(P) Lec 1: Bacterial Morphology and Ultrastructure, Physiology, Metabolism, Genetics and Nutrition (Part 1) Flashcards

1
Q

This prefix means that it “cannot be seen with the naked eyes”

A

Micro (penis)

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1
Q

What are the 5 important microbial groups?

A
  1. Prions
  2. Viruses
  3. Bacteria
  4. Fungi
  5. Parasites
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2
Q

In which kingdom do microorganisms belong to?

A

Protista

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3
Q

Microorganisms include what 2 subgroups?

A

Prokaryotes and Eukaryotes

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4
Q

TOF: Not all of the important microbial groups are under the classification of Prokaryote or Eukaryote

A

True (e.g. prions and viruses)

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5
Q

TOF: Prions and viruses are cells

A

False (they are NOT cells)

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6
Q

Identify if Prokaryote or Eukaryote:
Bacteria

A

Prokaryote

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7
Q

Identify if Prokaryote or Eukaryote:
Parasites, Fungi, Plants, and Animals

A

Eukaryote

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8
Q

Identify if Prokaryote or Eukaryote:
Smaller in size

A

Prokaryote

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9
Q

Identify if Prokaryote or Eukaryote:
Larger in size

A

Eukaryote

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10
Q

Identify if Prokaryote or Eukaryote:
Has a true nucleus with a nuclear envelope

A

Eukaryote

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11
Q

Identify if Prokaryote or Eukaryote:
Has no true nucleus

A

Prokaryote

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12
Q

Identify if Prokaryote or Eukaryote:
The cytoplasmic structure is less complex and harbors less structures/organelles

A

Prokaryote

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13
Q

Identify if Prokaryote or Eukaryote:
The cytoplasmic structure is more complex and harbors more structures/organelles

A

Eukaryote

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14
Q

Identify if Prokaryote or Eukaryote:
The cell wall is more complex

A

Prokaryote

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15
Q

Identify if Prokaryote or Eukaryote:
The cell wall is less complex

A

Eukaryote

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16
Q

TOF: Prokaryotes have a nucleoid and nuclear membrane

A

False (no nuclear membrane)

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17
Q

What is the average size range of a prokaryote?

A

Around 1-10 micrometers

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18
Q

These are infectious particles associated with subacute, progressive, and degenerative diseases of the CNS

A

Prions

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19
Q

These are aka abnormally/misfolded proteins

A

Prions

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20
Q

What are examples of diseases caused by prions?

A

Creutzfeldt-Jakob Disease (CJD) and Mad Cow Disease

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21
Q

A degenerative disease of the brain associated with “Kuru” (cannibalism in Papua New Guinea) wherein female tribe members eat the brains of the corpses

A

Creutzfeldt-Jakob Disease (CJD); caused by a prion

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22
Q

What is the smallest among the important microbial groups?

A

Prions

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23
Q

These are acellular, obligate intracellular parasites, that are not visible with the light microscope

A

Viruses

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24
This term means that the parasite cannot live without a host cell, like in the case of viruses
Obligate
25
Since some viruses are enveloped while some are not, when do enveloped viruses start to dissolve their envelopes and multiply?
Once inside the host cell
26
TOF: Viruses contain organelles/biosynthetic machinery along with enzymes
False (no organelles/biosynthetic machinery, only a few enzymes)
27
What do viruses contain as genetic material?
RNA or DNA
28
A virus is called as ______ if it comes with a bacterial host
Bacteriophage
29
TOF: The airborne mode of transmission deals with particles that fall on the ground/surface
False (those are droplets, airborne particles stay in the atmosphere as they are smaller)
30
This is the largest group of organism of medical significance
Bacteria
31
These are unicellular and lack a nuclear membrane and true nucleus
Bacteria
32
TOF: Bacteria are classified as prokaryotic because of the absence of the mitochondria, ER, and golgi bodies (organelles)
True
33
# Bacterial Morphology When asked about bacterial morphology, you are to state the three which are?
1. Shape 2. Arrangement 3. Gram Stain Reaction (+ or -)
34
# Bacterial Morphology (shape) Circular/spherical
Coccus/Cocci
35
# Bacterial Morphology (shape) Short and rod-shaped
Coccobacillus
36
# Bacterial Morphology (shape) Comma-shaped/curved rod
Vibrio
37
# Bacterial Morphology (shape) Rod-shaped
Bacillus/Bacilli
38
# Bacterial Morphology (shape) Curved rod to corkscrew-shaped
Spirillum
39
# Bacterial Morphology (shape) Spiral-shaped and is very small
Spirochetes
40
# Bacterial Morphology (shape) This can only be visualized via darkfield microscopy (e.g. Treponema spp.; causative agent of syphilis)
Spirochete
41
# Bacterial Morphology (arrangement) What do you call Cocci that are in pairs?
Diplococci
42
# Bacterial Morphology (arrangement) What is an example of Cocci in chains/bead-formation?
Streptococci
43
# Bacterial Morphology (arrangement) What do you call Cocci that are in groups of 4?
Tetrads
44
# Bacterial Morphology (arrangement) What do you call Cocci that are in groups of 8?
Sarcina
45
# Bacterial Morphology (arrangement) What is an example of Cocci in clusters/grape-formation?
Staphylococci
46
# Bacterial Morphology (arrangement) What do you call lone Coccus?
Singly
47
# Bacterial Morphology (arrangement) What do you call lone Bacillus?
Singly
48
# Bacterial Morphology (arrangement) What is an example of Bacilli in palisading/picket fence formation?
Corynebacterium diphtheriae
49
# Gram Staining Refers to air drying the sample and using heat to let it adhere to the slide
Heat-fixed cells
50
# Gram Staining The primary stain
Crystal Violet
51
# Gram Staining This accentuates the primary stain to attach better to the bacteria (a mordant)
Gram's Iodine
52
# Gram Staining Aka the decolorizer
Ethyl Alcohol
53
# Gram Staining Aka the counterstain
Safranin
54
# Gram Staining (identify if positive or negative) After administering crystal violet: Purple
Both (+) and (-)
55
# Gram Staining (identify if positive or negative) After administering Gram's iodine: Purple
Both (+) and (-)
56
# Gram Staining (identify if positive or negative) After administering alcohol: Purple
Positive
57
# Gram Staining (identify if positive or negative) After administering alcohol: Colorless
Negative
58
# Gram Staining (identify if positive or negative) After administering safranin: Purple
Positive
59
# Gram Staining (identify if positive or negative) After administering safranin: Pink
Negative
60
Aka "differential staining" wherein the bacteria are identified to be positive or negative
Gram Staining
61
# Gram Staining How long is crystal violet stained for?
30 seconds
62
# Gram Staining How long is iodine administered for?
1 minute
63
# Gram Staining How long is alcohol administered for?
5-15 seconds
64
# Gram Staining How long is safranin stained for?
1 minute
65
# Gram Staining (identify if positive or negative) This organism's cell wall is 50% of the dry weight of the cell envelope
Positive
66
# Gram Staining (identify if positive or negative) This organism's cell wall is 2-10% of the dry weight of the cell envelope
Negative
67
All cocci are what gram stain? (except for some)
Positive (circles are purple)
68
What cocci bacteria are not gram-positive? (the exemptions)
1. Neisseria 2. Veilonella 3. Moraxella (Mnemonics: NVM)
69
All bacilli are what gram stain? (except for some)
Negative (rods are red/pink)
70
What bacilli bacteria are not gram-negative? (the exemptions)
1. Mycobacterium 2. Corynebacterium 3. Bacillus 4. Clostridium 5. Actinomyces 6. Streptomyces Less common ones: 1. Erysipelothrix 2. Listeria 3. Nocardia 4. Anything ending in bacterium (e.g. Proprionibacterium, Eubacterium, and Bifidobacterium)
71
TOF: The bacterial structure is important for its pathogenesis and virulence factors
True
72
# Cell Envelope What are the 4 parts of the cell envelope?
1. Outer membrane 2. Cell wall 3. Periplasmic space 4. Cytoplasmic (cell) membrane
73
# Cell Envelope The outer membrane only exists for what type of bacteria?
Gram-Negative
74
# Cell Envelope This is the initial barrier to the environment, it is a bilayer composed of lipopolysaccharides
Outer Membrane
75
# Cell Envelope The outer membrane consists of this substance which functions as an endotoxin
Lipid A (note: endotoxins are for gram-negative while exotoxins are for gram-positive)
76
# Cell Envelope The outer membrane consists of this substance which contains antigens (H and O antigens) and can be used for serologic typing
Major surface antigenic determinants
77
# Cell Envelope The outer membrane consists of this water-filled structure that controls the passage of nutrients and other solutes
Porins
78
# Cell Envelope Aka "Murein" or the "Peptidoglycan Layer"
Cell Wall
79
# Cell Envelope This gives the cell its shape and strength; it is the site of action for antibiotics, and it is also the basis of staining
Cell Wall
80
# Cell Envelope The cell wall is composed of what subunits?
Disaccharide-Pentapeptide Subunits (N-acetylglucosamide and N-acetylmuramic acid)
81
# Cell Envelope TOF: Some bacteria have jelly cell walls made up of mycolic acid
False (waxy, not jelly)
82
# Cell Envelope Bacteria that have waxy cell walls have this key component that contributes to their virulence hence becoming harder to kill/penetrate
Mycolic Acid
83
# Cell Envelope What is an example of a bacterium with a waxy cell wall?
Mycobacterium spp. (takes 6 months to treat)
84
# Cell Envelope This consists of gel-like substances that assist in the capture of nutrients from the environment; it contains enzymes that degrade macromolecules and detoxifies evironmental solutes
Periplasmic Membrane
85
# Cell Envelope Not all bacteria are encapsulated, hence why they are called as?
An appendage
86
# Cell Envelope The periplasmic membrane space contains these which are responsible for metabolism and detoxification
Enzymes
87
# Cell Envelope This contains enzymes vital to cellular metabolism and serves as an additional osmotic barrier
Cell Membrane
88
# Cell Envelope This transports solute into and out of the cell, it houses enzymes involved in the synthesis of the outer membrane and cell wall, it aids in the assembly and secretion of extracytoplasmic and extracellular substances, and it generates chemical energy
Cell Membrane
89
# Cell Appendages This is composed of high molecular weight polysaccharides, it is anti-phagocytic, and it facilitates and maintains bacterial colonization of biologic and inanimate surfaces
Capsule
90
# Cell Appendages The capsule facilitates and maintains bacterial colonization of biologic and inanimate surfaces through the formation of what?
Slime layers or biofilms
91
# Cell Appendages TOF: When an organism is encapsulated, it is non-motile
True
92
# Cell Appendages These promote the uptake of the pathogen by a macrophage by inducing phagocytosis (microbial recognition and killing of encapsulated bacteria)
Opsonins
93
# Cell Appendages The Bacillus anthracis capsule is made up of what?
Poly-D-glutamic acid (PGA)
94
# Cell Appendages This is the causative agent of anthrax
Bacillus anthracis
95
# Cell Appendages What is the more common form of anthrax found in cows and sheep?
Cutaneous anthrax
96
# Cell Appendages The capsule's slime layer is aka?
Glycocalyx
97
# Cell Appendages This capsule component is loosely adherent to the bacteria (functions as an adhesive) composed of polysaccharide fibrils surrounding some bacterial cell walls
Slime Layer/ Glycocalyx
98
# Cell Appendages These capsule components are aggregates of bacterial cells which enhance nutrient uptake and often excludes antimicrobials
Biofilm
99
# Cell Appendages Organisms can produce these on medical implant devices (e.g. heart valves, prostheses, and catheters) which can cause diseases
Biofilms
100
# Cell Appendages This common biofilm producer is a normal inhabitant of the skin but is seen in catheter-related infections
Staphylococcus epidermidis
101
# Cell Appendages This common biofilm producer is a normal inhabitant of the mouth but is seen in cases of dental plaques
Streptococcus mutans
102
What are the representative encapsulated organisms? (YSKBHPNC)
1. Yersinia pestis 2. Streptococcus pneumoniae 3. Klebsiella pneumoniae 4. Bacillus anthracis 5. Haemophilus influenza 6. Pseudomonas aeruginosa 7. Neisseria meningitidis 8. Cryptococcus neoformans
103
This representative encapsulated organism was the causative agent for the black plague
Yersinia pestis
104
What are the 4 other important capsule producers?
1. Escherichia coli 2. Bacillus anthracis 3. Salmonella enteritica subsp. typhi 4. Streptococcus pneumoniae
105
# Cell Appendages These are hair-like proteinaceous structures that extend from the cell membrane into the external environment and may serve as adhesins
Fimbriae or Pili
106
# Cell Appendages What are the 2 types of fimbriae/pili?
1. Common pili 2. Sex pili
107
# Cell Appendages This type of pili are involved in bacterial adherence and gram-positive cell conjugation
Common pili (common = positive)
108
# Cell Appendages This type of pili are involved in the attachment of donor and recipient bacteria in gram-negative cell conjugation
Sex pili (sex = negative)
109
# Cell Appendages These participate in the conjugation of bacteria (mechanism of gene transfer)
Fimbriae/Pili
110
# Cell Appendages This requires cell-to-cell contact, and through which genes may be transferred from one bacterial cell to another (ex: genes for antimicrobial resistance)
Conjugation
111
# Cell Appendages TOF: Fimbriae/Pili are for motility
False
112
# Cell Appendages Functions include motility, chemotaxis, and bacterial pathogenicity
Flagella
113
# Cell Appendages The flagella is composed of what protein?
Flagellin
114
# Cell Appendages Kind of bacteria based on flagella presentation: A single flagellum at one end of the cell
Monotrichous
115
# Cell Appendages Kind of bacteria based on flagella presentation: A group of flagella at one end of the cell
Lophotrichous
116
# Cell Appendages Kind of bacteria based on flagella presentation: Single flagellum at both ends of the cell
Amphitrichous
117
# Cell Appendages Kind of bacteria based on flagella presentation: The entire cell surface covered with flagella
Peritrichous
118
# Cell Interior Contains (+) enzymes for metabolism and detoxification and is the site for protein synthesis
Cytoplasm
119
# Cell Interior TOF: The cytoplasm of bacteria contains golgi bodies
False
120
# Cell Interior If a cell component has a gel-like structure (e.g. cytoplasm) it has what?
Enzymes
121
# Cell Interior Serves as the nutrient reservoir for the bacteria
Inclusion bodies
122
# Cell Interior The cell's inclusion bodies contain?
Glycogen and Polyphosphate Granules
123
# Cell Interior What is the storage form of glucose and inorganic phosphates? (2 answers)
Glycogen and Polyphosphate Granules
124
# Cell Interior Inclusion bodies are visualized using what methods?
Staining methods
125
# Cell Interior Corynebacteria's characteristic feature is the presence of what?
Metachromatic or Volutin Granules (appears in a pallisading arrangement and beaded)
126
# Cell Interior Invaginations in the bacterial cell structure
Mesosomes
127
# Cell Interior Highly coiled DNA intermixed with RNA, polyamines, and various proteins
Nucleus/Nucleoid
128
# Cell Interior TOF: Bacterial chromosomes are enclosed within a membrane-bound nucleus
False (it exists as a nucleoid)
129
# Cell Interior Small, circular, extrachromasomal (nonchromosomal) dsDNA capable of self-replication which becomes integrated into the bacterial DNA
Plasmids
130
# Cell Interior Plasmids contains these genes that can confer protective properties (3 answers)
1. Antibiotic resistance 2. Virulence factors 3. Transmissibility to other bacteria
131
# Cell Interior A process by which cells transform from an actively metabolic and growing state to a dormant state
Sporulation
132
# Cell Interior This happens to some bacteria when they are exposed to unfavorable conditions (excessive dryness, nutrient scarcity, toxin accumulation, etc.)
Sporulation
133
# Cell Interior This process induces an increase in cell envelope thickness & strength (a keratin coat)
Sporulation
134
# Cell Interior Are dormant, thick-walled structures produced by some bacteria that allow them to survive for long periods of time
Endospores
135
# Cell Interior What are endospores made out of?
Calcium Dipicolinate
136
# Cell Interior What are the only 2 bacterial genera that can produce spores?
Bacillus and Clostridium
137
# Cell Interior TOF: Spores do not have resistance to heat, dessication (extreme dryness), and various chemicals
False
138
# Cell Interior Endospores will remain in a dormant state until favorable conditions for what are encountered again?
Growth
139
# Cell Interior This bacteria emerges from its dormant state once ingested and into the GIT which will eventually cause food poisoning
Bacillus cereus