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tagumpay Sa MARCH 2024! > Family ENTEROBACTERIACEAE > Flashcards

Family ENTEROBACTERIACEAE Flashcards

1
Q

All ENTEROBACTERIACEAE is CATALASE POSITIVE Except:

A

S. Dysenteriae

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2
Q

All ENTEROBACTERIACEAE are OXIDASE NEGATIVE Except:

A

Plesiomnas

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3
Q

Capsular antigen; heat labile

A. K antigen
B. O antigen
C. H antigen

A

A. K antigen

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4
Q

Somatic antigen; Heat stable;
Antibodies are predominanty IgM

A. K antigen
B. O antigen
C. H antigen

A

B. O antigen

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5
Q

Located in the flagella;
Dentatured or removed by heat or alcohol;
Mainly IgG

A. K antigen
B. O antigen
C. H antigen

A

C. H antigen

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6
Q

RAPID LACTOSE FERMENTERS:

A

“EKE”
Escherichia
Klebsiella
Enterobacter

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7
Q

Late lactose fermenter:

A

” Cit Ser, Yer Haf Sa Shi is late”
Cit-robacter
Ser-ratia
Yer-sinia enterocolitica
Haf-nia
Sa-lmonella arizonae
Shi-gella sonnei

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8
Q

Non-lactose fermenter

A

Salmonella
Shigella
Proteus
Providencia
Morganella
Edwardsiella
Erwinia (plant pathogen)

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9
Q

CAUSES UTI 90%, SEPSIS, DIARRHEAL DISEASE

TSI: A/A GAS+ H2S-
IMVIC: ++–
LDC: +
ODC: +
ADH: -
COLONIES: GREEN METALLIC SHEEN ON EMB

A

E. COLI

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10
Q

MOST COMMON CAUSE OF UTI IN HUMANS

A

UROPATHOGENIC E.COLI (UPEC)

  • AEROBACTIN: CHELATES IRON
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11
Q

Cholera-like toxin

MONTEZUMA’S revenge/ turista/
Travellers diarrhea characterized
by profuse watery stool

A

ETEC/ ENTEROTOXIGENIC E.COLI

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12
Q

Shigella-like infection;
Dysentery; usually in young children living in areas of poor sanitation

Stool with RBCS, NEUTROPHILS AND MUCUS

A

EIEC: ENTEROINVASIVE E.COLI

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13
Q

Infantile diarrhea;
Non-invasive, seen in newborns and infants
Watery diarrhea with mucus but no blood

A

EPEC: ENTEROPATHOGENIC E.COLI

(EPEC inpant)*😂

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14
Q

Shiga/ Shigella-like toxin;
Most frequently asso. With certain serotypes such as E.COLIO157:H7

A

EHEC: ENTEROHEMORRHAGIC E.COLI /
VTEC: VEROTOXIN E.COLI

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15
Q

E.COLI O157:H7 on SMAC:

A

Colorless

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16
Q

Most secere manifestation of EHEC

A

HUS

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17
Q

Watery Diarrhea

A

EAEC: ENTEROAGGREGATIVE E.COLI

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18
Q

All E.coli is MUG+ EXCEPT:

A

E.COLIO157:H7

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19
Q

TSI: A/A GAS+ H2S-
IMVIC: –++
LDC: +
ODC: -
ADH: -
Nonmotile
Urease: +
COLONIES: exhibit mucoid growth, large polysaccharide
Pink mucoid on MAC

A

Klebsiella spp.

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20
Q

Friedlander’s bacillus
Encapsulated and appears as mucoid colonies that tend to string

A. K pneumoniae
B. K.rhinoscleromatis
C. K. ozaenae

A

A. K pneumoniae

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21
Q

Prurulent sinus infection
A. K pneumoniae
B. K.rhinoscleromatis
C. K. ozaenae

A

C. K. ozaenae

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22
Q

Granuloma of the nose and oropharynx
A. K pneumoniae
B. K.rhinoscleromatis
C. K. ozaenae

A

B. K.rhinoscleromatis

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23
Q

Asso. With plasmid-mediated ESBL’s

A

Klebsiella

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24
Q

IMVIC: +-++
Carry heat labile cytotoxin, that has been isolated from patients who have developed a self-limiting ANTIBIOTIC ASSO. HEMORRHAGIC COLITIS

A

K.oxytoca

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25
Q

Infections are asso with CONTAMINATED medical devices, such as respirators;

MOTILE
Lactose fermenters that produce mucoid colonies
TSI: A/A GAS+ H2S-
IMVIC: –++

A

Enterobacter spp.

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26
Q

Increasingly isolated from community Acquired pyrogenic liver abscess

A

K1 capsular containing K.pneumoniae

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27
Q

Indole negative

A. K.pneumoniae
B. K.oxytoca

A

A. K.pneumoniae

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28
Q

Indole Positive

A. K.pneumoniae
B. K.oxytoca

A

B. K.oxytoca

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29
Q

LDC: +
ODC: -
ADH: -

A. P.shigelloides
B. E.cloaceae
C. K.oxytoca, .K.pneumoniae
D. E. aerogenes
F. P.agglomerans

A

C. K.oxytoca, .K.pneumoniae

30
Q

LDC: +
ODC: +
ADH: -

A. P.shigelloides
B. E.cloaceae
C. K.oxytoca, .K.pneumoniae
D. E. aerogenes
F. P.agglomerans

A

D. E. aerogenes

31
Q

LDC: -
ODC: +
ADH: +

A. P.shigelloides
B. E.cloaceae
C. K.oxytoca, .K.pneumoniae
D. E. aerogenes
F. P.agglomerans

A

B. E.cloaceae

32
Q

LDC: -
ODC: -
ADH: -

A. P.shigelloides
B. E.cloaceae
C. K.oxytoca, .K.pneumoniae
D. E. aerogenes
F. P.agglomerans

A

F. P.agglomerans

33
Q

LDC: +
ODC: +
ADH: +

A. P.shigelloides
B. E.cloaceae
C. K.oxytoca, .K.pneumoniae
D. E. aerogenes
F. P.agglomerans

A

A. P.shigelloides

34
Q

Gained notoriety with a nationwide outbreak of septicemia resulting from contaminated IV fluid.

A. P.shigelloides
B. E.cloaceae
C. K.oxytoca, .K.pneumoniae
D. E. aerogenes
F. P.agglomerans

A

F. P.agglomerans

  • Pantoea agglomerans HG XII, produce yellow pigment, primarily a plant pathogen
35
Q

Produces Pink to Red colonies due to prod. Of Prodigiosin after incubation at 25°C

ONPG, DNAse, gelatinase, lipase: +
MDR / resistant to many antimicrobials
IMVIC: –++
TSIA: K/A GAS+ H2S-

A

Serratia spp.

36
Q

Most clinically significant Serratia spp.
Infection of urinary and respiratory tract, bacterimic outbreaks, septic arthritis

A. S.odorifera
B. S. marcescens
C. S.plymuthica

A

B. S. marcescens

37
Q

Has a musty and pugent odor or a ROTTEN POTATO-LIKE odor

A. S.odorifera
B. S. marcescens
C. S.plymuthica

A

A. S.odorifera

38
Q

Osteomyelitis was found following a motorcycle accident

A. S.odorifera
B. S. marcescens
C. S.plymuthica

A

C. S.plymuthica

39
Q

Asso with renal stone formation

Are motile, Non-LF capable of deaminating phenylalanine;

CULTURE MEDIA: Swarming appearance
ODOR: CHOCOLATE CAKE/ BURNT CHOCOLATE

A

Proteus spp.

  • Renal stone is called staghorn / struvite stone
40
Q

OX-2, OX-19
ACQUIRED B PHENOMENON

A. P.vulgaris
B. P.mirabilis

A

A. P.vulgaris

*Also E. coli 086

41
Q

OX-k
ACQUIRED A PHENOMENON

A. P.vulgaris
B. P.mirabilis

A

B. P.mirabilis

42
Q

IMVIC: -+vv
TSIA: K/A, GAS+, H2S+
LIA R/A
Indole -

A. P.vulgaris
B. P.mirabilis

A

B. P.mirabilis

43
Q

IMVIC: ++-v
TSIA: K/A, GAS+/-, H2S+
LIA R/A
Indole +

A. P.vulgaris
B. P.mirabilis

A

A. P.vulgaris

44
Q

ONPG+
LDC -

A. CITROBACTER SPP
B. SALMONELLA SPP

A

A. CITROBACTER SPP

45
Q

Asso. with infections disease acquired in hospital setting, UTI, pneumonia, inta-abdominal abscesses

Asso with endocarditis in IV drug abusers

80% H2S+
50% NON-LF
70% hydrolyze urea
LDC: NEGATIVE

A. C.freundii
B. C.koseri
C. Salmonella

A

A. C.freundii

46
Q

Cause of nursery outbreaks of neonatal meningitis and brain abscess

A. C.freundii
B. C.koseri
C. Salmonella spp.

A

B. C.koseri

47
Q

Sources of infection: water, milk, DAIRY PRODUCTS, shell fish, DRIED OR FROZEN EGGS, meat and meat products and houshold pets

Cause enterics, systemic infection and enteric fever (typhoid fever)

Motile
LDC+
NLF
H2S+
IMVIC -+-+
TSIA: K/A H2S+

A. C.freundii
B. C.koseri
C. Salmonella spp

A

C. Salmonella spp.

48
Q

Associated with Typhoid fever
IMVIC: -+–
TSIA: K/A GAS- H2S+
Black colonies with metallic sheen on BSA/ Bismuth sulfite Agar

A. Salmonella serotype Typhi
B. Salmonella serotype Enteriditis

A

A. Salmonella serotype Typhi

49
Q

Associated with infections acquired from the ingestion of EGGS OR CHICKENS

A. Salmonella serotype Typhi
B. Salmonella serotype Enteriditis

A

B. Salmonella serotype Enteriditis

50
Q

tube dilation aggl’n test:

A

Widal’s test

51
Q

In Widal’s test, at least 2 serum specimens obtained at intervals of ____-____ days, are needed to prove a rise in antibidy titer

A. 5-8 days
B. 6-9 days
C. 7-10 days
D. 8-11 days

A

C. 7-10 days

52
Q

High or rising titer of O>1:160 suggest _____ infection is present

A. Past
B. Active

A

B. Active

53
Q

High titer of H>1:160 suggest _____ infection

A. Past
B. Active

A

A. Past

54
Q

IMVIC:-+–
Nonmotile
Lysine decarboxylase -
Citrate -
Malonate-
H2S -
Non- LF
Gram Neg Rods that grow well on MacConkey agar

A. Yersinia spp.
B. Shigella spp.
C. Pseudomonas spp.

A

B. Shigella spp.

55
Q

Group A:
Catalase -
ONPG -
MANNITOL -

A. S. boydii
B. S. dysentriae
C. S. flexneri
D. S. sonnei

A

B. S. dysentriae

56
Q

Group B
Catalase +
ONPG -
MANNITOL +

A. S. boydii
B. S. dysentriae
C. S. flexneri
D. S. sonnei

A

C. S. flexneri

57
Q

Group C:
Catalase +
ONPG -
MANNITOL +

A. S. boydii
B. S. dysentriae
C. S. flexneri
D. S. sonnei

A

A. S. boydii

58
Q

Group D
Catalase +
ONPG +, LATE LF
MANNITOL +

A. S. boydii
B. S. dysentriae
C. S. flexneri
D. S. sonnei

A

D. S. sonnei

59
Q

Gram Negative Rods exhibit an unusual BIPOLAR STAINING ( weison stain)
Catalase -
Oxidase -
Indole +
Non-LF
Facultative anaerobes capable of growth at temp. Ranging from 4°C to 43°C

A. Yersinia spp.
B. Shigella spp.
C. Pseudomonas spp.

A

A. Yersinia spp.

  • REMEMBER!
    “ BIPOLAR si BAY”
    B-urkholderia
    A-. actinomycetemcomitans
    Y- ersinia
60
Q

Most commonly transmitted by the BITE of an infected rat flea (Xenopsylla cheopsis) resulting in BUBONIC PLAGUE; PNEUMONIC PLAGUE

Bioterrorism, NOT included in ENTEROBACTERIACEAE termed as “BLACK DEATH”

broth culture: exhibit a STALACTITE PATTERN
Inclusion: BIPOLAR Stain
Stain: wayson stain ( SAFETY PIN APPEARANCE)

A. Y. pseudotuberculosis
B. Y. enterocolitica
C. Y. Pestis

A

C. Y. Pestis

61
Q

Found in GI Tract of Swine, Rodents, Dogs
Can cause acute mesenteric lymphadenitis, which may present as APPENDICITIS

ENTEROCOLITIS: FEVER DIARRHEA AND ABDOMINAL PAIN (appendicitis like)

Culture: CIN (BULLSEYE appearance)
UREASE POSITIVE!
MOTILE AT ROOM TEMP
NON-MOTILE AT 37°C

A. Y. pseudotuberculosis
B. Y. enterocolitica
C. Y. Pestis

A

B. Y. enterocolitica

62
Q

Found in variety of wild and domesticated animals including Rodents, Birds and Rabbits

Infection similar to Y.enterocolitica but less common

A. Y. pseudotuberculosis
B. Y. enterocolitica
C. Y. Pestis

A

A. Y. pseudotuberculosis

63
Q

Most common cause of death associated with BACTERIAL CONTAMINATION OF PACKED RBC

A. Y. pseudotuberculosis
B. Y. enterocolitica
C. Y. Pestis

A

B. Y. enterocolitica

*CIN AGAR / YERSINIA SELECTIVE AGAR

64
Q

Bull’s neck INFECTION:

A. Y.enterocolitica
B. Enterobacter
C. Borrelia burgdorferi
D. E.histolytica
E. C.diphteriae

A

E. C.diphteriae

65
Q

Bull’s eye RASH (Erythema chronicum migrans):

A. Y.enterocolitica
B. Enterobacter
C. Borrelia burgdorferi
D. E.histolytica
E. C.diphteriae

A

C. Borrelia burgdorferi

66
Q

Bull’s eye AMOEBA:

A. Y.enterocolitica
B. Enterobacter
C. Borrelia burgdorferi
D. E.histolytica
E. C.diphteriae

A

D. E.histolytica

67
Q

Bull’s eye COLONIES on CIN:

A. Y.enterocolitica
B. Enterobacter
C. Borrelia burgdorferi
D. E.histolytica
E. C.diphteriae

A

A. Y.enterocolitica
*Also Aeromonas

68
Q

FISH eye COLONIES on EMB:

A. Y.enterocolitica
B. Enterobacter
C. Borrelia burgdorferi
D. E.histolytica
E. C.diphteriae

A

B. Enterobacter
*Pink colonies w/ dark center

69
Q

READ AND REMEMBER!

” SPACEd” = H2S POSITIVE
S-ALMONELLA
P-ROTEUS
A-RIZONAE
C-ITROBACTER FREUNDII
Ed-wardsiella tarda

A
70
Q

TSIA: K/A GAS - H2S - “ SPPY”
S-higella
P-rovidencia
P-lesiomonas
Y- ersinia

TSIA: K/A GAS+ H2S- “MCKS”
M-organella
C-itrobacter
K-oseri
S-erratia

TSIA: K/A GAS+ H2S+ “PECS”
P.mirabilis
E. tarda
C. Freundii
Salmonella

TSIA : A/A GAS+ H2S - “EKE”
E.coli
K-lebsiella
E-nterobacter

TSIA: K/K GAS- H2S- “PA”
P-seudomonas
A-lcaligenes

A
71
Q

ENTEROBACTERIACEAE MEMORIZE THIS!

INDOLE + “ May PEKPEC Po”?
M-organella
P-roteus vulgaris
E.coli
K.oxytoca
P-rovidencia
E. tarda
C-itrobacter koseri
P-lesiomonas

VP+ “KEESH”
K-lebsiella
E-wingella
E-nterobacter
S-erratia
H-afnia

Citrate + “Pero Sa CHEEKS”
P-rovidencia
S-erratia
C-itrobacter
H-afnia
E-wingella
E-nterobacter
K-lebsiella
S-almonella enteritidis

A
72
Q

MEMORIZE THIS!

Rapid UREASE Producer: “PPM”
P-roteus
P-rovidencia rettgeri
M-organella

Other RAPID UREASE Producer: “TPUNCH”
T. mentagrophytes (fungi)
P-PM (grp.)
U-reaplasma
N-ocardia
C.neoformans (fungi)
H.pylori (peptic ulcer)

SLOW UREASE Producer “CKEYS”
C-itrobacter
K-lebsiella
E-nterobacter gergoviae
Y. enterocolitica
S-erratia

A

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