REAGENT ANTIBODIES

Question 1

Heterogenous population of antibodies produced by different clones of plasma cells

Answer 1

Polyclonal antibodies

Question 2

Homogenous/ uniform population of antibodies produced by single clone of plasma cells

Answer 2

Monoclonal antibodies

Question 3

Difference of polyclonal and monoclonal antibodies in terms of their target

Answer 3

Polyclonal antibodies
- can recognize multiple epitopes of an antigen

Monoclonal antibodies
- can only recognize a single epitope of an antigen

Question 4

Which reagent antibodies require hybridoma cell lines?

Answer 4

Monoclonal antibodies; polyclonal doesn’t require hybridoma cell line

Question 5

Which is more highly specific between polyclonal and monoclonal antibodies and which one is preferred in laboratory testing?

Answer 5

Monoclonal antibodies are HIGHLY SPECIFIC, well characterized and uniformly reactive; PREFERRED IN LABORATORY TESTING

Polyclonal antibodies contains diverse antibodies (NON SPECIFIC) and NOT OPTIMAL IN LABORATORY TESTING

Question 6

Positive sig of an Antigen-Antibody reaction in blood banking:

Answer 6

1. Hemagglutination - most common
2. Hemolysis