MOUNTING OF SECTIONS Flashcards
• To protect the specimen form physical injury
MOUNTING OF SECTIONS
MOUNTING OF SECTIONS
Recommended storage time is
10yrs
RE-STAINING OF OLD SECTIONS
Old, bleached or faded sections may be re-stained: the slide is usually immersed in_____ for_____, or gently_____ until the mounting medium begins to bubble.
The coverslip may then be removed by lifting it with a dissecting needle. The section is placed in____ for up to_____ to remove the remaining balsam and then brought down to water.
It is placed in a_____ solution for______, rinsed in tap water and subsequently immersed in_____ for_____ or until the section is decolorized.
After washing it again in _____water for another____, the section may then be re-stained with the appropriate staining technique
xylene; 24 hours
heated
xylene; 24 hours
0.5 potassium permanganate; 5-10 minutes
5% oxalic acid; 5 minutes
running tap; 5 minutes,
Mounting
• To protect the section from bleaching or deterioration due to____
• To_____ the slides for permanent keeping
• To facilitate easy handling and storage
• To prevent ____during microscopic examination
• Tissue should be impregnated with____ with an Index of Refraction close to that of the glass and tissue
• Few drops of mounting medium is placed on the section
oxidation
preserve
damage of sections
transparent medium
Characteristics of Good Mounting Medium
• Refraction Index near of glass (____)
1.518
Characteristics of Good Mounting Medium
• Refraction Index near of glass (1.518)
• Should not…
• Should not… out tissue sections
Should not cause…
• Should be…
dry quickly
dissolve
shrinkage and distortion
permanent
MOUNTING MEDIA GROUPS
Aqueous media
Resinous media
• Water-miscible preparations
AQUEOUS MEDIA
Water-low refractive index, moderately transparents, evaporated easily
AQUEOUS MEDIA
•_______ used when dehydration and clearing with Xylene cannot be made
•_______- Farrant’s Medium
•_______
Glycerin Gelly (RI: 1.47)
Gum Arabic (RI: 1.43)
Apathy’s Medium (RI: 1.52)
o Preparations that have been dehydrated and cleared in Xylene and Toluene
RESINOUS MEDIA
Resinous media
• Canada Balsam (1.524 RI) -
• Canadian Tree, Abus Balsamea
• DPX - 1.532 RI
• Xam - 1.520 RI
• Permount - 1.518 RI
• Clarite - 1.544 RI
DIFFICULTIES ENCOUNTERED DURING MOUNTING
• Not properly dehydrated =_____
•_____
• Setting may be hastened in a hot oven at_____ for ____
Cloudy or Milky with Xylene
Small bubbles
50°C for 2 hours
• Process of sealing the margins of the coverslip to prevent escape of fluid
RINGING
- 2 parts of paraffin wax mixed with
4-9 parts powdered Colophonium Resin (heated and filtered)
Kronig Cement
