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Histopath LEC > STAINS_Comments > Flashcards

STAINS_Comments Flashcards

1
Q

: essential component of Schiff reagent

A

Basic fuchsin

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2
Q

Method of choice for glycogen staining

A

PAS w/ diastase ctrl

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3
Q

Selective & highly specific for glycogen

A

Nest carmine

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4
Q

Obsolete Not specific for glycogen

A

Langhan’s iodine

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5
Q

Avoid celloidinization of slides

A

Alcian blue

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6
Q

Avoid Ehrlich’s hematoxylin

A

Alcian blue PAS

Mucicarmine stain

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7
Q

Acridine orange Lasts for only

A

2 hrs

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8
Q

Most commonly used stain

A

Sudan IV (Scharlach R)

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9
Q

: preliminary indicator of the type of lipid present

A

Nile blue

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10
Q

Nile blue sulfate

(dissol. neutral lipids)

(reacts w/ PL and FFA)

A

-Red oxazone

-Blue oxazone

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11
Q

stains basic groups in tissues

A

Alkaline Fast green

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12
Q

Sakaguchi Uses_____ reagent

A

Milton

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13
Q

Substrate: β-glyceroPO4

A

Gomori lead

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14
Q

Substrate: β-glyceroPO4

A

Gomori lead

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15
Q

Substrate: β-glyceroPO4

A

Gomori lead

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16
Q

For skel. muscle biopsies

A

Metal ppt
Calcium cobalt method

17
Q

Most reliable & specific histochemical staining technique for DNA

Contains Schiff’s reagent

A

Feulgen technique

18
Q

Reticulin = Argyrophilic (silver stain)

A

Gomori’s silver impregnation stain

19
Q

Van Gieson’s stain

Contains

A

acid fuchsin & picric acid

20
Q

(-) Fuchsin: Excellent & colorful method of demonstrating CT fibers

A

Mallory’s aniline blue

21
Q

Heidenhain’s modification of Mallory’s aniline blue stain

A

Azocarmine

22
Q

Martius-scarlet-blue

Early fibrin =
Old fibrin =

A

yellow

blue

23
Q

Diagnosis of Alzheimer’s disease

A

Bodian’s stain

24
Q

Nissl granules: a.k.a. Tigroid substances

A

Cresyl fast violet

25
Q

Depend on the oxidation of the pigment to green biliverdin by iodine

A

Stein’s iodine test

26
Q

: melanin reduces ammoniacal silver solutions w/o use of a reducer

A

Argentaffin reaction

27
Q

Recommended for blood and BM parasites, inclusion conjunctivitis, Toxoplasma, spirochetes & other bacteria

A

Giemsa stain

Histopath LEC (37 decks)

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