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tagumpay Sa MARCH 2024! > MICROBIOLOGY MEDIUM2 > Flashcards

MICROBIOLOGY MEDIUM2 Flashcards

1
Q

Differential Isolation and Presumptive identification of Group D Streptococci and Enterococci

A

Bile esculin agar

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2
Q

Selective and Differential for cultivation of Vancomycin-resistant Enterococci from clinical and surveillance specimens

A

Bile esculin azide agar with Vancomycin

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3
Q

cultivation of fastidious microorganisms

A

Blood agar

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4
Q

francisella spp

A

Blood-Glucose-Cysteine medium

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5
Q

isolation of Bordetella pertussis and Bordetella parapertussis

A

Bordet-Gengou agar/Potato Blood Glycerol

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6
Q

cultivation of FASTIDIOUS ORGANISMS

A

Brain heart infusion agar or broth

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7
Q

Enrichment for Legionella spp.
Supports the growth of Francisella and Nocardia

A

Buffered charcoal-yeast extract agar (BCYE)

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8
Q

ENRICHMENT and SELECTION for Legionella spp.

A

Buffered charcoal-yeast extract agar (BCYE) with ANTIBIOTICS

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9
Q

for recovery of Burkholderia cepacia from cystic fibrosis patients

A

Burkholderia cepacia selective agar

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10
Q

Selective for Campylobacter spp.

A

Campy-blood agar

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11
Q

SELECTIVE HOLDING MEDIUM for recovery of Campylobacter spp.

A

Campylobacter thioglycollate broth

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12
Q

C. difficile

A

Cycloserine-Cefoxitine Fructose Agar

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13
Q

Improved growth of Obligate, slow-growing anaerobes

A

CDC anaerobe 5% sheepbblood agar

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14
Q

Selective medium for isolation of Campylobacter spp.

A

Cefoperazone, vancomycin, amphotericin (CVA) medium

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15
Q

Selective for Yersinia spp. May be useful for isolation of Aeromonas spp.

A

Cefsulodin-irgasan-novobiocin (CIN) agar

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16
Q

Cultivation of Haemophilus spp. And pathogenic Neisseria spp.

A

Chocolate agar

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17
Q

Designed to optimize growth and differentiate a specific type of organism. It is routinely used to identify YEAST, MRSA, and other organisms

A

Chromogenic agar

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18
Q

Selective isolation of GRAM-POSITIVE COCCI

A

Columbia colistin-nalidixic acid (CNA) agar

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19
Q

Isolation of C.diphtheriae

A

Cystine-tellurite blood agar

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20
Q

Isolation and differentiation of lactose-fermenting and non-lactose fermenting enteric bacilli

A

Eosin methylene blue (EMB) agar (Levine)

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21
Q

Leptospira interrogans

A

EMJH medium and fletcher medium

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22
Q

Legionella spp.

A

Feeley-Gorman medium

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23
Q

Selective (enrichment) liquid medium for enteric pathogens

A

Gram-negative (GN) broth

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24
Q

S.agalactiae

A

Granada medium

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25
Q

Differential, Selective medium for the isolation and differentiation of Salmonella and Shigella spp. From other Gram-Negative enteric bacilli

A

Hektoen enteric (HE) agar

26
Q

Borrelia spp.

A

Kelly’s medium

27
Q

ISOLATION AND GROWTH of Corynebacterium

A

Leoffler’s medium

28
Q

Egg-based medium for M.tubercolosis

A

Lowenstein-Jensen medium

29
Q

Isolation and Differentiation of Lactose fermenting and non-lactose fermenting enteric bacilli

A

MacConkey agar

30
Q

for the Isolation and Differentiation of E.coli O157:H7 in stool

A

MacConkey sorbitol agar

31
Q

SELECTIVE isolation of Staphylococci

A

Mannitol Salt Agar

32
Q

Listeria monocytogenes

A

McBride medium

33
Q

SELECTIVE for Neisseria gonorrhoeae; also supports growth of Ureaplasma urelyticum and Mycoplasma spp.

A

New York City (NYC) Agar

34
Q

Selective ISOLATION of all GRAM-POSITIVE cocci, AEROBIC GRAM-POSITIVE bacilli, and ANAEROBIC GRAM-NEGATIVE bacilli

A

Phenylethyl alcohol (PEA) agar

35
Q

ENRICHMENT and SELECTIVE medium for Isolation of Bordetella pertussis

A

Regan Lowe/Charcoal-Cephalexin Agar

36
Q

Selective for Salmonella and Shigella spp.

A

Salmonella-shigella (SS) agar

37
Q

NONSELECTIVE medium for the recovery of ANAEROBES and AEROBES

SELECTIVE for Campylobacter and Helicobacter spp.

A

Schaedler agar

38
Q

Enrichment of Isolation of Salmonella spp.

A

Selenite broth

39
Q

Mycoplasma

A

Shepard’s medium (SP4), A7B, E-agar

40
Q

Selective for Campylobacter spp

A

Skirrow agar

41
Q

Selective for Streptococcus pyogenes and Streptococcus agalactiae

(Contains Crystal violet, Colistin and STX)

A

Streptococcal Selective Agar (SSA)

42
Q

Selective for Salmonella and Shigella spp. EXCEPT S. typhi

A

Tetrathionate broth

43
Q

Selective for N. gonorrhoeae and N. meningitidis
Supports growth of Francisella and Brucella spp.

A

Thayer-Martin agar

44
Q

Supports growth of ANAEROBES, AEROBES, MICROAEROPHILIC, and FASTIDIOUS MICROORGANISMS

A

Thioglycollate broth

45
Q

SELECTIVE AND DIFFERENTIAL for Vibrios

A

Thiosulfate citrate-bile salts (TCBS) agar

46
Q

Selection and Enrichment for Streptococcus agalactiae in Female genital specimens

A

Todd-Hewitt broth with Antibiotics (LIM)

47
Q

Lactobacillus acidophilus

A

Tomato Juice agar

48
Q

Enrichment broth used for subculturing various bacteria from Primary Agar Plates

A

Trypticase Soy Broth (TSB)

49
Q

Isolation and Differentiation of Salmonella and Shigella spp. From other gram-negative enteric bacilli

A

Xylose Lysine Desoxycholate (XLD) agar

50
Q

S.pneumoniae

A

Gentamicin BAP (GBAP)

51
Q

H.influenzae

A

Bacitracin CAP (BCAP)

52
Q

Neisseria (for confirmatory)

A

Cystine Trypticase Agar

53
Q

C. trachomatis

A

McCoy Cells

54
Q

Brucella spp.

55
Q

To differentiate Aerobic Actinomycetes (Nocardia, Rhodococcus,Gordonia,Tsukamurella,Streptomyces, Actinomadura, Dermatophilus, Nocardiopsis)

A

Tap Water agar

Note: Bacto Agar is added to 100 mL of Tap Water, Sterilized and then poured into plates. Plates are inoculated and incubated @30°C for 7 Days, and examined daily for AERIAL HYPHAE

56
Q

Media Sterilization:
Autoclave @ _______for _____

A

121°C for 15 mins

57
Q

Media Sterilization:
Membrane filtration____ to____
for Heat-labile components

A

0.2 to 0.45um

58
Q

Urine cultures and tissues from Burn victims are Plated _________

A

Quantitatively (Cross-Streaking)

59
Q

Vancomycin+Colistin+ Nystatin+ TRIMETHOPRIM LACTATE which inhibits Swarming Proteus

A

Modified Thayer Martin (MTM)

60
Q

Vancomycin+Colistin+ ANISOMYCIN (Fungi/yeast)+ TRIMETHOPRIM LACTATE which inhibits Swarming Proteus

A

MARTIN LEWIS

61
Q

Vancomycin+LINCOMYCIN+ Colistin+ TRIMETHOPRIM LACTATE which inhibits Swarming Proteus + ANPHOTERICIN B (fungi)

62
Q

LYSED HORSE BLOOD+ Vancomycin+Colistin+AMPHOTERICIN B+ TRIMETHOPRIM

A

New York City Agar

tagumpay Sa MARCH 2024! (19 decks)

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