MICROBIOLOGY:STERILIZATION

Question 1

INFECTIOUS MEDICAL WASTE IF OFTEN STERILIZED AT____ °C FOR__TO__ MINS.

Answer 1

132°C (270 °F); 30-60 MINS

Question 2

CULTURE MEDIA HOW TO STERILIZE:

Answer 2

AUTOCLAVE OR MEMBRANE FILTRATION

Question 3

EVACUATED TUBES STERILIZE THROUGH:

Answer 3

GAMMA RADIATION

Question 4

MOST COMMON CHEMICAL STERILANT; COLD STERILIZATION

A. GLUTARALDEHYDE
B. PERACETIC ACID
C. ETHYLENE OXIDE

Answer 4

C.

Question 5

SPORICIDAL IN 3 TO 10 HOURS, USED FOR MEDICAL EQUIPMENT SUCH AS BRONCHOSCOPES
A. GLUTARALDEHYDE
B. PERACETIC ACID
C. ETHYLENE OXIDE

Answer 5

A

Question 6

PROCESS THAT KILLS ALL FORMS OF MICROBIAL LIFE, INCLUDING BACTERIAL SPORES
A. DISINFECTION
B. STERILIZATION
C. AUTOCLAVE

Answer 6

B.

Question 7

PROCESS THAT DESTROYS PATHOGENIC ORGANISMS, BUT NOT NECESSARILY ALL MICROORGANISMS OR SPORES

A. DISINFECTION
B. STERILIZATION
C. AUTOCLAVE

Answer 7

A

Question 8

LONG WAVELENGTH AND LOW ENERGY (EXAMPLE: MERCURY LAMP)

A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION

Answer 8

C.

Question 9

100C FOR15 MINS. WHICH KILLS VEGETATIVE BACTERIA
A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION

Answer 9

B.

Question 10

USED MOSTLY IN FOOD INDUSTRY, ELIMINATES FOOD-BORNE PATHOGENS AND ORGANISMS RESPONSIBLE FOR FOOD SPOILAGE:

A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION

Answer 10

A.

Question 11

DESTOYS VEGETATIVE FORMS ON INANIMATE OBJECTS
A. DISINFECTANT
B. ANTISEPTIC

Answer 11

A

Question 12

MICROBIOSTATIC AGENTS USED ON LIVING TISSUE
A. DISINFECTANT
B. ANTISEPTIC

Answer 12

B.

Question 13

IODINE+ DETERGENT

Answer 13

IODOPHOR

Question 14

IODINE IN ALCOHOL

Answer 14

TINCTURE

Question 15

PREVENTION OF OPTHALMIA NEONATORIUM

Answer 15

CREDE’S PROPHYLAXIS OR APPLYING OF 1% SILVER NITRATE

Question 16

CAUSATIVE AGENT OF OPHTHALMIA NEONATORIUM

Answer 16

N. GONORRHOEAE

Question 17

USED TO DISINFECT BENCH TOPS OR OTHER SURFACES IN THE LABORATORY

CAN BE ACTIVATED BY ORGANIC SUBSTANCE

Answer 17

QUATS: QUATERNARY AMMONIUM COMPOUNDS

Question 18

STANDARD DISINFECTANT IN LABORATORY;
EXPRESSION OF THE BACTERICIDAL POWER OF PARTIULAR SUBSTANCE AS COMPARED TO PURE PHENOL

Answer 18

PHENOL COEFFICIENT

Question 19

BIOLOGICAL NDICATORS OF AUTOCLAVE

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

Answer 19

A.

Question 20

BIOLOGICAL NDICATORS OF ETHYLENE OXIDE

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

Answer 20

D

Question 21

BIOLOGICAL INDICATORS OF IONIZING RADIATION

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

Answer 21

B.

Question 22

BIOLOGICAL NDICATORS OF DRY HEAT OVEN

A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII

Answer 22

C.

Question 23

NO RECIRCULATION

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

Answer 23

G.CLASS II B2

Question 24

HIGHEST LEVEL OF SAFETY

A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III

Answer 24

H. CLASS III

Question 25

SELECTED IF RADIOISOTOPES, TOXIC CHEMICALS, OR CARCINOGENS WILL BE USED; AIR IS DISCHARGE OUTSIDE THE BUILDING A. CLASS I B. CLASS II A C. CLASS II A1 D. CLASS II A2 E. CLASS II B F. CLASS II B1 G. CLASS II B2 H. CLASS III

Answer 25

E.CLASS II B

Question 26

30% RECIRCULATED 70% EXHAUSTED A. CLASS I B. CLASS II A C. CLASS II A1 D. CLASS II A2 E. CLASS II B F. CLASS II B1 G. CLASS II B2 H. CLASS III

Answer 26

F. CLASS II B1

Question 27

ONLY THE AIR TO BE EXHAUSTED A. CLASS I B. CLASS II A C. CLASS II A1 D. CLASS II A2 E. CLASS II B F. CLASS II B1 G. CLASS II B2 H. CLASS III

Answer 27

A. CLASS I

Question 28

MOST COMMON; ALSO CALLED AS LAMINAR FLOW BSC; STERILIZE BOTH EXHAUST AND CIRCULATING AIR A. CLASS I B. CLASS II A C. CLASS II A1 D. CLASS II A2 E. CLASS II B F. CLASS II B1 G. CLASS II B2 H. CLASS III

Answer 28

B. CLASS IIA

Question 29

70% OF AIR IS RECIRCULATED A. CLASS I B. CLASS II A C. CLASS II A1 D. CLASS II A2 E. CLASS II B F. CLASS II B1 G. CLASS II B2 H. CLASS III

Answer 29

C. CLASS II A1

Question 30

MEASLES, VARICELLA, TUBERCOLOSIS, SMALL POX

Answer 30

AIRBORNE, USE SNUG FIT MASK/ N95

Question 31

H.INFLUENZAE TYPE B INFECTION, INCLUDING MENINGITIS, PNEUMONIA, EPIGLOTTITIS, SEPSIS

Answer 31

DROPLET USE SURGICAL MASK

Question 32

COMMON CAUSE OF DIARRHEA, CONTACT PRECAUTION IS ADVICE

Answer 32

C. DIFFICLE

Question 33

A process whereby all forms of microbial life including bacterial spores are killed

Answer 33

Sterilization (BOICIDE)

Question 34

Coagulates proteins

Answer 34

Moist heat

Question 35

Most effective method for sterilization and waste disposal 121° c at 15 lbs pressure per square inch for 15 minutes steam under pressure Kills viruses and bacteria

Answer 35

Autoclave

Question 36

Autoclave is used to sterilize the following: liquid media, instruments, glasswares at: A 121° c for 15 minutes B 125° c for 15 minutes C 121° c for 30 minutes D125° c for 30 minutes

Answer 36

A. 121° c (250 F) for 15 minutes

Question 37

Infectious medical waste at_____ for _____minutes allows penetration of the steam throughout the waste and the displacement of air trapped inside the autoclave bag A. 131° c for 30 to 60 minutes B. 132° c for 30 to 60 minutes C. 131° c for 15 to 30 minutes D. 132° c for 15 to 30 minutes

Answer 37

B 132°c (270 F) for 30 to 60 minutes

Question 38

Flowing steam 100° c for 30 minutes for three successive days A. Tyndallization B. Inspissation

Answer 38

A. Tyndallization

Question 39

Used for culture media with increased protein 75° c to 80° c for 2 hours for 3 successive days A. Tyndallization B. Inspissation

Answer 39

B. Inspissation

Question 40

Use for sterilizing wire loops and inoculating needles

Answer 40

Flame

Question 41

Used for glasswares, certain metals, oils, petrolatum, and powders. 160° c to 180° c for 1 and a half to 3 hours

Answer 41

Oven

Question 42

MOST COMMON method of treating infectious waste, SAFEST method to ensure that no infective materials remain in samples are containers when disposed

Answer 42

Incineration Feedback: PRIONS, infective proteins are NOT ELIMINATED using conventional methods hence INCINERATION is recommended

Question 43

Used for controlling disease

Answer 43

Cremation

Question 44

Quality control for autoclave A. Spores of Bacillus subtilis B. Spores of Bacillus thearothermophilus

Answer 44

B. Spores of Bacillus thearothermophilus

Question 45

Quality control for oven A. Spores of Bacillus subtilis B. Spores of Bacillus thearothermophilus

Answer 45

A. Spores of Bacillus subtilis

Question 46

Is a process whereby pathogenic organisms, but not necessarily all microorganisms or spores are destroyed

Answer 46

DISINFECTION (ANTI-SEPSIS)

Question 47

Disinfectants used on living tissue or skin are called

Answer 47

Antiseptics

Question 48

Disinfectants at high concentrations used in soaps at low concentrations

Answer 48

Phenolics Example: phenol, carbolic acid, lysol, hexachlorophene