ABO Blood Group System Flashcards
WHAT ISTHE SPECIMEN USED IN ABO FORWARD TYPING
A. PATIENT RBC
B. SERUM
C. PLASMA
ABO Forward Typing / Front/ Direct Typing
Specimen: Patient RBC
Reagent: uses commercial antisera, Anti –A (Blue color), Anti- B (Yellow color)
Use: Detection of ABO Antigens
WHAT IS THE USED OF ABO FORWARD TYPING
A. DETECTION OF ABO ANTIBODIES
B. DETECTION OF ABO ANTIGEN
C. NOTA
D. AOTA
ABO Forward Typing / Front/ Direct Typing
Specimen: Patient RBC
Reagent: uses commercial antisera, Anti –A (Blue color), Anti- B (Yellow color)
Use: Detection of ABO Antigens
WHAT IS THE REAGENT USED IN INDIRECT TYPING
A. WHOLE BLOOF
B. ANTI - A , ANTI - B
C. A CELLS , B CELLS
ABO Reverse Typing / Back / Indirect Typing /Serum Typing
Specimen: Serum/plasma
Reagent: A cells, B cells
Use: Detection of ABO antibodies
WHAT IS THE USED OF ABO REVERSE TYPING
A. DETECTION OF ABO ANTIBODIES
B. DETECTION OF ABO ANTIGEN
C. NOTA
D. AOTA
ABO Reverse Typing / Back / Indirect Typing /Serum Typing
Specimen: Serum/plasma
Reagent: A cells, B cells
Use: Detection of ABO antibodies
WHAT IS THE TEMPERATURE OF BLOOD TYPING REAGENT WHEN NOT USED
A. ROOM TEMPERATURE
B. REF TEMPERATURE
C. FROZEN TEMMPERATURE
BLOOD TYPING REAGENTS
WHEN USED ———– ROOM TEMPERATURE
WHEN NOT USED ————- REF TEMPERATURE
WHICH OF THE FOLLOWING PLASMA PRODUCTS IS IDEAL TO BE TRANSFUSED TO A BLOOD TYPE AB PATIENT
a. Plasma product from blood type A donor
b. Plasma product from blood type B donor
c. Plasma product from blood type O donor
d. nota
nota
centrifugation time for blood typing
a. 10 secs
b, 20 secs
c. 30 secs
d. 60 secs
20 secs
universal donor of whole blood or PRBCs
a. blood type O
b. blood type A
c. blood type AB
d. blood type B
B.T O
Universal donor of whole blood or packed RBC
B.T AB
Universal acceptor/recipient of whole blood or packed RBC
B.T AB
Universal donor of plasma products
B.T O
Universal acceptor/recipient of plasma products
Universal acceptor/recipient of plasma products
a. blood type O
b. blood type A
c. blood type AB
d. blood type B
B.T O
Universal donor of whole blood or packed RBC
B.T AB
Universal acceptor/recipient of whole blood or packed RBC
B.T AB
Universal donor of plasma products
B.T O
Universal acceptor/recipient of plasma products
Universal donor of plasma products
a. blood type O
b. blood type A
c. blood type AB
d. blood type B
B.T O
Universal donor of whole blood or packed RBC
B.T AB
Universal acceptor/recipient of whole blood or packed RBC
B.T AB
Universal donor of plasma products
B.T O
Universal acceptor/recipient of plasma products
which of the following is recessive
a. A gene
b. B gene
c. O gene
A and B genes
- DOMINANT
O gene
- Amorph /silent
- Recessive
- No antigen is produced
what is the immunodominant sugar in B gene
a. L-fucose
b. N- acetyl-D- galactosamine
c. D-galactose
d. nota
B gene
GLYCOSYLTRANSFERASE
- D-GALACTOSYLTRANFERASE
IMMUNODOMINAT SUGAR
- D- GALACTOSE
what is the immunodominant sugar in A gene
a. L-fucose
b. N- acetyl-D- galactosamine
c. D-galactose
d. nota
GENE
- A
GLYCOSYLTRANSFERASE
- N - ACETYLGALACTOSYLTRANSFERASE
IMMUNODOMINAT SUGAR
- N-ACETYL-D-GALACTOSEAMINE
amount of H ( from greatest to lowest)
a. O > A1 > A1B > A2 > A2B > B
B. O > A1B > A1 > A2B > B > A2
C. O > A2 > B > A2B > A1 > A1B
D. O > A2B > B > A2 > A1B > A1
AMOUNT OF H ( FROM GREATEST TO LOWEST)
O > A2 > B > A2B > A1 > A1B
what is the origin of type 2 chain
a. plasma
b. erythrocyte precursors
c. serum
type 2
linkage = Beta 1, 4
origin = ERYTHROCYTE PRECURSORS
controlling genes H,A,B
type 1
linkage = Beta 1, 3
origin = plasma
controlling genes = H, A , B ,Se , Lewis
how many forms of H antigen have
a. 1
b. 2
c. 3
d. 4
There are four Forms of H antigen (H1, H2, H3, and H4)
H1 AND H2 = Unbranched straight chain
H3 AND H4 = Complex branched chain
what subgroup a is No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva
a. Am
b. Ay
c. Ael
d. Aend
A3
Mixed field agglutination with anti-A and or anti-AB
Ax
Weak agglutination with anti-AB only
Aend
<10% red cell shows very weak mf agglutination
Am
No agglutination with anti-A and anti-AB, secretors demonstrate QUANTITIES of A substance in saliva
Ay
No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva
Ael
No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva
what subgroup a is <10% red cell shows very weak mf agglutination
a. Am
b. Ay
c. Ael
d. Aend
A3
Mixed field agglutination with anti-A and or anti-AB
Ax
Weak agglutination with anti-AB only
Aend
<10% red cell shows very weak mf agglutination
Am
No agglutination with anti-A and anti-AB, secretors demonstrate QUANTITIES of A substance in saliva
Ay
No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva
Ael
No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva
what subgroup a is No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva
a. Am
b. Ay
c. Ael
d. Aend
A3
Mixed field agglutination with anti-A and or anti-AB
Ax
Weak agglutination with anti-AB only
Aend
<10% red cell shows very weak mf agglutination
Am
No agglutination with anti-A and anti-AB, secretors demonstrate QUANTITIES of A substance in saliva
Ay
No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva
Ael
No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva
what is the source of Anti - B lectin
a. dolichos biflorus
b. bandeiraea simplicifolia
c. ulex europeus
d. aota
Bandeiraea simplicifolia
- source of Anti- B lectin - used to agglutinates B cells that have been pretreated with enzymes
Dolichos biflorus
- source of Anti- A1 lectin
Ulex europeus
- source of anti H lectin
