SPECIMEN COLLECTION, HANDLING AND TRANSPORTTRANSPORT /AST Flashcards
Collection media for stool pathogens
A. Cary-blair
B. JEMBEC
C. LIM BROTH
A. Cary-blair
Commercial transport system used for N.gonorrhea specimens
A. Stuarts or Amie’s Cary-blair
B. JEMBEC system
C. LIM BROTH
B. JEMBEC system
Collection medium for vaginal swab (in pregnant)
A. Stuarts or Amie’s Cary-blair
B. JEMBEC
C. LIM BROTH
C. LIM BROTH
collection medium for stool
CLEAN LEAK-PROOF CONTAINER
Collection medium For corneal scrapings
Bedside plating (agar plate)
Collection medium for HAIR, NAILS, SKIN SCRAPINGS
Clean screw top tube
Specimen should be transported to the laboratory
________ of collection at room temp
A. 1 hour
B. 2 hours
C. 3 Hours
D. 30 mins
B. 2 hours
What specimen should be transported immediately to the laboratory
A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies
B. Unpreserved stool, gastric biopsy
C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings
D. Prostatic fluid in glass tubes, suprapubic aspirate, bone
D. Prostatic fluid in glass tubes, suprapubic aspirate, bone
In less than 15 mins.
A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies
B. Unpreserved stool, gastric biopsy
C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings
D. Prostatic fluid in glass tubes, suprapubic aspirate, bone
A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies
Within 1 hr.
A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies
B. Unpreserved stool, gastric biopsy
C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings
D. Prostatic fluid in glass tubes, suprapubic aspirate, bone
B. Unpreserved stool, gastric biopsy
Within 24 hours
A. Sterile body fluids, gastric aspirate, tissue biopsy, corneal scrapings, foreign bodies
B. Unpreserved stool, gastric biopsy
C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings
D. Prostatic fluid in glass tubes, suprapubic aspirate, bone
C. Preserve stool, rectal swab, preserved urine, hair, nail, skin scrapings
Added to media to absorb fatty acids present in specimen that could kill fastidious fragile organism (example N.gonorrhea or B.pertussis)
Charcoal
Bacteria in blood
BACTEREMIA
Bacteria increasing in numbers in the blood causing harm to the patient
SEPTICEMIA
Most common cause of sepsis
S. aureus
CSF (vial or bottle):
Submit bottle 1 to what section in the Laboratory and its temperature
CC frozen
CSF (vial or bottle):
Submit bottle 2 to what section in the Laboratory and its temperature
Microbiology @ 37°C
CSF (vial or bottle):
Submit bottle 3 to what section in the Laboratory and its temperature
Hematology Ref temp
CSF (vial or bottle):
Submit bottle 4 to what section in the Laboratory
Microbiology
Leftover is for CC AND SERO
CSF (vial or bottle) in CM sections should be kept at:
Room Temp
AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:
S.pneumoniae:
A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours
A. MHA with 5% sheep RBCs
AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:
MRSA
A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:
H.influenzae
A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
Note: both HTM And MHA with sheep red blood cells are incubated in 5-7% CO2 for 18-20 hrs.
HTM- Haemophilus Test Medium
AST: IN THE CASE OF FASTIDIOUS MICROORGANISMS:
Anaerobes
A. MHA with 5% sheep RBCs
B. HTM is used MHA supplemented with hematin, NAD, and Yeast extract)
C. MHA with 2% NaCl incubated for 24hrs at 30 to 35°C
D. MHA with 2-5% lysed Horse Bloof
E. GC agar with supplements
F. Middlebrook 7H10
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours
G. Brucella blood agar with Hemin incubated anaerobically up to 48 hours