pre transfusion testing Flashcards
Pretransfusion testing begins and ends with the proper _______ of the patient sample
a, identification
b. collection
c. nota
d. aota
answer: identification and collection
Pretransfusion testing begins and ends with the proper _______ of the patient sample
greatest threat to safe transfusion therapy
a. identification
b. collection
c. wrong label
d. clerical error
clerical error
> A major cause of transfusion-associated fatalities is _____ resulting in incorrect ABO groupings and transfusion of ABO incompatible blood.
> Clerical error is the greatest threat to safe transfusion therapy.
> The most common cause of error is misidentification of the recipient
A major cause of transfusion-associated fatalities is _____ resulting in incorrect ABO groupings and transfusion of ABO incompatible blood.
a. identification
b. collection
c. wrong label
d. clerical error
clerical error
> A major cause of transfusion-associated fatalities is _____ resulting in incorrect ABO groupings and transfusion of ABO incompatible blood.
> Clerical error is the greatest threat to safe transfusion therapy.
> The most common cause of error is misidentification of the recipient
In extreme emergencies, when there is no time to obtain and test a pretransfusion sample, group __ can be used
a. o rh + packed cells
b. o rh - packed cells
c. ab rh + packed cells
d. a rh - packed cells
O Rh-negative packed cells
In extreme emergencies, when there is no time to obtain and test a pretransfusion sample, group __ can be used
Both donor and recipient samples must be stored for a minimum of ___ following transfusion
a. 7 days
b. 14 days
c. 4 days
d. 3 days
7 days
Both donor and recipient samples must be stored for a minimum of ___ following transfusion
most critical pre transfusion serologic test
a. identifying the patient
b. correct ABO grouping
c. clerical error
d. misidentification of the recipient
Determining the patient’s correct ABO group is the most critical pretransfusion serologic test
It is a final check of ABO compatibility between donor and patient
crossmatching
final step of pre transfusion compatibility testing
crossmatching
which of these two is for major crossmatching
a. psdr
b. dspr
major crossmatching (PSDR) patient serum+ donor plasma
minor crossmatching (PRDS) patient red cells + donor serum
what are the phases of crossmatching
a. immediate spin
b. antiglobulin phase
c. saline phase
d. incubation phase
phases of crossmatching
- Immediate spin crossmatching / saline phase / initial phase
- incubation phase
- antiglobulin crossmatching phase
which of the 3 phases in crossmatching should be performed in room temperature
a. immediate spin
b. incubation phase
c. antiglobulin phase
immediate spin/ saline phase / initial phase
- done at room temperature
incubation and antiglobulin phase
- done at 37’C
which of the 3 phases in crossmatching use nss as enhancement media
a. immediate spin
b. incubation phase
c. antiglobulin phase
immediate spin
- uses NSS or 22% albumin
incubation phase
- uses LISS
antiglobulin phase
- uses albumin , liss , polyethylene glycol and polybrene
Adding ____________ to the test system reportedly eliminates some of the false-positive reactions
a. liss
b. saline
c. polybrene
d. ethylenediaminetetracetic acid
Adding ethylenediaminetetraacetic acid to the test system reportedly eliminates some of the false-positive reactions
significant antibodies that can be detected in incubation phase
a. IgG
b. IgM
c. IgD
d. IgE
B. Incubation Phase
• Done at 37’C incubation
• Usage of enhancement media such as: LISS -> 15 minutes (5 to 15minutes)
• Detection of clinically significant antibodies such as IgG
An autocontrol, consisting of the_______, may be tested in parallel with the antiglobulin crossmatch test.
a. patient rbc, donor plasma
b. patients own cells and serum
c. donors red cells, patients serum
C.Antiglobulin Crossmatching Phase
- The antiglobulin crossmatch procedure begins in the same manner as the immediate spin crossmatch, continues to a 37°C incubation, and finishes with an antiglobulin test
- Done at 37’C incubation
- Several enhancement media may be applied to boost antigen-antibody reactions. These may include albumin, low ionic strength solution (LISS), polyethylene glycol, and polybrene
- An autocontrol, consisting of the patient’s own cells and serum, may be tested in parallel with the antiglobulin crossmatch test.