(F) Lab: Biochemical ID of Enterobacteriaceae Flashcards
Identify microorganism
- TSI: A/A + Gas
- LIA: K/K
- Indole: (+)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (-)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Escherichia coli
Identify microorganism
- TSI: A/A + Gas
- LIA: K/K
- Indole: (-)
- Methyl Red: (-)
- Vogues-Proskauer: (+)
- Citrate: (+)
- Urease: (+)
- Dextrose, Lactose, Maltose: (+)
Klebsiella pneumoniae
Identify microorganism
- TSI: A/A + Gas
- LIA: K/K
- Indole: (-)
- Methyl Red: (-)
- Vogues-Proskauer: (+)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Enterobacter aerogenes
Identify microorganism
- TSI: A/A + Gas
- LIA: K/A
- Indole: (-)
- Methyl Red: (-)
- Vogues-Proskauer: (+)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Enterobacter cloacae
Identify microorganism
- TSI: A/A + Gas + H2S
- LIA: K/K + H2S
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Arizona spp.
Identify microorganism
- TSI: A/A + Gas + H2S
- LIA: K/A
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Citrobacter freundii
Identify microorganism
- TSI: A/A + Gas
- LIA: K/A
- Indole: (+)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Citrobacter diversus
Identify microorganism
- TSI: K/A + Gas + H2S
- LIA: R/A
- Indole: (+)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (+)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (-)
Proteus vulgaris
Identify microorganism
- TSI: K/A + Gas + H2S
- LIA: R/A
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (+)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (-)
Proteus mirabilis
Identify microorganism
- TSI: K/A
- LIA: R/A
- Indole: (+)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (+)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (+)
Providencia rettgeri
Identify microorganism
- TSI: K/A
- LIA: R/A
- Indole: (+)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (-)
- Urease: (+)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (-)
Morganella morganii
Identify microorganism
- TSI: K/A + H2S (small)
- LIA: K/K
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (-)
- Urease: (-)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (-)
Salmonella typhi
Identify microorganism
- TSI: K/A + H2S (large)
- LIA: K/K
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (-)
Salmonella typhimurium
Identify microorganism
- TSI: K/A
- LIA: K/A
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (-)
- Urease: (-)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (-)
Shigella dysenteriae
Identify microorganism
- TSI: K/A
- LIA: K/A
- Indole: (+)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (-)
- Urease: (-)
- Dextrose: (+)
- Lactose: (-)
- Maltose: (+)
Shigella flexneri/boydii
Identify microorganism
- TSI: K/A
- LIA: K/A
- Indole: (-)
- Methyl Red: (+)
- Vogues-Proskauer: (-)
- Citrate: (-)
- Urease: (-)
- Dextrose, Lactose, Maltose: (+)
Shigella sonnei
Identify microorganism
- TSI: K/A
- LIA: K/K
- Indole: (+)
- Methyl Red: (-)
- Vogues-Proskauer: (+)
- Citrate: (-)
- Urease: (+)
- Dextrose, Lactose, Maltose: (+)
Serratia marcescens
Identify microorganism
- TSI: K/K
- Seller’s: Green/Green
- Indole: (-)
- Methyl Red: (-)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (-)
Pseudomonas aeruginosa
Identify microorganism
- TSI: K/K
- Seller’s: Blue/Blue
- Indole: (-)
- Methyl Red: (-)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (-)
Alkaligenes faecalis
Identify microorganism
- TSI: K/K
- Seller’s: Blue/Green + Yellow band
- Indole: (-)
- Methyl Red: (-)
- Vogues-Proskauer: (-)
- Citrate: (+)
- Urease: (-)
- Dextrose, Lactose, Maltose: (-)
Acinetobacter baumanii