Point-of-care Testing, Semi Automated & Automated Blood Cell Analysis

Question 1

The LeukoChek Blood Diluting System
for Manual White Blood Cell and
Platelet Counts Consists of a 20 µL capillary pipette and plastic reservoir containing__________ that makes a 1:100 dilution of whole blood.

Answer 1

1.98 mL of 1% buffered ammonium oxalate

Question 2

In the HemoCue Hb 201 1 System for
Measuring Hemoglobin the hemoglobin is converted to ______ and is read photometrically at two wavelengths _______

Answer 2

570 nm and 880 nm

Question 3

This method avoids the necessity of specimen dilution and interference from turbidity

Answer 3

The HemoCue Hb 201 1 System for
Measuring Hemoglobin

Question 4

Eliminates the use of sealants. They have a factory-inserted plug that seals automatically
when the blood touches the plug.

Answer 4

SUREPREP Capillary Tubes
(Becton, Dickinson)

Question 5

POCT microhematocrit centrifuge- based device

Answer 5

Hematastat II Separation Technology

Question 6

Use the conductivity method to determine the hematocrit. Plasma conducts electrical
current, whereas WBCs act as insulators.

Answer 6

I-STAT Instrument for Measuring Hematocrit

Question 7

Measures total hemoglobin by a spectrophotometric method.

Answer 7

AVOXimeter 1000E

Question 8

Performs a CBC along with platelet aggregation.

Answer 8

Ichor Hematology Analyzer

Question 9

Involves centrifugation in specialized capillary tubes designed to expand the buffy coat layer. The components (platelets, mononuclear cells, and granulocytes) can be measured with the assistance of fluorescent dyes and a measuring device.

Answer 9

Quantitative buffy coat analysis (QBC STAR,
manufactured by QBC Diagnostics, Inc., Philipsburg, PA)

Question 10

TWO BASIC PRINCIPLES OF HEMATOLOGY
ANALYZERS:

Answer 10

● Electronic impedance (resistance)
● Optical scatter

Question 11

Based on the detection and measurement
of changes in electrical resistance produced
by cells as they traverse a small aperture.

Answer 11

EELECTRONIC IMPEDANCE (RESISTANCE)

Question 12

The data are plotted on a frequency
distribution graph, or volume distribution
histogram, with relative number on the y-
axis and volume (channel number equivalent
to a specific volume) on the x-axis.

Answer 12

ELECTRONIC IMPEDANCE (RESISTANCE)

Question 13

Low-voltage DC impedance, as described
previously, may be used in conjunction with
RF resistance, or resistance to a high
voltage electromagnetic current flowing
between both electrodes simultaneously.

Answer 13

Radiofrequency (RF)

Question 14

A hydrodynamically focused sample
stream is directed through a quartz flow
cell past a focused light source.

Answer 14

OPTICAL SCATTER

Question 15

The light source of optical scatter

Answer 15

tungsten-halogen lamp or a helium-neon laser.

Question 16

HEMATOLOGY ANALYZER COMMON BASIC COMPONENTS:

Answer 16

● Hydraulics
● Pneumatics
● Electrical systems

Question 17

includes an aspirating unit, dispensers, diluters, mixing chambers, aperture baths or flow cells or both, and a hemoglobinometer.

Answer 17

Hydraulics

Question 18

generates the vacuums and pressures required for operating the valves and moving the sample through the hydraulics system.

Answer 18

Pneumatics

Question 19

controls operational sequences of the total
system and includes electronic analyzers and computing circuitry for processing the data generated.

Answer 19

Electrical systems