Polymerase Chain Reaction (PCR)

Question 1

What is Polymerase Chain Reaction (PCR)?

Answer 1

a method of amplifying DNA by artificial replication

in vitro

Question 2

PCR step 1

Answer 2

90* for 30 seconds to denature the DNA by breaking the hydrogen bonds, so strands separate

Question 3

PCR step 2

Answer 3

temp 60* primers anneal to end of DNA strands, to allow strands to replicate

Question 4

PCR step 3

Answer 4

temp 75*
DNA polymerase adds nases to primer building up complementary strands of DNA. so double-stranded DNA identical to the original sequence.
enzyme Taq polymerase

Question 5

Where is PCR enzyme Taq polymerase found?

Answer 5

from thermophilic bacteria in hot springs