BLOOD COMPONENT PREPARATION Flashcards
Centrifugation must be done ____ hours after collection , to allow preparation of RBCs, platelet concentrates. frozen plasma and cryoprecipitate
8-24 hours
Heavy spin for PRBC, and Platelet concentrate:
5,000 x g for 5 minutes
Heavy spin for Cryoprecipitate:
5,000 x g for 7 minutes
Light spin for platelet-rich plasma (PRP)
2,000 x g for 3 minutes
Whole blood
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Whole blood
Indications/uses:
- Symptomatic anemia with large-volume deficit
Storage temp: 1-6C
Shelf-life: depends on Ac
Volume: 450-500 mL
QC for whole blood:
Hct should be at 38-40%
Packed RBC
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Packed RBC
Indications/uses:
- Symptomatic anemia but maybe at risk of circulatory overload
Storage temp: 1-6C
Shelf-life:
- Close System = depend on Ac (same as WB)
- Open System = 24 hours
Volume: 250-300 mL
QC for PRBC:
Hct must be 80% or less
RBC Aliquots
Indications/uses:
Storage temp:
Shelf-life:
Volume:
RBC Aliquots
Indications/uses: used in infants less than 4 months of age
Storage temp: 1-6C
Shelf-life: 24 hours
Volume: 10-25 mL/dose
Most common Ac used in RBC aliquots:
CPDA-1
QC for RBC aliquots:
Hct 80% or less
Leukocyte -Reduced RBC:
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Leukocyte -Reduced RBC:
Indications/uses: prevents febrile reactions; reduces CMV and EBV transmission & HLA alloimmunization; p
Storage temp: 1-6C
Shelf-life:
- Close System = depend on Ac (same as WB)
- Open System = 24 hours
Volume: 250-300 mL
Washed RBCs
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Washed RBCs
Indications/uses: Prevents allergic/anaphylactic reactions; removal of antibodies or other substances
Storage temp: 1-6C
Shelf-life: 24 hours
Volume: 180 mL
QC for Washed RBCs:
Hct: 70-80%
Dosage effect of WB, PRBC, Washed RBCs:
Elevated Hct 3% - 5%
Elevated Hb 1 - 1.5 g/dL
Frozen RBCs
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Frozen RBCs
Indications/uses: For rare phenotyoes and autologous use
Storage temp/shelf life
- Frozen with glycerol: -65C = 10 years
- Deglycerolized units: 1-6C = 24 hours
Volume: 180 mL
Thawing of frozen RBCs:
Thawing is 45 minutes at 37C water bath and washed with decreasing saline concentration
How is platelet concentrate obtained?
Whole blood –> light spin –> PRP –>Heavy spin —> Platelet concentrate
Platelet content of random-donor platelet concentrate VS single-donor platelet concentrate:
Random-donor platelet concentrate: 5.5 x 10^10 platelets
Single-donor platelet concentrate: 3.0 x 10^11 platelets (equivalent to 4-6 random-donor platelets)
Plasma volume of random-donor platelet concentrate VS single-donor platelet concentrate:
Random-donor plt conc: 50-70 mL
Single-donor plt conc: 200-400 mL
Dosage effect of random-donor platelet concentrate VS single-donor platelet concentrate:
Random-donor plt conc:
elevates platelet by 5,000 - 10,000/uL
Single-donor plt conc:
elevates platelet by 30,000 - 60,000/uL
pH of Random and single-donor plt conc.;
pH 6.2 or higher
Shelf-life and storage temperature of random and single-donor plt conc:
5 days / 20-24C with constant agitation
Pooled units of Random-donor plt conc:
4 units
Plasma (FFP, PF 24)
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Plasma (FFP, PF 24)
Indications/uses: Multiple Clotting factor deficiencies, DIC, Vit. K deficiency, Warfarin overdose, Liver disease
Storage temp / Shelf life:
- Frozen at -18C = 1 year
- Frozen at -65C = 7 years
*AFTER THAWING = 1-6C/24 hours
Volume: 200-375 mL
Note:
FFP = plasma frozen within 8 hours of collection
PF 24 = plasma frozen within 24 hours of collection
Cryoprecipitate
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Cryoprecipitate
Indications/uses: Hypofibrinogenemia, Hemophilia A, vWD, F13 deficiency
Storage temp / Shelf life:
- Frozen at -18C = 1 year
*AFTER THAWING = Room temperature/6 hours
Volume: 10-25 mL
Source of Cryoprecipitate:
FFP thawed at 4C then hard spin
Cryoreduced/Cryopoor plasma
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Cryoreduced/Cryopoor plasma
Indications/uses: Plasma from which the cryoprecipitate concentrate has been harvested
Storage temp:
- Frozen at -18C = 1 year shelf life
*AFTER THAWING
= 1-6C/24 hours
= Relabeled plasma may be transfused up to 5 days after it was thawed (Harmening 7th edition)
Volume: 200-380 mL
Granulocyte concentrate
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Granulocyte concentrate
Indications/uses: Corrects severe neutropenia, WBC dysfunctions, myeloid hypoplasia
Storage temp: 20-24C (room temperature)
Shelf-life: 24 hours
Volume: 200 - 600 mL
Irradiated blood
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Irradiated blood
Indications/uses: Immunosuppressed patients that might develop GVHD
Storage temp / shelf life
- RBCs (1-6C) = 28 days
- PLT (20-24C) = 5 days
Volume: varies
Immune serum globulin
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Immune globulin
Indications/uses: Indicated for patients with immunodeficiency diseases and for providing passive antibody prophylaxis
Storage temp: 2-25C
Shelf-life:
- Intramuscular: 3 years
- Intravenous: 1 year
Volume: varies
Normal serum albumin (NSA)
Plasma protein fraction (PPF)
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Normal serum albumin (NSA)
Plasma protein fraction (PPF)
Indications/uses: indicated in patients who are hypovolemic and hypoproteinemic patients
Storage temp: 2-10C
Shelf-life: 5 years
Volume: 50-250 mL
Specialized barcode language recognized by US FDA and mandated by AABB (may 2008); label is 4-by-4inch, made up of four 2-by-3 inch quadrants
ISBT 128 label
Left upper quadrant of ISBT 128 label consist of:
Name of collection facility
Donor identification number (14 characters)
Right upper quadrant of ISBT 128 label:
Blood type (4-digit code)
Lower Left quadrant of ISBT 128 label:
Product Description 98 characters)
Lower Right quadrant of ISBT 128 label:
Special labels
Expiration date
(10 characters)
Color coding of Blood types:
TYPE A:
TYPE B:
TYPE AB:
TYPE O:
Color coding of Blood types:
TYPE A: Blue
TYPE B: Yellow
TYPE AB: Pink
TYPE O: White
