GRAM NEGATIVE COCCI Flashcards
GRAM NEGATIVE DIPLOCOCCI resembling COFFEE BEANS/KIDNEY BEANS
NON-MOTILE, OBLIGATE ANAEROBES
CAPNOPHILIC
CATALASE +
OXIDASE +
Neisseria
Note:
Catalase- , rod-shaped : N.elongata
Asymptomatic more common in Females
#2 leading cause of STD
GENITAL INFECTIONS:
Urethritis: Male
Cervicitis: Female
Other Localized infections:
Pharyngitis, anorectal infections, Conjunctivitis
Virulence:
Pili: for attachment
IgA protease
N. gonorrhoeae
Causes OPTHALMIA NEONATORIUM of Newborns
Causes:
Pelvic inflammatory disease/disseminated gonococcal infection includes BACTEREMIA, ARTHRITIS, METASTATIC INFECTIONS
N.gonorrhoeae
POLYSACCHARIDE CAPSULES:
A: PANDEMICS
B: COMMUNITY ACQUIRED = common cause of Meningitidis
Y: (PNEUMONIA), W35 (INVASIVE DISEASE)
Common in ADOLESCENT/YOUNG ADULT
LIFE-THREATENING, ACUTE, PURULENT MENINGITIS
N.meningitidis
Meningitidis may be accompanied by appearance of Petichiae (rash) that is associated with Meningococcal bacteremia (meningococcemia)
Bacteremia leads to:
THROMBOCYTOPENIA
DIC
SHOCK
N.meningitidis
Hemorrhage in Adrenal Gland
Treatment: Ceftriaxone
Waterhouse- Friderichsen Syndrome
Previous names:
Branhamella catarrhalis
Neisseria catarrhalis
Moraxella catarrhalis
Common cause of OTITIS MEDIA
Most Infections are localized assosciated with Respiratory tract and includes SINUSITIS AND PNEUMONIA
LOWER RESPIRATORY TRACT INFECTIONS targets the ELDERLY PATIENTS
M. catarrhalis
BAP +
CAP +
TMA+
N.meningitidis
Note: 35°c in a 3% to 5% CO2 atmosphere in 3 days/72hrs.
BAP -
CAP+
TMA+
N.gonorrhoeae
Note: 35°c in a 3% to 5% CO2 atmosphere in 3 days/72hrs.
BAP+
CAP+
TMA-
Moraxella
Note: 35°c in a 3% to 5% CO2 atmosphere in 3 days/72hrs.
Small, Grayish White, Convex, TRANSLUCENT, Shinny Colonies with either Smooth/irregular margins
N.gonorrhoeae
Medium, Smooth, round, moist, Gray to White; ENCAPSULATED strains are MUCOID
GREENISH CAST IN agar underneath colonies
N. meningitidis
CLAY-LIKE Consistency, Smooth, Glistening
Catalase -
Elongated rod shaped
N. elongata
friable “HOCKEY PUCK”
colony may be MOVED INTACT OVER SURFACE AGAR
M.catarrhalis
Oxidase Test (Taxo N) : Positive (purple)
Neisseria and Moraxella
Superoxol test (30%) H2O2: VIGOROUS BUBBLING
N.gonorrhoeae
Superoxol test (30%) H2O2: WEAK DELAYED BUBBLING
N. meningitidis and N. lactamica
GLUCOSE: -
MALTOSE: -
SUCROSE: -
LACTOSE: -
Moraxella catarrhalis
Note:
ASACCHAROLYTIC:
“hindi sweet si jason AMOA”
A- Acinetobacter iwoffi
M- Moraxella
O- Oligella
A- Alcaligenes
GLUCOSE: +
MALTOSE: -
SUCROSE: -
LACTOSE: -
N. gonorrhoeae
GLUCOSE: +
MALTOSE: +
SUCROSE: -
LACTOSE: -
N. meningitidis
GLUCOSE: +
MALTOSE: +
SUCROSE: +/-
LACTOSE: -
N. subflava
GLUCOSE: +
MALTOSE: +
SUCROSE: -
LACTOSE: +
N. lactamica
Note: SELECTIVE MEDIA FOR N.GONORRHEA
Thayer Martin
Modified Thayer Martin (MTM)
Martin Lewis
GC-LECT
New York City Agar
Acid Production characterized by YELLOW color Produced in 24-72 hrs.
Cystine trypticase agar (CTA)
Detects Enzyme that Hydrolizes colorless substrates and colored end products
Chromogenic Substrates
Hybridization, NAAT, MALDI-TOF
molecular assays
specimen for NAAT of N. gonorrhea and Chlamydia
Urethral Specimen; Urine (male/female)
Require specific nutritional factor in artificial media for growth to occur
Auxotypes
Requires arginine, uracil, hypoxanthine
Cause asymptomatic urethral infection in Males
AHU Strains
Rapid detection of Carbohydrate utilization and production of DNAse and beta lactamase
Multitest conventional (QuadFERM)
BACILLI
AEROBIC
OXIDIZER
CPON: CATALASE +
OXIDASE -
A. Acinetobacter
B. Capnocytophaga
C. Neisseria
D. Mycoplasma, Ureaplasma
E. Kingella
A. Acinetobacter
DIPLOCOCCI
FERMENTER
CAPNOPHILIC
NON-MOTILE
CPOP: CATALASE +
OXIDASE +
A. Acinetobacter
B. Capnocytophaga
C. Neisseria
D. Mycoplasma, Ureaplasma
E. Kingella
C. Neisseria
FUSIFORM SHAPE
GLIDING MOTILITY
CATALASE -
A. Acinetobacter
B. Capnocytophaga
C. Neisseria
D. Mycoplasma, Ureaplasma
E. Kingella
B. Capnocytophaga
SQUARE- ENDED ROD
TWITCHING MOTILITY
CATALASE -
A. Acinetobacter
B.Capnocytophaga
C. Neisseria
D. Mycoplasma, Ureaplasma
E. Kingella
E. Kingella
GROW ON NYC AGAR
CELL WALL; LESS PELOMORPHIC
A. Acinetobacter
B.Capnocytophaga
C. Neisseria
D. Mycoplasma, Ureaplasma
E. Kingella
D. Mycoplasma, Ureaplasma