PCR Practical

Question 1

What is the purpose of PCR?

Answer 1

»PCR allows to amplify (several orders of magnitude) a single or a few copies of a gene expressed in a sample of interest

Question 2

What are PCR products separated by?

Answer 2

Charge and size

Question 3

What are the 3 stages of PCR?

Answer 3

Denaturation, Annealing and Elongation

Question 4

What temperature does Denaturation occur at?

Answer 4

94-96 degrees

Question 5

What temperature does Annealing occur at?

Answer 5

68 degrees

Question 6

What temperature does Elongation occur at?

Answer 6

72 degrees

Question 7

Universal ‘forward primers’

Answer 7

Bind to a common sequence at the start of the gene

Question 8

Species-Specific reverse primers

Answer 8

bind to different sequences downstream, each one is designed to produce products of different sizes

Question 9

What do FTA Cards do?

Answer 9

Burst/Lyse Cells, Denature Proteins and purify the nucleic acids

Question 10

Multiplex PCR

Answer 10

Contains multiple primers that all bind to different places in the genome