CLINICAL CHEMISTRY Flashcards
Base SI unit for the amount of a substance
A. Mole(mol)
B. Millimole
C. Equivalent weight
A. Mole(mol)
Concentration expressed as the amount of solute per 100 parts of solution (%)
A. Percent solution
B. Molarity
C. Normality
D. Molality
A . Percent solution
Moles of solute per kilogram of solvent (mol/kg)
A. Percent solution
B. Molarity
C. Normality
D. Molality
D. Molality
Moles per liter of solution (mol/L)
A. Percent solution
B. Molarity
C. Normality
D. Molality
B. Molarity
Equivalent weight per liter of solution (Eq/L)
A. Percent solution
B. Molarity
C. Normality
D. Molality
C. Normality
Number of Osmoles of solute per liter of solution or per kilogram of solvent
A. SOLUTION
B. CONCENTRATION
C. OSMOLARITY OR OSMOLALITY
C. OSMOLARITY OR OSMOLALITY
A uniform mixture of solute and solvent and is described in terms of concentration
A. SOLUTION
B. CONCENTRATION
C. OSMOLARITY OR OSMOLALITY
A. SOLUTION
Amount of solute in a given volume of solution
A. SOLUTION
B. CONCENTRATION
C. OSMOLARITY OR OSMOLALITY
B. CONCENTRATION
Normal serum osmolality:
A. 270-280 mOsm/kg
B. 275-295 mOsm/kg
C. 275-290 mOsm/kg
B. 275-295 mOsm/kg
An expression of osmotic concentration; hypertonic, isotonic, hypotonic
A. Tonicity
B. Saturation
C. Colligative properties
A. Tonicity
Routine terms in clinical laboratory that describe the extent of saturation are dilute, concentrated, saturated, and supersaturated
A. Tonicity
B. Saturation
C. Colligative properties
B. Saturation
Physical properties of solution that depend on the relative concentration of solute and solvent but not on their identities
A. Tonicity
B. Saturation
C. Colligative properties
C. Colligative properties
PRESSURE THAT OPPOSES OSMOSIS WHEN THE SOLVENT FLOWS THROUGH A SEMIPERMEABLE MEMBRANE TO ESTABLISH EQUILIBRIUM BETWEEN COMPARTMENTS OF DIFFERING CONCENTRATION
INCREASED BY 1.7 * 10 ^ 4
A. VAPOR PRESSURE
B. FREEZING POINT
C. BOILING POINT
D. OSMOTIC PRESSURE
D. OSMOTIC PRESSURE
PRESSURE AT WHICH LIQUID SOLVENT IS THE EQUILIBRIUM WITH WATER VAPOR
DECREASED by 0.3 mmHg or torr
A. VAPOR PRESSURE
B. FREEZING POINT
C. BOILING POINT
D. OSMOTIC PRESSURE
A. VAPOR PRESSURE
TEMPERATURE AT WHICH THE VAPOR PRESSURE OF THE SOLID AND LIQUID PHASES OF A SUBSTANCE ARE THE SAME
DECREASED by 1.86°c
A. VAPOR PRESSURE
B. FREEZING POINT
C. BOILING POINT
D. OSMOTIC PRESSURE
B. FREEZING POINT
TEMPERATURE AT WHICH THE VAPOR PRESSURE OF THE SOLVENT REACHES 1 ATM
INCREASED BY 0.52°C
A. VAPOR PRESSURE
B. FREEZING POINT
C. BOILING POINT
D. OSMOTIC PRESSURE
C. BOILING POINT
✓Very high purity
✓ meets specifications of American Chemical Society
A. Analytic reagent
B. Ultra pure
C. Chemically pure
D. US Pharmacopia and National Formulatory
E. Technical or commercial
A. Analytic reagent
✓SPECTROGRADE, NANOGRADE or HPLC GRADE
✓ used for gas chromatography, HPLC, fluorometry, AAS, immunoassays, molecular diagnostics, trace metal determinations, standardization or other techniques that require pure chemical
A. Analytic reagent
B. Ultra pure
C. Chemically pure
D. US Pharmacopia and National Formulatory
E. Technical or commercial
B. Ultra pure
✓ Melting Point Analysis used to ascertain the acceptable purity range
✓ NOT RECOMMENDED FOR CLINICAL LABS but may be acceptable for some lab applications when higher purity chemicals aren’t available
A. Analytic reagent
B. Ultra pure
C. Chemically pure
D. US Pharmacopia and National Formulatory
E. Technical or commercial
C. Chemically pure
✓ Not injurious to health
✓ used to manufacture drugs
A. Analytic reagent
B. Ultra pure
C. Chemically pure
D. US Pharmacopia and National Formulatory
E. Technical or commercial
D. US Pharmacopia and National Formulatory
✓FOR INDUSTRIAL USE
✓not of sufficient purity to use as analytic reagents
A. Analytic reagent
B. Ultra pure
C. Chemically pure
D. US Pharmacopia and National Formulatory
E. Technical or commercial
E. Technical or commercial
✓Most commonly used glassware in the laboratory
✓High resistance to thermal shock and chemical attack. low alkali content
✓can be heated and autoclaved
strain point 515° c
Borosilicate glass
✓6 * stronger than borosilicate
Aliminosilicate glass (corex)
✓High thermal and heat shock resistance 900°c
✓ACID resistant
Vycor (Corning)
“ACIDVy”
✓Used for highly alkaline solutions
✓ALKALI resistant
✓Poor heat resistance
Boron free or soft glass
“Alkali-Booo”🤣
✓Excellent optical properties
✓Used for high precision analytic work, optical reflectors, mirrors
High silica
✓Used for some DISPOSABLE glassware
✓least expensive but poor resistance to high temperature
Flint glass
✓Used to decrease exposure to light
✓Amber/Red
Low actinic
✓Relatively inert chemically resistant to most acids, alkali, and salts
✓ can be autoclaved
✓USED FOR PIPETTE TIPS, TEST TUBES
Polypropylene
✓used for test tubes bottles, disposable transfer pipettes, test tube racks
✓CAN’T be AUTOCLAVED
Polyethylene
✓Stronger than polypropylene
✓ better temperature tolerance but chemical resistance not as good
✓USED FOR CENTRIFUGE TUBES GRADUATED CYLINDERS
Polycarbonate
✓Rigid,clear.
✓shouldn’t be autoclave
✓used for Test tubes, Graduated tubes
Polystyrene
✓frequently used as tubing
Polyvinyl chloride
Extremely inert
Teflon
Delivers the EXACT AMOUNT it holds into a container
To Deliver (TD)
Holds the particular volume but DOES NOT DISPENSE the EXACT amount
To contain (TC)
Meets high standards for accuracy
RECOMMEND FOR CALIBRATION
Class A (A)
✓ Has a continuous etched rings on top of the pipette;
✓ exact volume is obtained when the drop is blown out
✓ Viscous Fluids
Blowout
✓ Absence of etched rings on top of the pipette
✓ liquid is allowed to drain by gravity
✓ touch of last drop against wall of receiving vessel
✓ NON VISCOUS FLUIDS
Self-draining
Graduation marks to the tip and is generally BLOW OUT
Serologic
✓Does not have graduation to the tip
✓ SELF DRAINING
Mohr
✓ Bulb Is In The Middle
✓ designed to dispense or transfer aqueous solution and is always self draining
✓ greatest degree of accuracy and precision
✓ used when diluting standards, calibrators, or quality control material
A. VOLUMETRIC PIPET
B. OSTWALD-FOLIN
C. PASTEUR PIPET
D. AUTOMATIC MACROPIPETS OR MICROPIPETS
A. VOLUMETRIC PIPET
✓Bulb is nearer the mouthpiece
✓ blowout pipets, indicated by 2 etched continuous ring at the top
A. VOLUMETRIC PIPET
B. OSTWALD-FOLIN
C. PASTEUR PIPET
D. AUTOMATIC MACROPIPETS OR MICROPIPETS
B. OSTWALD-FOLIN
✓no calibration marks and are used to transfer solution or biologic fluids without consideration of a specific volume
✓ NOT TO BE USED IN ANY QUANTITATIVE ANALYTIC TECHNIQUES
A. VOLUMETRIC PIPET
B. OSTWALD-FOLIN
C. PASTEUR PIPET
D. AUTOMATIC MACROPIPETS OR MICROPIPETS
C. PASTEUR PIPET
MOST ROUTINELY USED PIPETTE IN TODAY’S CLINICAL CHEMISTRY LABORATORY
A. VOLUMETRIC PIPET
B. OSTWALD-FOLIN
C. PASTEUR PIPET
D. AUTOMATIC MACROPIPETS OR MICROPIPETS
D. AUTOMATIC MACROPIPETS OR MICROPIPETS
Produces a monolayer of cells
Cytocentrifuge
Force acting on sample being
RCF: Relative Centrifugal Force
Determined by tachometer or strobe light
rpm/revolutions per minute
✓ Tubes are in HORIZONTAL position when ROTATING and VERTICAL in REST
✓ recommended for Serum Separator Tubes
Horizontal head centrifuge (swinging-bucket)
✓ Tubes are at fixed angle 25° to 45° when rotating
✓ capable of higher speeds
Angle-head centrifuge
✓High speed capable of 100,000 rpm
✓Reference method for lipoproteins
Ultra centrifuge
Multiple test analyzed one after another on a given specimen
A. Sequential Analysis
B. Parallel testing
C. Batch analysis
D. Random Access
A. Sequential Analysis
Able to perform individual tests or panels, and allow for STAT samples to be added to the run ahead of other specimens; any test on any sample in any sequence
A. Sequential Analysis
B. Parallel testing
C. Batch analysis
D. Random Access
D. Random Access
All samples are loaded at the same time, and a single test is performed in each sample
A. Sequential Analysis
B. Parallel testing
C. Batch analysis
D. Random Access
C. Batch analysis
More than one test is analyzed concurrently on a given specimen
A. Sequential Analysis
B. Parallel testing
C. Batch analysis
D. Random Access
C. Parallel testing
System wherein reagents from other manufacturers can be used
A. Open reagent system
B. Closed reagent system
A. Open reagent system
Only the manufacturers reagent may be used
A. Open reagent system
B. Closed reagent system
B. Closed reagent system
Maximum number of tests generated per hour
A. Throughput
B. Turnaround
C. Dead volume
D. Carry over
E. Reflex testing
A. Throughput
Amount of time to generate one result
A. Throughput
B. Turnaround
C. Dead volume
D. Carry over
E. Reflex testing
B. Turnaround
Amount of serum that cannot be aspirated
A. Throughput
B. Turnaround
C. Dead volume
D. Carry over
E. Reflex testing
C. Dead volume
The contamination of a sample by a previous aspirated sample
A. Throughput
B. Turn around
C. Dead volume
D. Carry over
E. Reflex testing
D. Carry over
Use of preliminary test result to determine if additional test should be ordered or cancelled on a particular specimen; performed manually or automated
A. Throughput
B. Turnaround
C. Dead volume
D. Carry over
E. Reflex testing
E. Reflex testing
Produces an intense, reproducible, constant beam of polychromatic light
A. Light source/Exciter lamp
B. Entrance slit
C. Monochromator
D. Exit slit
A. Light source/Exciter lamp
Fixed in position and size to minimize unwanted or stray light and prevents scattered light into the monochromator system
A. Light source/Exciter lamp
B. Entrance slit
C. Monochromator
D. Exit slit
B. Entrance slit
Disperses the light into isolated wavelengths
A. Light source/Exciter lamp
B. Entrance slit
C. Monochromator
D. Exit slit
C. Monochromator
Select the bandpass of the selected wavelength to pass through the cuvet onto the detector
A. Light source/Exciter lamp
B. Entrance slit
C. Monochromator
D. Exit slit
D. Exit slit
Emits radiation that changes in intensity; Widely used
A. Continuum source
B. Line source
A. Continuum source
Emits limited radiation and wavelength
A. Continuum source
B. Line source
B. Line source
Most common used in visible and near infrared regions
A. Tungsten light bulb
B. Deuterium lamp
C. Xenon discharge lamp
A. Tungsten light bulb