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Clinical Bacteriology > (F) Lab: Biochemical ID of Enterobacteriaceae > Flashcards

(F) Lab: Biochemical ID of Enterobacteriaceae Flashcards

1
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/K
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Escherichia coli

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2
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose, Lactose, Maltose: (+)
A

Klebsiella pneumoniae

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3
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Enterobacter aerogenes

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4
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Enterobacter cloacae

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5
Q

Identify microorganism

  • TSI: A/A + Gas + H2S
  • LIA: K/K + H2S
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Arizona spp.

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6
Q

Identify microorganism

  • TSI: A/A + Gas + H2S
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Citrobacter freundii

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7
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Citrobacter diversus

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8
Q

Identify microorganism

  • TSI: K/A + Gas + H2S
  • LIA: R/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Proteus vulgaris

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9
Q

Identify microorganism

  • TSI: K/A + Gas + H2S
  • LIA: R/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Proteus mirabilis

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10
Q

Identify microorganism

  • TSI: K/A
  • LIA: R/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (+)
A

Providencia rettgeri

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11
Q

Identify microorganism

  • TSI: K/A
  • LIA: R/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Morganella morganii

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12
Q

Identify microorganism

  • TSI: K/A + H2S (small)
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Salmonella typhi

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13
Q

Identify microorganism

  • TSI: K/A + H2S (large)
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Salmonella typhimurium

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14
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Shigella dysenteriae

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15
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (+)
A

Shigella flexneri/boydii

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16
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Shigella sonnei

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17
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/K
  • Indole: (+)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (-)
  • Urease: (+)
  • Dextrose, Lactose, Maltose: (+)
A

Serratia marcescens

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18
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Green/Green
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Pseudomonas aeruginosa

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19
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Blue/Blue
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Alkaligenes faecalis

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20
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Blue/Green + Yellow band
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Acinetobacter baumanii

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21
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Blue/Green
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Acinetobacter lwoffi

22
Q

Give the 4 rapid lactose fermenters

A
  1. Escherichia coli
  2. Klebsiella pneumoniae
  3. Enterobacter aerogenes
  4. Enterobacter cloacae
23
Q

Give the 3 late lactose fermenters

A
  1. Arizona spp.
  2. Citrobacter freundii
  3. Citrobacter diversus
24
Q

Give the 4 non-lactose fermenters of the Proteae tribe

A
  1. Proteus vulgaris
  2. Proteus mirabilis
  3. Providencia rettgeri
  4. Morganella morganii
25
Q

Give the 2 non-lactose fermenters of the Salmonella spp.

A
  1. Salmonella typhi
  2. Salmonella typhimurium
26
Q

Give the 4 non-lactose fermenters of the Shigella spp.

A
  1. Shigella dysenteriae
  2. Shigella flexneri
  3. Shigella boydii
  4. Shigella sonnei
27
Q

Give the 4 non-fermenters that do not belong under Enterobacteriaceae

A
  1. Pseudomonas aeruginosa
  2. Alkaligenes faecalis
  3. Acinetobacter baumanii
  4. Acinetobacter lwoffi
28
Q

Give all of the lactose fermenters (whether rapid or late)

A
  1. Escherichia coli
  2. Klebsiella pneumoniae
  3. Enterobacter aerogenes
  4. Enterobacter cloacae
  5. Arizona spp.
  6. Citrobacter freundii
  7. Citrobacter diversus
29
Q

Give all of the non-lactose fermenters (regardless of tribe)

A
  1. Proteus vulgaris
  2. Proteus mirabilis
  3. Providencia rettgeri
  4. Morganella morganii
  5. Salmonella typhi
  6. Salmonella typhimurium
  7. Shigella dysenteriae
  8. Shigella flexneri
  9. Shigella boydii
  10. Shigella sonnei
  11. Pseudomonas aeruginosa
  12. Alkaligenes faecalis
  13. Acinetobacter baumanii
  14. Acinetobacter lwoffi
30
Q

What organism is the only indole (+) in the rapid lactose fermenters group?

A

Escherichia coli

31
Q

What organism is the only LIA: K/A in the rapid lactose fermenters group?

A

Enterobacter cloacae

32
Q

What organism is the only MR (+) and VP (-) in the rapid lactose fermenters group?

A

Escherichia coli

33
Q

What organism is the only Urease (+) in the rapid lactose fermenters group?

A

Klebsiella pneumonia

34
Q

What organism is the only Citrate (-) in the lactose fermenters group whether rapid or late?

A

Escherichia coli

35
Q

This pattern of TSI and LIA alone is exclusive for what organism?
- TSI: A/A + Gas + H2S
- LIA: K/K + H2S

A

Arizona spp.

36
Q

This pattern of TSI and LIA alone is exclusive for what organism?
- TSI: A/A + Gas + H2S
- LIA: K/A

A

Citrobacter freundii

37
Q

What is the only late lactose fermenter that DOESN’T produce H2S in TSI?

A

Citrobacter diversus

38
Q

What is the only Indole (+) organism in the late lactose fermenters group?

A

Citrobacter diversus

39
Q

What are the 2 members of the Proteae tribe that produce Gas and H2S in their TSI?

A
  1. Proteus vulgaris
  2. Proteus mirabilis
40
Q

What is the only Indole (-) member of the Proteae tribe?

A

Proteus mirabilis

41
Q

What is the only Citrate (-) member of the Proteae tribe?

A

Morganella morganii

42
Q

What is the only Maltose (+) member of the Proteae tribe?

A

Providencia rettgeri

43
Q

What is the test that differentiates S. typhi from S. typhimurium?

A

Citrate

44
Q

What is the Salmonella spp. member that is Citrate (-)?

A

Salmonella typhi

45
Q

What is the Salmonella spp. member that is Citrate (+)?

A

Salmonella typhimurium

46
Q

What are the 2 Shigella spp. members that are Indole (+)?

A

Shigella flexneri and boydii

47
Q

All Proteae tribe members test positive for what sugar?

A

Dextrose

48
Q

What is the only Lactose (+) non-lactose fermenter?

A

Shigella sonnei

49
Q

What is the only Maltose (-) in the Shigella spp. group?

A

Shigella dystenteriae

50
Q

Enterobacter aerogenes and cloacae are identical in their battery of tests except for 1, which test it is?

A

Lysine Iron Agar
- E. aerogenes: K/K
- E. cloacae: K/A

Clinical Bacteriology (33 decks)

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