Required practical 3

Question 1

What is the purpose of calibration curves.

Answer 1

They are used to determine the concentration of an unkown sample by comparing it to a set of standar values with known concentration.

Question 2

How is a calibration curve used to find the concentration of plant tissue?

Answer 2

Plot a calibration curve of percentage change in mass against concentrationn.

Find the x-intercept where the plant tissue is isotonic to the sucrose solution

Question 3

What occurs when plant tissue is placed in a hypotonic solution?

Answer 3

Water moves into the plant tissue by osmosis, plant tissue increases in mass.

Question 4

What occurs when plant tissue is placed in hypertonic solution?

Answer 4

Water moves out of the plant tissue by osmosis, plant tissue decreases in mass.

Question 5

Why are the potato discs left in solution for 20 mintues?

Answer 5

To allow time for osmosis until the plant tissue reaches equilibrium with its surrounding solution.

Question 6

What is water potential determined by?

Answer 6

The concentration of solutes.

The higher the solute concentration then the lower the water potential.

Question 7

Outline the procedure of investigating osmosis using potato tissue.

Answer 7

1. Make a simple dilution of 1M sucrose to produce 5 concentrations. Add 5cm3 to 5 different test tubes.
2. Cut a potato into equal sized chips and weigh.
3. Place a chip in each test tube and leave for 20 mintues
4. Take out, dab the excess water and weigh them again
5. Calculate the percentage change in mass.

Question 8

Why is percentage change used rather than the actual change in mass?

Answer 8

Potato chips may not all have the same starting mass.

Percentage change allows comparison

Question 9

What is indicated by the x-intercept od the calibration curve?

Answer 9

The concentration that isotonic to the solution tested.

Question 10

Explain the change in mass in the potato chips

Answer 10

The potato chips with concentration lower than the sucrose solution (High water potential) lose mass as there is a net movement of water out of the cells.

The potato chips with concentration higher than the sucrose solution (lower water potential) gain mass as there is a net movement of water into the cells.

Question 11

Why are the potato chips dabbed dry after removing from the sucrose solution?

Answer 11

To remove any excess water clinging to its surface

Question 12

What are the controlled variables of this practical (Investigating osmosis)

Answer 12

• Volume of sucrose solution
• Size of potato chips
• Length of time left in solution
• Dab each potato disc with paper towels.