Clinical Microscopy Flashcards

1
Q

Perform the INITIAL MAGNIFICATION of the object on the mechanical stage

A

Objective

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2
Q

FURTHER MAGNIFIED object that has been enhanced by the objectives for viewing

A

Ocular (eyepiece)

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3
Q

Moves the MECHANICAL STAGE noticeably up and down until the object comes into view for initial focusing

A

Course adjustment knob

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4
Q

Adjustment to SHARPEN THE IMAGE

A

Fine adjustment knob

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5
Q

✓ Illumination for the modern microscope
✓equipped with RHEOSTAT to regulate the intensity of the light
✓filters may also be placed to vary the elimination and wavelengths of the emitted light

A

Light source

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6
Q

FOCUSES the LIGHT on the SPECIMEN and controls the light for uniform illumination

A

Condenser

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7
Q

CONTROLS THE AMOUNT OF LIGHT and the ANGLE OF LIGHT RAYS that pass to the specimen and lens, which affects resolution, contrast, and depth of the field of image;

A

Aperture (Iris) diaphragm

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8
Q

CONTROLS THE DIAMETER of the LIGHT BEAM reaching the slide and is adjusted for optimal illumination

A

Field diaphragm

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9
Q

✓Identifies the WBCs, EPITHELIAL CELLS, AND CASTS
✓Delineates structure and contrasting colors of the NUCLEUS AND CYTOPLASM
✓Crystal Violet and Safranin O

A

Sternheimer-Malbin

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10
Q

✓ Enhances nuclear detail
✓ Differentiate WBC’s and Renal Tubular Epithelial Cells

A

Toluidine blue

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11
Q

✓ Stains TRIGLYCERIDES and NEUTRAL FATS ORANGE-RED
✓Identifies Free Fat Droplets and Lipid-containing Cells and Casts

A

Lipid stains: Oil Red O and Sudan III

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12
Q

✓ Differentiates Gram-positive and Gram-negative Bacteria
✓ IDENTIFIES BACTERIAP CASTS

A

Gram stain

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13
Q

✓ Methylene Blue and eosin Y stain eosinophilic granules
✓ identifies Urinary Eosinophils

A

Hansel stain

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14
Q

✓ Stain structures containing iron
✓ identifies yellow-brown granules of hemosiderin in cells and casts

A

Prussian blue stain

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15
Q

Simple stain used to enhance nuclear details of epithelial cells in urine

A

Methylene blue

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16
Q

NOT a simple stain

A

KOVA’s stain

17
Q

✓ Yellow brown granules seen in CELLS AND CASTS following episodes of hemoglobinuria present 2-3 days after hemolytic event

✓ (+) Prussian blue Staining

A

Hemosiderin

18
Q

In automated microscopy, the DNA within the cells is stained by an orange dye

A

PHENATHRIDINE

19
Q

In automated microscopy, the nuclear membranes, mitochondria, and negatively charged cell membranes are stained by the green dye

A

Carbocyanine