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tagumpay Sa MARCH 2024! > MICROBIOLOGY:STERILIZATION > Flashcards

MICROBIOLOGY:STERILIZATION Flashcards

1
Q

INFECTIOUS MEDICAL WASTE IF OFTEN STERILIZED AT____ °C FOR__TO__ MINS.

A

132°C (270 °F); 30-60 MINS

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2
Q

CULTURE MEDIA HOW TO STERILIZE:

A

AUTOCLAVE OR MEMBRANE FILTRATION

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3
Q

EVACUATED TUBES STERILIZE THROUGH:

A

GAMMA RADIATION

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4
Q

MOST COMMON CHEMICAL STERILANT; COLD STERILIZATION

A. GLUTARALDEHYDE
B. PERACETIC ACID
C. ETHYLENE OXIDE

A

C.

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5
Q

SPORICIDAL IN 3 TO 10 HOURS, USED FOR MEDICAL EQUIPMENT SUCH AS BRONCHOSCOPES
A. GLUTARALDEHYDE
B. PERACETIC ACID
C. ETHYLENE OXIDE

A

A

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6
Q

PROCESS THAT KILLS ALL FORMS OF MICROBIAL LIFE, INCLUDING BACTERIAL SPORES
A. DISINFECTION
B. STERILIZATION
C. AUTOCLAVE

A

B.

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7
Q

PROCESS THAT DESTROYS PATHOGENIC ORGANISMS, BUT NOT NECESSARILY ALL MICROORGANISMS OR SPORES

A. DISINFECTION
B. STERILIZATION
C. AUTOCLAVE

A

A

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8
Q

LONG WAVELENGTH AND LOW ENERGY (EXAMPLE: MERCURY LAMP)

A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION

A

C.

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9
Q

100C FOR15 MINS. WHICH KILLS VEGETATIVE BACTERIA
A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION

A

B.

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10
Q

USED MOSTLY IN FOOD INDUSTRY, ELIMINATES FOOD-BORNE PATHOGENS AND ORGANISMS RESPONSIBLE FOR FOOD SPOILAGE:

A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION

A

A.

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11
Q

DESTOYS VEGETATIVE FORMS ON INANIMATE OBJECTS
A. DISINFECTANT
B. ANTISEPTIC

A

A

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12
Q

MICROBIOSTATIC AGENTS USED ON LIVING TISSUE
A. DISINFECTANT
B. ANTISEPTIC

A

B.

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13
Q

IODINE+ DETERGENT

A

IODOPHOR

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14
Q

IODINE IN ALCOHOL

A

TINCTURE

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15
Q

PREVENTION OF OPTHALMIA NEONATORIUM

A

CREDE’S PROPHYLAXIS OR APPLYING OF 1% SILVER NITRATE

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16
Q

CAUSATIVE AGENT OF OPHTHALMIA NEONATORIUM

A

N. GONORRHOEAE

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17
Q

USED TO DISINFECT BENCH TOPS OR OTHER SURFACES IN THE LABORATORY

CAN BE ACTIVATED BY ORGANIC SUBSTANCE

A

QUATS: QUATERNARY AMMONIUM COMPOUNDS

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18
Q

STANDARD DISINFECTANT IN LABORATORY;
EXPRESSION OF THE BACTERICIDAL POWER OF PARTIULAR SUBSTANCE AS COMPARED TO PURE PHENOL

A

PHENOL COEFFICIENT

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19
Q

BIOLOGICAL NDICATORS OF AUTOCLAVE

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

A

A.

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20
Q

BIOLOGICAL NDICATORS OF ETHYLENE OXIDE

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

A

D

21
Q

BIOLOGICAL INDICATORS OF IONIZING RADIATION

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

A

B.

22
Q

BIOLOGICAL NDICATORS OF DRY HEAT OVEN

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

A

C.

23
Q

NO RECIRCULATION

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

G.CLASS II B2

24
Q

HIGHEST LEVEL OF SAFETY

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

H. CLASS III

25
Q

SELECTED IF RADIOISOTOPES, TOXIC CHEMICALS, OR CARCINOGENS WILL BE USED;
AIR IS DISCHARGE OUTSIDE THE BUILDING
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

E.CLASS II B

26
Q

30% RECIRCULATED 70% EXHAUSTED

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

F. CLASS II B1

27
Q

ONLY THE AIR TO BE EXHAUSTED

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

A. CLASS I

28
Q

MOST COMMON; ALSO CALLED AS LAMINAR FLOW BSC; STERILIZE BOTH EXHAUST AND CIRCULATING AIR
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

B. CLASS IIA

29
Q

70% OF AIR IS RECIRCULATED

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

A

C. CLASS II A1

30
Q

MEASLES, VARICELLA, TUBERCOLOSIS, SMALL POX

A

AIRBORNE, USE SNUG FIT MASK/ N95

31
Q

H.INFLUENZAE TYPE B INFECTION, INCLUDING MENINGITIS, PNEUMONIA, EPIGLOTTITIS, SEPSIS

A

DROPLET USE SURGICAL MASK

32
Q

COMMON CAUSE OF DIARRHEA, CONTACT PRECAUTION IS ADVICE

A

C. DIFFICLE

33
Q

A process whereby all forms of microbial life including bacterial spores are killed

A

Sterilization (BOICIDE)

34
Q

Coagulates proteins

A

Moist heat

35
Q

Most effective method for sterilization and waste disposal

121° c at 15 lbs pressure per square inch for 15 minutes steam under pressure

Kills viruses and bacteria

A

Autoclave

36
Q

Autoclave is used to sterilize the following:
liquid media, instruments, glasswares at:

A 121° c for 15 minutes
B 125° c for 15 minutes
C 121° c for 30 minutes
D125° c for 30 minutes

A

A. 121° c (250 F) for 15 minutes

37
Q

Infectious medical waste at_____ for _____minutes allows penetration of the steam throughout the waste and the displacement of air trapped inside the autoclave bag

A. 131° c for 30 to 60 minutes
B. 132° c for 30 to 60 minutes
C. 131° c for 15 to 30 minutes
D. 132° c for 15 to 30 minutes

A

B 132°c (270 F) for 30 to 60 minutes

38
Q

Flowing steam 100° c for 30 minutes for three successive days

A. Tyndallization
B. Inspissation

A

A. Tyndallization

39
Q

Used for culture media with increased protein
75° c to 80° c for 2 hours for 3 successive days

A. Tyndallization
B. Inspissation

A

B. Inspissation

40
Q

Use for sterilizing wire loops and inoculating needles

A

Flame

41
Q

Used for glasswares, certain metals, oils, petrolatum, and powders.

160° c to 180° c for 1 and a half to 3 hours

A

Oven

42
Q

MOST COMMON method of treating infectious waste,
SAFEST method to ensure that no infective materials remain in samples are containers when disposed

A

Incineration

Feedback:
PRIONS, infective proteins are NOT ELIMINATED using conventional methods hence INCINERATION is recommended

43
Q

Used for controlling disease

A

Cremation

44
Q

Quality control for autoclave

A. Spores of Bacillus subtilis
B. Spores of Bacillus thearothermophilus

A

B. Spores of Bacillus thearothermophilus

45
Q

Quality control for oven

A. Spores of Bacillus subtilis
B. Spores of Bacillus thearothermophilus

A

A. Spores of Bacillus subtilis

46
Q

Is a process whereby pathogenic organisms, but not necessarily all microorganisms or spores are destroyed

A

DISINFECTION (ANTI-SEPSIS)

47
Q

Disinfectants used on living tissue or skin are called

A

Antiseptics

48
Q

Disinfectants at high concentrations used in soaps at low concentrations

A

Phenolics

Example: phenol, carbolic acid, lysol, hexachlorophene

tagumpay Sa MARCH 2024! (19 decks)

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