MICROBIOLOGY:STERILIZATION Flashcards
INFECTIOUS MEDICAL WASTE IF OFTEN STERILIZED AT____ °C FOR__TO__ MINS.
132°C (270 °F); 30-60 MINS
CULTURE MEDIA HOW TO STERILIZE:
AUTOCLAVE OR MEMBRANE FILTRATION
EVACUATED TUBES STERILIZE THROUGH:
GAMMA RADIATION
MOST COMMON CHEMICAL STERILANT; COLD STERILIZATION
A. GLUTARALDEHYDE
B. PERACETIC ACID
C. ETHYLENE OXIDE
C.
SPORICIDAL IN 3 TO 10 HOURS, USED FOR MEDICAL EQUIPMENT SUCH AS BRONCHOSCOPES
A. GLUTARALDEHYDE
B. PERACETIC ACID
C. ETHYLENE OXIDE
A
PROCESS THAT KILLS ALL FORMS OF MICROBIAL LIFE, INCLUDING BACTERIAL SPORES
A. DISINFECTION
B. STERILIZATION
C. AUTOCLAVE
B.
PROCESS THAT DESTROYS PATHOGENIC ORGANISMS, BUT NOT NECESSARILY ALL MICROORGANISMS OR SPORES
A. DISINFECTION
B. STERILIZATION
C. AUTOCLAVE
A
LONG WAVELENGTH AND LOW ENERGY (EXAMPLE: MERCURY LAMP)
A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION
C.
100C FOR15 MINS. WHICH KILLS VEGETATIVE BACTERIA
A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION
B.
USED MOSTLY IN FOOD INDUSTRY, ELIMINATES FOOD-BORNE PATHOGENS AND ORGANISMS RESPONSIBLE FOR FOOD SPOILAGE:
A. PASTEURIZATION
B. BOILING
C. NON-IONIZING RADIATION
A.
DESTOYS VEGETATIVE FORMS ON INANIMATE OBJECTS
A. DISINFECTANT
B. ANTISEPTIC
A
MICROBIOSTATIC AGENTS USED ON LIVING TISSUE
A. DISINFECTANT
B. ANTISEPTIC
B.
IODINE+ DETERGENT
IODOPHOR
IODINE IN ALCOHOL
TINCTURE
PREVENTION OF OPTHALMIA NEONATORIUM
CREDE’S PROPHYLAXIS OR APPLYING OF 1% SILVER NITRATE
CAUSATIVE AGENT OF OPHTHALMIA NEONATORIUM
N. GONORRHOEAE
USED TO DISINFECT BENCH TOPS OR OTHER SURFACES IN THE LABORATORY
CAN BE ACTIVATED BY ORGANIC SUBSTANCE
QUATS: QUATERNARY AMMONIUM COMPOUNDS
STANDARD DISINFECTANT IN LABORATORY;
EXPRESSION OF THE BACTERICIDAL POWER OF PARTIULAR SUBSTANCE AS COMPARED TO PURE PHENOL
PHENOL COEFFICIENT
BIOLOGICAL NDICATORS OF AUTOCLAVE
A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII
A.
BIOLOGICAL NDICATORS OF ETHYLENE OXIDE
A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII
D
BIOLOGICAL INDICATORS OF IONIZING RADIATION
A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII
B.
BIOLOGICAL NDICATORS OF DRY HEAT OVEN
A. B. STEAROTHERMOPHILUS
B. B. PUMILUS
C. BACILLUS SUBTILIS VAR. NIGER
D. BACILLUS SUBTILIS VAR. GLOBIGII
C.
NO RECIRCULATION
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
G.CLASS II B2
HIGHEST LEVEL OF SAFETY
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
H. CLASS III
SELECTED IF RADIOISOTOPES, TOXIC CHEMICALS, OR CARCINOGENS WILL BE USED;
AIR IS DISCHARGE OUTSIDE THE BUILDING
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
E.CLASS II B
30% RECIRCULATED 70% EXHAUSTED
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
F. CLASS II B1
ONLY THE AIR TO BE EXHAUSTED
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
A. CLASS I
MOST COMMON; ALSO CALLED AS LAMINAR FLOW BSC; STERILIZE BOTH EXHAUST AND CIRCULATING AIR
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
B. CLASS IIA
70% OF AIR IS RECIRCULATED
A. CLASS I
B. CLASS II A
C. CLASS II A1
D. CLASS II A2
E. CLASS II B
F. CLASS II B1
G. CLASS II B2
H. CLASS III
C. CLASS II A1
MEASLES, VARICELLA, TUBERCOLOSIS, SMALL POX
AIRBORNE, USE SNUG FIT MASK/ N95
H.INFLUENZAE TYPE B INFECTION, INCLUDING MENINGITIS, PNEUMONIA, EPIGLOTTITIS, SEPSIS
DROPLET USE SURGICAL MASK
COMMON CAUSE OF DIARRHEA, CONTACT PRECAUTION IS ADVICE
C. DIFFICLE
A process whereby all forms of microbial life including bacterial spores are killed
Sterilization (BOICIDE)
Coagulates proteins
Moist heat
Most effective method for sterilization and waste disposal
121° c at 15 lbs pressure per square inch for 15 minutes steam under pressure
Kills viruses and bacteria
Autoclave
Autoclave is used to sterilize the following:
liquid media, instruments, glasswares at:
A 121° c for 15 minutes
B 125° c for 15 minutes
C 121° c for 30 minutes
D125° c for 30 minutes
A. 121° c (250 F) for 15 minutes
Infectious medical waste at_____ for _____minutes allows penetration of the steam throughout the waste and the displacement of air trapped inside the autoclave bag
A. 131° c for 30 to 60 minutes
B. 132° c for 30 to 60 minutes
C. 131° c for 15 to 30 minutes
D. 132° c for 15 to 30 minutes
B 132°c (270 F) for 30 to 60 minutes
Flowing steam 100° c for 30 minutes for three successive days
A. Tyndallization
B. Inspissation
A. Tyndallization
Used for culture media with increased protein
75° c to 80° c for 2 hours for 3 successive days
A. Tyndallization
B. Inspissation
B. Inspissation
Use for sterilizing wire loops and inoculating needles
Flame
Used for glasswares, certain metals, oils, petrolatum, and powders.
160° c to 180° c for 1 and a half to 3 hours
Oven
MOST COMMON method of treating infectious waste,
SAFEST method to ensure that no infective materials remain in samples are containers when disposed
Incineration
Feedback:
PRIONS, infective proteins are NOT ELIMINATED using conventional methods hence INCINERATION is recommended
Used for controlling disease
Cremation
Quality control for autoclave
A. Spores of Bacillus subtilis
B. Spores of Bacillus thearothermophilus
B. Spores of Bacillus thearothermophilus
Quality control for oven
A. Spores of Bacillus subtilis
B. Spores of Bacillus thearothermophilus
A. Spores of Bacillus subtilis
Is a process whereby pathogenic organisms, but not necessarily all microorganisms or spores are destroyed
DISINFECTION (ANTI-SEPSIS)
Disinfectants used on living tissue or skin are called
Antiseptics
Disinfectants at high concentrations used in soaps at low concentrations
Phenolics
Example: phenol, carbolic acid, lysol, hexachlorophene
