MICROBIOLOGY MEDIUM2 Flashcards

1
Q

Differential Isolation and Presumptive identification of Group D Streptococci and Enterococci

A

Bile esculin agar

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2
Q

Selective and Differential for cultivation of Vancomycin-resistant Enterococci from clinical and surveillance specimens

A

Bile esculin azide agar with Vancomycin

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3
Q

cultivation of fastidious microorganisms

A

Blood agar

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4
Q

francisella spp

A

Blood-Glucose-Cysteine medium

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5
Q

isolation of Bordetella pertussis and Bordetella parapertussis

A

Bordet-Gengou agar/Potato Blood Glycerol

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6
Q

cultivation of FASTIDIOUS ORGANISMS

A

Brain heart infusion agar or broth

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7
Q

Enrichment for Legionella spp.
Supports the growth of Francisella and Nocardia

A

Buffered charcoal-yeast extract agar (BCYE)

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8
Q

ENRICHMENT and SELECTION for Legionella spp.

A

Buffered charcoal-yeast extract agar (BCYE) with ANTIBIOTICS

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9
Q

for recovery of Burkholderia cepacia from cystic fibrosis patients

A

Burkholderia cepacia selective agar

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10
Q

Selective for Campylobacter spp.

A

Campy-blood agar

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11
Q

SELECTIVE HOLDING MEDIUM for recovery of Campylobacter spp.

A

Campylobacter thioglycollate broth

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12
Q

C. difficile

A

Cycloserine-Cefoxitine Fructose Agar

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13
Q

Improved growth of Obligate, slow-growing anaerobes

A

CDC anaerobe 5% sheepbblood agar

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14
Q

Selective medium for isolation of Campylobacter spp.

A

Cefoperazone, vancomycin, amphotericin (CVA) medium

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15
Q

Selective for Yersinia spp. May be useful for isolation of Aeromonas spp.

A

Cefsulodin-irgasan-novobiocin (CIN) agar

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16
Q

Cultivation of Haemophilus spp. And pathogenic Neisseria spp.

A

Chocolate agar

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17
Q

Designed to optimize growth and differentiate a specific type of organism. It is routinely used to identify YEAST, MRSA, and other organisms

A

Chromogenic agar

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18
Q

Selective isolation of GRAM-POSITIVE COCCI

A

Columbia colistin-nalidixic acid (CNA) agar

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19
Q

Isolation of C.diphtheriae

A

Cystine-tellurite blood agar

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20
Q

Isolation and differentiation of lactose-fermenting and non-lactose fermenting enteric bacilli

A

Eosin methylene blue (EMB) agar (Levine)

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21
Q

Leptospira interrogans

A

EMJH medium and fletcher medium

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22
Q

Legionella spp.

A

Feeley-Gorman medium

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23
Q

Selective (enrichment) liquid medium for enteric pathogens

A

Gram-negative (GN) broth

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24
Q

S.agalactiae

A

Granada medium

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25
Differential, Selective medium for the isolation and differentiation of Salmonella and Shigella spp. From other Gram-Negative enteric bacilli
Hektoen enteric (HE) agar
26
Borrelia spp.
Kelly's medium
27
ISOLATION AND GROWTH of Corynebacterium
Leoffler's medium
28
Egg-based medium for M.tubercolosis
Lowenstein-Jensen medium
29
Isolation and Differentiation of Lactose fermenting and non-lactose fermenting enteric bacilli
MacConkey agar
30
for the Isolation and Differentiation of E.coli O157:H7 in stool
MacConkey sorbitol agar
31
SELECTIVE isolation of Staphylococci
Mannitol Salt Agar
32
Listeria monocytogenes
McBride medium
33
SELECTIVE for Neisseria gonorrhoeae; also supports growth of Ureaplasma urelyticum and Mycoplasma spp.
New York City (NYC) Agar
34
Selective ISOLATION of all GRAM-POSITIVE cocci, AEROBIC GRAM-POSITIVE bacilli, and ANAEROBIC GRAM-NEGATIVE bacilli
Phenylethyl alcohol (PEA) agar
35
ENRICHMENT and SELECTIVE medium for Isolation of Bordetella pertussis
Regan Lowe/Charcoal-Cephalexin Agar
36
Selective for Salmonella and Shigella spp.
Salmonella-shigella (SS) agar
37
NONSELECTIVE medium for the recovery of ANAEROBES and AEROBES SELECTIVE for Campylobacter and Helicobacter spp.
Schaedler agar
38
Enrichment of Isolation of Salmonella spp.
Selenite broth
39
Mycoplasma
Shepard's medium (SP4), A7B, E-agar
40
Selective for Campylobacter spp
Skirrow agar
41
Selective for Streptococcus pyogenes and Streptococcus agalactiae (Contains Crystal violet, Colistin and STX)
Streptococcal Selective Agar (SSA)
42
Selective for Salmonella and Shigella spp. EXCEPT S. typhi
Tetrathionate broth
43
Selective for N. gonorrhoeae and N. meningitidis Supports growth of Francisella and Brucella spp.
Thayer-Martin agar
44
Supports growth of ANAEROBES, AEROBES, MICROAEROPHILIC, and FASTIDIOUS MICROORGANISMS
Thioglycollate broth
45
SELECTIVE AND DIFFERENTIAL for Vibrios
Thiosulfate citrate-bile salts (TCBS) agar
46
Selection and Enrichment for Streptococcus agalactiae in Female genital specimens
Todd-Hewitt broth with Antibiotics (LIM)
47
Lactobacillus acidophilus
Tomato Juice agar
48
Enrichment broth used for subculturing various bacteria from Primary Agar Plates
Trypticase Soy Broth (TSB)
49
Isolation and Differentiation of Salmonella and Shigella spp. From other gram-negative enteric bacilli
Xylose Lysine Desoxycholate (XLD) agar
50
S.pneumoniae
Gentamicin BAP (GBAP)
51
H.influenzae
Bacitracin CAP (BCAP)
52
Neisseria (for confirmatory)
Cystine Trypticase Agar
53
C. trachomatis
McCoy Cells
54
Brucella spp.
TSB
55
To differentiate Aerobic Actinomycetes (Nocardia, Rhodococcus,Gordonia,Tsukamurella,Streptomyces, Actinomadura, Dermatophilus, Nocardiopsis)
Tap Water agar Note: Bacto Agar is added to 100 mL of Tap Water, Sterilized and then poured into plates. Plates are inoculated and incubated @30°C for 7 Days, and examined daily for AERIAL HYPHAE
56
Media Sterilization: Autoclave @ _______for _____
121°C for 15 mins
57
Media Sterilization: Membrane filtration____ to____ for Heat-labile components
0.2 to 0.45um
58
Urine cultures and tissues from Burn victims are Plated _________
Quantitatively (Cross-Streaking)
59
Vancomycin+Colistin+ Nystatin+ TRIMETHOPRIM LACTATE which inhibits Swarming Proteus
Modified Thayer Martin (MTM)
60
Vancomycin+Colistin+ ANISOMYCIN (Fungi/yeast)+ TRIMETHOPRIM LACTATE which inhibits Swarming Proteus
MARTIN LEWIS
61
Vancomycin+LINCOMYCIN+ Colistin+ TRIMETHOPRIM LACTATE which inhibits Swarming Proteus + ANPHOTERICIN B (fungi)
GC-LECT
62
LYSED HORSE BLOOD+ Vancomycin+Colistin+AMPHOTERICIN B+ TRIMETHOPRIM
New York City Agar