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tagumpay Sa MARCH 2024! > Family STREPTOCOCCACEAE > Flashcards

Family STREPTOCOCCACEAE Flashcards

1
Q

Gram positive cocci,occurring in pairs or in chains Non-motile, Non Spore Forming, CATALASE NEGATIVE, Facultative Anaerobes,
Capnophilic 5 to 10%

A

Streptococcaceae

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2
Q

S.pneumoniae, Viridans streptococcus

A. Alpha
B. Beta
C. Gamma

A

A. Alpha

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3
Q

Group A (large zone), Group B (small zone), Group C Streptococcus

A. Alpha
B. Beta
C. Gamma

A

B. Beta

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4
Q

Group D (Non-Hemolytic)
A. Alpha
B. Beta
C. Gamma

A

C. Gamma

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5
Q

S. agalactiae

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

B. Group B strep

Note:
Beta hemolysis

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6
Q

S.dysagalactiae, S.equi

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

C. Group C strep

Note:
Beta hemolysis

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7
Q

S.pyogenes

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

A. Group A strep.

Note:
Beta hemolysis

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8
Q

Anginosus, mutans, mitis, salivarius

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

G. Viridans streptococcus

Note:
Mostly Alpha, Beta, Gamma hemolysis

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9
Q

S.bovis group

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

D. Group D Non Enterococcus

Note:
Alpha, Gamma hemolysis

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10
Q

E.faecalis, E.faecium

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

E. Group D Enterococcus

Note:
Alpha, Beta, and Mostly Gamma hemolysis

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11
Q

S.pneumoniae

A. Group A strep.
B. Group B strep
C. Group C strep
D. Group D Non Enterococcus
E. Group D Enterococcus
F. Pneumococcus
G. Viridans streptococcus

A

F. Pneumococcus

Note: alpha hemolysis

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12
Q

VIRULENCE FACTOR OF GROUP A S.pyogenes

Antiphagocytic for ADHERENCE to mucosal cells

A

M protein

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13
Q

Able to bind beta globulin Factor H, also binds to Fibrinogen Blocking Complement Alternate Pathway activation:

A

M PROTEIN

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14
Q

A serine protease capable of inactivating the Chemotactic Factor for Neutrophils And Monocytes:

A

C5a Peptidase (anapylatoxin)

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15
Q

Mediates ADHERENCE to host epithelial cells:

A

Protein F (fibronectin-binding protein)

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16
Q

Prevents opsonized phagocytosis, Mask Bacterial Antigens

A

Hyaluronic acid capsule

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17
Q

Degrades host DNA (DNase) and RNA

A

Streptodornase or DNases

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18
Q

Causes lysis of fibrin clots

A

Streptokinase (Fibrinolysin).

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19
Q

Solubilizes hyaluronic acid in connective tissue or Duran Reynal

A

Hyaluronidase

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20
Q

Subsurface hemolysin (Oxygen Labile)
Toxic to RBC’s WBCs and platelets
Induces antibody response

A. Streptolysin S
B. Streptolysin O

A

B. Streptolysin O

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21
Q

Surface hemolysin (oxygen stable)
Lysis WBCs and is non immunogenic

A. Streptolysin S
B. Streptolysin O

A

A. Streptolysin S

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22
Q

Associated with Scarlet Fever and Streptococcal Toxic Shock-like syndrome:

A. Exotoxin A
B. Endotoxin B

A

A. Exotoxin A

23
Q

Acute Spreading Erythematous lesion

A. Impetigo
B. Erysipilas
C. Cellulitis
D. Scarlet fever

A

B.Erysipilas

24
Q

Diffuse red rashes from chest to extremities

A. Impetigo
B. Erysipilas
C. Cellulitis
D. Scarlet fever

A

D. Scarlet fever

25
Q

Superficial and localized

A. Impetigo
B. Erysipilas
C. Cellulitis
D. Scarlet fever

A

A. Impetigo

26
Q

Involves deeper tissue invasion

A. Impetigo
B. Erysipilas
C. Cellulitis
D. Scarlet fever

A

C. Cellulitis

27
Q

ASO:
Elevated (children)
A.<166 todd units
B. >240 todd units
C. >320 todd units

A

C. >320 todd units

28
Q

ASO:
Normal
A.<166 todd units
B. >240 todd units
C. >320 todd units

A

A.<166 todd units

29
Q

ASO Moderately Elevated in Adults:
A.<166 todd units
B. >240 todd units
C. >320 todd units

A

B. >240 todd units

30
Q

Test for SCARLET FEVER:

+ Redness/erythema
Susceptibility to Scarlet fever

A. Dick’s test
B. Schultz-Charlton test

A

A. Dick’s test

31
Q

Test for SCARLET FEVER:

Neutralization test, Diagnostic test
+ Rash fades/”Blanching phenomenon”

A. Dick’s test
B. Schultz-Charlton test

A

B. Schultz-Charlton test

32
Q

Most common cause of neonatal meningitis:

A

Group B S. agalactiae

NOTE:
1. Transmission occurs vertically or during delivery
2. Causes Pneumonia and Neonatal Meningitis
3. In adult, causes Endometritis and wound infections

33
Q

Large-colony forming Beta hemolytic isolates
Resembles S. pyogenes infections

A. Group C Strep (S. dysagalactiae subsp. equisimilis)
B. S. anginosus group

A

A. Group C Strep (S. dysagalactiae subsp. equisimilis)

34
Q

Small colony forming beta hemolytic isolates resembles S.pyogenes infection

A. Group C Strep (S. dysagalactiae subsp. equisimilis)
B. S. anginosus group

A

B. S. anginosus group

35
Q

VIRULENCE FACTOR:
-Extracellular surface protein, serine protease and gelatinase for adhesion
-Cytolysins and resistance to antimicrobial agent
(MDR- MULTI DRUG RESISTANT) = HOSPITAL ACQUIRED OR NOSOCOMIAL INFECTION

ASSOCIATED DISEASE:
Nosocomial Infection, UTI (catheterization) Bacteremia (hemodialysis, surgery)
Endocarditis (Prosthetic Heart Valves)

A

Group D Enterococcus

36
Q

VIRULENCE FACTOR: (Oral Flora URT)
-Enhance attachment to host cell surfaces
-Polysaccharide capsule, Extracellular Dextran and cell surface associated proteins (adhesins)

ASSOCIATED DISEASE:
-Subacute bacterial endocarditis from patients with damage heart valves
-Bacteremia, Septicemia and Cavities
-Dental Infection

A

Viridians streptococci Group A, C, F, G, N)

NOTE:
CAUSATIVE ORAL FLORA:
GRAM POSITIVE=VIRIDIANS
GRAM NEGATIVE= HACEK

37
Q

Common cause of bacterial meningitis in adults

VIRULENCE FACTOR:
✓Capsular polysaccharide
✓Antigenic polysaccharide resulting to 80 serogroups
✓Antiphagocytic

A

Streptococcus pneumoniae
>29 yrs old age group

38
Q

1 cause of “MOPS”

A

Streptococcus pneumoniae

Note: MOPS
MENINGITIS
OTITIS MEDIA
PNEUMONIA
SINUSITIS

39
Q

MORPHOLOGY AND OTHER CHARACTERISTICS:
✓Gram positive diplococci (lancet, flame or bullet shape)
✓ Growth on BAP : WHITE “PINPOINT”
✓CATALASE NEGATIVE
✓Capnophilic
✓neufeld reaction (Quellung, capsular swelling) POSITIVE

A

Streptococcus pneumoniae

Note:
Neufeld reaction is replaced by MOLECULAR TECHNIQUES

40
Q

Sputum:

Rust colored sputum

A. S.pneumoniae
B. K.pneumoniae
C. P.aeruginosa

A

A. S.pneumoniae

41
Q

Sputum:
Currant jelly

A. S.pneumoniae
B. K.pneumoniae
C. P.aeruginosa

A

B. K.pneumoniae

42
Q

Sputum:
Blue-green

A. S.pneumoniae
B. K.pneumoniae
C. P.aeruginosa

A

C. P.aeruginosa

43
Q

Growth in 6.5% NaCl +
PYR+
Bile Esculin hydrolysis +

A. D Enterococcus
B. D non-Enterococcus

A

A. D Enterococcus

Note:
+BILE ESCULIN: Brown-Black precipitate

44
Q

Growth in 6.5% NaCl -
PYR-
Bile Esculin hydrolysis+
A. D Enterococcus
B. D non-Enterococcus

A

B. D non-Enterococcus

Note:
+BILE ESCULIN: Brown-Black precipitate

45
Q

Skin test for Pneumococcal antibodies

A

FRANCIS test

46
Q

PYRase test POSITIVE:

A. Group A strep
B. Group B strep
C. Group C strep

A

A. Group A Strep (S. pyogenes)

47
Q

CAMP test :
Enhanced hemolysis, Arrowhead Pattern:

A. Group A strep
B. Group B strep
C. Group C strep

A

B. Group B strep (S. agalactiae)

48
Q

CAMP test :
Rectangular or Block Shape or Pattern:

A. S.aureus
B. Listeria
C. S. agalactiae

A

B. Listeria

49
Q

Hippurate hydrolysis +
Deep blue /purple color

A. S.pyogenes
B. S.agalactiae

A

B. S.agalactiae (Group B strep)

50
Q

Reagent: PDAC
+ BLUE

A. Spot Indole test
B. Indole test

A

A. Spot Indole test

51
Q

PDAB
+CHERRY RED

A. Spot Indole test
B. Indole test

A

B. Indole test

52
Q

Test for: pyrrolidinyl aylamidase
Substrate: L-pyrrolidonyl-B-naphthylamide
Color Developer: PDAC + CHERRY RED
Positive result: RED
POSITIVE FOR GROUP A AND ENTEROCOCCI

A

PYRase

53
Q

✓Alpha hemolytic
✓Bile solubility + (soluble)
✓ Optochin Sensitive( >14mm)
✓Neufeld Quellung: Positive (+) capsular swelling

A. S. pneumoniae
B. Viridians

A

A. S. pneumoniae

54
Q

✓Alpha hemolytic
✓Bile solubility(-) (insoluble)
✓ Optochin: Resistant
✓Neufeld Quellung: Negative (-)

A. S. pneumoniae
B. Viridians

A

tagumpay Sa MARCH 2024! (19 decks)

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