P1: Steps in Tissue Processing Flashcards

1
Q

Use of an ascending grade of alcohol

A

Dehydration

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2
Q

To preserve the cellular structure and tissue architecture in a life-like manner.

A

Fixation

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3
Q

Removal of water

A

Dehydration

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4
Q

Cross-linking or proteins

A

Fixation

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5
Q

Removal of dehydrating agent

A

Clearing

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6
Q

Leaves tissue in a transparent manner.

A

Wax infiltration

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7
Q

Makes the tissue quite hard and provides a suitable support.

A

Embedding

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8
Q

Xylene

A

Clearing

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9
Q

Osmium tetroxide

A

Fixation

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10
Q

Chloroform

A

Clearing

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11
Q

Paraffin wax

A

Wax infiltration

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12
Q

Paraplast

A

Wax infiltration/Impregnation

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13
Q

Clove oil

A

Clearing

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14
Q

Dioxane

A

Dehydration

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15
Q

Cellosolve

A

Dehydration

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16
Q

Von Ebner’s fluid

A

Decaltification

17
Q

B-5 solution

A

Fixation

18
Q

10% Neutral buffered formalin

A

Fixation

19
Q

Formalin

A

Fixation

20
Q

Ethyl alcohol

A

Dehydration