloxinetartrazine method

A

Viral inclusions = Bright red

84
Q

Orcein method

A

HBsAg = Brown-black

85
Q

Giemsa stain

A

Bacteria = Blue
Mast cell granules = Deep blue
Eosinophilic granules = Red
Nuclei = Blue
Cytoplasm = Pink

Histopath LEC (37 decks)

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