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tagumpay Sa MARCH 2024! > SPECIMEN COLLECTION, HANDLING AND TRANSPORTTRANSPORT /AST > Flashcards

SPECIMEN COLLECTION, HANDLING AND TRANSPORTTRANSPORT /AST Flashcards

1
Q

Collection media for stool pathogens

A. Cary-blair
B. JEMBEC
C. LIM BROTH

A

A. Cary-blair

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2
Q

Commercial transport system used for N.gonorrhea specimens

A. Stuarts or Amie’s Cary-blair
B. JEMBEC system
C. LIM BROTH

A

B. JEMBEC system

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3
Q

Collection medium for vaginal swab (in pregnant)

A. Stuarts or Amie’s Cary-blair
B. JEMBEC
C. LIM BROTH

A

C. LIM BROTH

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4
Q

collection medium for stool

A

CLEAN LEAK-PROOF CONTAINER

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5
Q

Collection medium For corneal scrapings

A

Bedside plating (agar plate)

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6
Q

Collection medium for HAIR, NAILS, SKIN SCRAPINGS

A

Clean screw top tube

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7
Q

Specimen should be transported to the laboratory
________ of collection at room temp

A. 1 hour
B. 2 hours
C. 3 Hours
D. 30 mins

A

B. 2 hours

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8
Q

What specimen should be transported immediately to the laboratory

A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies

B. Unpreserved stool, gastric biopsy

C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings

D. Prostatic fluid in glass tubes, suprapubic aspirate, bone

A

D. Prostatic fluid in glass tubes, suprapubic aspirate, bone

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9
Q

In less than 15 mins.

A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies

B. Unpreserved stool, gastric biopsy

C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings

D. Prostatic fluid in glass tubes, suprapubic aspirate, bone

A

A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies

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10
Q

Within 1 hr.

A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies

B. Unpreserved stool, gastric biopsy

C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings

D. Prostatic fluid in glass tubes, suprapubic aspirate, bone

A

B. Unpreserved stool, gastric biopsy

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11
Q

Within 24 hours

A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies

B. Unpreserved stool, gastric biopsy

C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings

D. Prostatic fluid in glass tubes, suprapubic aspirate, bone

A

C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings

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12
Q

Added to media to absorb fatty acids present in specimen that could kill fastidious fragile organism (example N.gonorrhea or B.pertussis)

A

Charcoal

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13
Q

Bacteria in blood

A

BACTEREMIA

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14
Q

Bacteria increasing in numbers in the blood causing harm to the patient

A

SEPTICEMIA

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15
Q

Most common cause of sepsis

A

S. aureus

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16
Q

CSF (vial or bottle):
Submit bottle 1 to what section in the Laboratory and its temperature

17
Q

CSF (vial or bottle):
Submit bottle 2 to what section in the Laboratory and its temperature

A

Microbiology @ 37°C

18
Q

CSF (vial or bottle):
Submit bottle 3 to what section in the Laboratory and its temperature

A

Hematology Ref temp

19
Q

CSF (vial or bottle):
Submit bottle 4 to what section in the Laboratory

A

Microbiology

Leftover is for CC AND SERO

20
Q

CSF (vial or bottle) in CM sections should be kept at:

21
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

S.pneumoniae:

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

A. MHA with 5% sheep RBCs

22
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

MRSA

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C

23
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

H.influenzae

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)

Note: both HTM And MHA with sheep red blood cells are incubated in 5-7% CO2 for 18-20 hrs.

HTM- Haemophilus Test Medium

24
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

Anaerobes

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

25
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

Mycobacteria

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

F. Middlebrook 7H10

26
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

N.gonorrhoeae:

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Blood
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

E. GC agar with supplements

27
Q

AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:

N.meningitidis:

A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Blood
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours

A

D. MHA with 2-5% lysed Horse Blood

28
Q

Uses thin plastic strips impregnated on the undersurface with an antimicrobial concentration gradient and marked on the upper surface with a concentration index or scale:

A. D-test
B. E-test

29
Q

differentiate Clindamycin resistance among S.aureus resulting from efflux:

A. D-test
B. E-test

A

A. D-test

NOTE:
Clindamycin zone to give “D” pattern, indicating inducible clindamycin resistance

30
Q

AUTOMATED AST SYSTEMS:

64-well close card containing specified concentrations of antibiotics. The MIC results are validated with the Advanced Expert System or AES software.

A. Vitek legacy and Vitek 2 systems
B. WalkAway system
C. Phoenix system

A

A. Vitek legacy and Vitek 2 systems

31
Q

AUTOMATED AST SYSTEMS:

Uses the broth microdilution panel format that is manually inoculated with the multiprong device;

Bacterial growth may be detected SPECTROPHOTOMETRICALLY or FLUOROMETRICALLY

A. Vitek legacy and Vitek 2 systems
B. WalkAway system
C. Phoenix system

A

B. WalkAway system

32
Q

AUTOMATED AST SYSTEMS:

Manual, gravity-based Inoculation process:

A. Vitek legacy and Vitek 2 systems
B. WalkAway system
C. Phoenix system

A

C. Phoenix system

33
Q

Separates bacterial plates allowing for optimal temperatures separate racks.
Used in automated microbiology labs.

A

Smart incubator

34
Q

The highest concentration of the drug is found in the:

A

Area closest to the disk

35
Q

SUPPLEMENTAL METHODS FOR DETECTING ANTIMICROBIAL RESISTANCE:

Detection of staphylococcal resistance to penicillinase-resistant-penicillins

A. OXACILLIN DISK SCREEN
B. OXACILLIN AGAR SCREEN
C. VANCOMYCIN AGAR
D. AMINOGLYCOSIDE SCREEN

A

B. OXACILLIN AGAR SCREEN

36
Q

SUPPLEMENTAL METHODS FOR DETECTING ANTIMICROBIAL RESISTANCE:

Detection of enterococcal resistance to vancomycin

A. OXACILLIN DISK SCREEN
B. OXACILLIN AGAR SCREEN
C. VANCOMYCIN AGAR
D. AMINOGLYCOSIDE SCREEN

A

C. VANCOMYCIN AGAR

37
Q

SUPPLEMENTAL METHODS FOR DETECTING ANTIMICROBIAL RESISTANCE:

Detection of acquired in ENTEROCOCCAL HIGH-LEVEL RESISTANCE to aminoglycosides that would compromise synergy with a cell wall-active agent such as ampicillin or vancomycin

A. OXACILLIN DISK SCREEN
B. OXACILLIN AGAR SCREEN
C. VANCOMYCIN AGAR
D. AMINOGLYCOSIDE SCREEN

A

D. AMINOGLYCOSIDE SCREEN

38
Q

SUPPLEMENTAL METHODS FOR DETECTING ANTIMICROBIAL RESISTANCE:

Detection of Streptococcus pneumoniae resistance to penicillin

A. OXACILLIN DISK SCREEN
B. OXACILLIN AGAR SCREEN
C. VANCOMYCIN AGAR
D. AMINOGLYCOSIDE SCREEN

A

A. OXACILLIN DISK SCREEN

tagumpay Sa MARCH 2024! (19 decks)

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