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Clinical Bacteriology > (F) Lab: Biochemical ID of Enterobacteriaceae > Flashcards

(F) Lab: Biochemical ID of Enterobacteriaceae Flashcards

1
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/K
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Escherichia coli

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2
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose, Lactose, Maltose: (+)
A

Klebsiella pneumoniae

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3
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Enterobacter aerogenes

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4
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Enterobacter cloacae

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5
Q

Identify microorganism

  • TSI: A/A + Gas + H2S
  • LIA: K/K + H2S
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Arizona spp.

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6
Q

Identify microorganism

  • TSI: A/A + Gas + H2S
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Citrobacter freundii

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7
Q

Identify microorganism

  • TSI: A/A + Gas
  • LIA: K/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Citrobacter diversus

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8
Q

Identify microorganism

  • TSI: K/A + Gas + H2S
  • LIA: R/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Proteus vulgaris

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9
Q

Identify microorganism

  • TSI: K/A + Gas + H2S
  • LIA: R/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Proteus mirabilis

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10
Q

Identify microorganism

  • TSI: K/A
  • LIA: R/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (+)
A

Providencia rettgeri

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11
Q

Identify microorganism

  • TSI: K/A
  • LIA: R/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (+)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Morganella morganii

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12
Q

Identify microorganism

  • TSI: K/A + H2S (small)
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Salmonella typhi

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13
Q

Identify microorganism

  • TSI: K/A + H2S (large)
  • LIA: K/K
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Salmonella typhimurium

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14
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (-)
A

Shigella dysenteriae

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15
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/A
  • Indole: (+)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose: (+)
  • Lactose: (-)
  • Maltose: (+)
A

Shigella flexneri/boydii

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16
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/A
  • Indole: (-)
  • Methyl Red: (+)
  • Vogues-Proskauer: (-)
  • Citrate: (-)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (+)
A

Shigella sonnei

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17
Q

Identify microorganism

  • TSI: K/A
  • LIA: K/K
  • Indole: (+)
  • Methyl Red: (-)
  • Vogues-Proskauer: (+)
  • Citrate: (-)
  • Urease: (+)
  • Dextrose, Lactose, Maltose: (+)
A

Serratia marcescens

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18
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Green/Green
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Pseudomonas aeruginosa

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19
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Blue/Blue
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Alkaligenes faecalis

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20
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Blue/Green + Yellow band
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Acinetobacter baumanii

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21
Q

Identify microorganism

  • TSI: K/K
  • Seller’s: Blue/Green
  • Indole: (-)
  • Methyl Red: (-)
  • Vogues-Proskauer: (-)
  • Citrate: (+)
  • Urease: (-)
  • Dextrose, Lactose, Maltose: (-)
A

Acinetobacter lwoffi

22
Q

Give the 4 rapid lactose fermenters

A
  1. Escherichia coli
  2. Klebsiella pneumoniae
  3. Enterobacter aerogenes
  4. Enterobacter cloacae
23
Q

Give the 3 late lactose fermenters

A
  1. Arizona spp.
  2. Citrobacter freundii
  3. Citrobacter diversus
24
Q

Give the 4 non-lactose fermenters of the Proteae tribe

A
  1. Proteus vulgaris
  2. Proteus mirabilis
  3. Providencia rettgeri
  4. Morganella morganii
25
Give the 2 non-lactose fermenters of the Salmonella spp.
1. Salmonella typhi 2. Salmonella typhimurium
26
Give the 4 non-lactose fermenters of the Shigella spp.
1. Shigella dysenteriae 2. Shigella flexneri 3. Shigella boydii 4. Shigella sonnei
27
Give the 4 non-fermenters that do not belong under Enterobacteriaceae
1. Pseudomonas aeruginosa 2. Alkaligenes faecalis 3. Acinetobacter baumanii 4. Acinetobacter lwoffi
28
Give all of the lactose fermenters (whether rapid or late)
1. Escherichia coli 2. Klebsiella pneumoniae 3. Enterobacter aerogenes 4. Enterobacter cloacae 5. Arizona spp. 6. Citrobacter freundii 7. Citrobacter diversus
29
Give all of the non-lactose fermenters (regardless of tribe)
1. Proteus vulgaris 2. Proteus mirabilis 3. Providencia rettgeri 4. Morganella morganii 5. Salmonella typhi 6. Salmonella typhimurium 7. Shigella dysenteriae 8. Shigella flexneri 9. Shigella boydii 10. Shigella sonnei 11. Pseudomonas aeruginosa 12. Alkaligenes faecalis 13. Acinetobacter baumanii 14. Acinetobacter lwoffi
30
What organism is the only indole (+) in the rapid lactose fermenters group?
Escherichia coli
31
What organism is the only LIA: K/A in the rapid lactose fermenters group?
Enterobacter cloacae
32
What organism is the only MR (+) and VP (-) in the rapid lactose fermenters group?
Escherichia coli
33
What organism is the only Urease (+) in the rapid lactose fermenters group?
Klebsiella pneumonia
34
What organism is the only Citrate (-) in the lactose fermenters group whether rapid or late?
Escherichia coli
35
This pattern of TSI and LIA alone is exclusive for what organism? - TSI: A/A + Gas + H2S - LIA: K/K + H2S
Arizona spp.
36
This pattern of TSI and LIA alone is exclusive for what organism? - TSI: A/A + Gas + H2S - LIA: K/A
Citrobacter freundii
37
What is the only late lactose fermenter that DOESN'T produce H2S in TSI?
Citrobacter diversus
38
What is the only Indole (+) organism in the late lactose fermenters group?
Citrobacter diversus
39
What are the 2 members of the Proteae tribe that produce Gas and H2S in their TSI?
1. Proteus vulgaris 2. Proteus mirabilis
40
What is the only Indole (-) member of the Proteae tribe?
Proteus mirabilis
41
What is the only Citrate (-) member of the Proteae tribe?
Morganella morganii
42
What is the only Maltose (+) member of the Proteae tribe?
Providencia rettgeri
43
What is the test that differentiates S. typhi from S. typhimurium?
Citrate
44
What is the Salmonella spp. member that is Citrate (-)?
Salmonella typhi
45
What is the Salmonella spp. member that is Citrate (+)?
Salmonella typhimurium
46
What are the 2 Shigella spp. members that are Indole (+)?
Shigella flexneri and boydii
47
All Proteae tribe members test positive for what sugar?
Dextrose
48
What is the only Lactose (+) non-lactose fermenter?
Shigella sonnei
49
What is the only Maltose (-) in the Shigella spp. group?
Shigella dystenteriae
50
Enterobacter aerogenes and cloacae are identical in their battery of tests except for 1, which test it is?
Lysine Iron Agar - E. aerogenes: K/K - E. cloacae: K/A

Clinical Bacteriology (33 decks)

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